Hubei dimarhabdovirus virus 1

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KMV4|A0A1L3KMV4_9VIRU Nucleocapsid protein OS=Hubei dimarhabdovirus virus 1 OX=1922866 PE=4 SV=1
MM1 pKa = 7.9SFEE4 pKa = 4.41TEE6 pKa = 4.3SAHH9 pKa = 7.15HH10 pKa = 6.57VDD12 pKa = 3.97LAEE15 pKa = 4.38SLVKK19 pKa = 9.98TLTSMQKK26 pKa = 9.55NQTLSKK32 pKa = 10.86LPVFDD37 pKa = 5.82DD38 pKa = 3.86SSDD41 pKa = 3.57MTDD44 pKa = 3.42EE45 pKa = 4.4DD46 pKa = 4.86HH47 pKa = 7.77SNSDD51 pKa = 3.61PDD53 pKa = 3.88VVGAQYY59 pKa = 11.06LGDD62 pKa = 3.83PAEE65 pKa = 4.89LFGSLPPAVARR76 pKa = 11.84ATPQSLDD83 pKa = 3.47LSFLSLSDD91 pKa = 3.37AKK93 pKa = 10.4RR94 pKa = 11.84VQDD97 pKa = 5.25LICSLDD103 pKa = 3.28TSRR106 pKa = 11.84PIDD109 pKa = 4.16EE110 pKa = 4.97IEE112 pKa = 4.13FVLPSAPRR120 pKa = 11.84KK121 pKa = 9.56PSTPKK126 pKa = 9.83PSAPVVTEE134 pKa = 3.48QRR136 pKa = 11.84QPPKK140 pKa = 10.61ASSTSKK146 pKa = 9.44TRR148 pKa = 11.84MVKK151 pKa = 10.64LGILLEE157 pKa = 4.42KK158 pKa = 10.36GVEE161 pKa = 4.09VRR163 pKa = 11.84SRR165 pKa = 11.84YY166 pKa = 8.74PDD168 pKa = 3.44KK169 pKa = 11.15PPFKK173 pKa = 10.12IYY175 pKa = 10.55NGRR178 pKa = 11.84NGFSKK183 pKa = 10.35EE184 pKa = 3.69QLAARR189 pKa = 11.84YY190 pKa = 7.58QNRR193 pKa = 11.84EE194 pKa = 4.08LPEE197 pKa = 3.73TLYY200 pKa = 11.22DD201 pKa = 3.61VVKK204 pKa = 10.83EE205 pKa = 4.11IFTEE209 pKa = 4.22AKK211 pKa = 9.13NWGVFINRR219 pKa = 11.84YY220 pKa = 8.17HH221 pKa = 7.28AALLKK226 pKa = 10.59INLDD230 pKa = 3.67QYY232 pKa = 11.22SCEE235 pKa = 3.99

Molecular weight: 26.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KMY8|A0A1L3KMY8_9VIRU GDP polyribonucleotidyltransferase OS=Hubei dimarhabdovirus virus 1 OX=1922866 PE=4 SV=1
MM1 pKa = 7.51KK2 pKa = 10.13PITTALKK9 pKa = 10.2KK10 pKa = 10.32IRR12 pKa = 11.84GSPGAIRR19 pKa = 11.84SRR21 pKa = 11.84AQTTLPSLPPKK32 pKa = 10.37DD33 pKa = 3.98LRR35 pKa = 11.84CSKK38 pKa = 9.9WSIVAEE44 pKa = 4.16MKK46 pKa = 9.67LTLSQEE52 pKa = 4.01ALDD55 pKa = 3.73WEE57 pKa = 4.64IVQRR61 pKa = 11.84IAGVHH66 pKa = 5.5RR67 pKa = 11.84DD68 pKa = 3.67EE69 pKa = 4.63YY70 pKa = 10.71TGNPYY75 pKa = 9.79FRR77 pKa = 11.84PIIEE81 pKa = 4.04TFYY84 pKa = 10.54WLMLIHH90 pKa = 6.41TKK92 pKa = 10.4KK93 pKa = 10.94DD94 pKa = 3.1NTQITNSTTYY104 pKa = 10.71SSVFTEE110 pKa = 4.55LVSVHH115 pKa = 6.43HH116 pKa = 6.59NIPLVSNQDD125 pKa = 3.13FSYY128 pKa = 10.49SYY130 pKa = 9.12CTSGIHH136 pKa = 5.98RR137 pKa = 11.84HH138 pKa = 4.46TTFTIQFRR146 pKa = 11.84VKK148 pKa = 9.06FTPTVRR154 pKa = 11.84RR155 pKa = 11.84TEE157 pKa = 3.83DD158 pKa = 2.91LGFFLEE164 pKa = 4.6RR165 pKa = 11.84KK166 pKa = 8.32PFYY169 pKa = 10.16WSDD172 pKa = 3.77GPDD175 pKa = 3.36VIQVLKK181 pKa = 10.72MEE183 pKa = 4.75GLHH186 pKa = 6.87IALCGDD192 pKa = 4.22LLVIIDD198 pKa = 4.07PEE200 pKa = 4.32KK201 pKa = 10.9NN202 pKa = 3.1

Molecular weight: 23.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

3543

202

2135

708.6

80.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.403 ± 0.508

1.665 ± 0.16

5.588 ± 0.404

5.25 ± 0.175

4.601 ± 0.154

5.532 ± 0.55

2.653 ± 0.157

7.141 ± 0.645

6.125 ± 0.126

10.443 ± 0.5

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.145 ± 0.258

5.25 ± 0.396

4.968 ± 0.696

3.189 ± 0.176

5.109 ± 0.472

7.988 ± 0.71

7.141 ± 0.474

5.306 ± 0.409

1.524 ± 0.143

3.98 ± 0.137
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski