Calycomorphotria hydatis

Taxonomy: cellular organisms; Bacteria; PVC group; Planctomycetes; Planctomycetia; Planctomycetales; Planctomycetaceae; Calycomorphotria

Average proteome isoelectric point is 5.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4286 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A517T4J2|A0A517T4J2_9PLAN Inosine-5'-monophosphate dehydrogenase OS=Calycomorphotria hydatis OX=2528027 GN=guaB_1 PE=3 SV=1
MM1 pKa = 5.6TTYY4 pKa = 10.74YY5 pKa = 10.73FSLQLSGIDD14 pKa = 3.48VMDD17 pKa = 3.74NEE19 pKa = 4.84FYY21 pKa = 11.1DD22 pKa = 3.97RR23 pKa = 11.84LAEE26 pKa = 4.07VSAYY30 pKa = 10.67DD31 pKa = 5.47PITKK35 pKa = 9.2EE36 pKa = 3.79QNGVSMISYY45 pKa = 10.0AVEE48 pKa = 4.05ADD50 pKa = 3.73SFDD53 pKa = 3.68SAIRR57 pKa = 11.84AAVKK61 pKa = 9.56EE62 pKa = 4.26VRR64 pKa = 11.84QAAPGCVVDD73 pKa = 5.3QLLVDD78 pKa = 4.63DD79 pKa = 4.46STVTEE84 pKa = 4.26LVEE87 pKa = 4.26GG88 pKa = 3.97

Molecular weight: 9.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A517T3W2|A0A517T3W2_9PLAN Uncharacterized protein OS=Calycomorphotria hydatis OX=2528027 GN=V22_02630 PE=4 SV=1
MM1 pKa = 7.71PSFIRR6 pKa = 11.84IHH8 pKa = 6.14NSSRR12 pKa = 11.84DD13 pKa = 3.41RR14 pKa = 11.84NGADD18 pKa = 2.92RR19 pKa = 11.84VTLPAHH25 pKa = 6.56KK26 pKa = 9.8RR27 pKa = 11.84TDD29 pKa = 3.6NNQSPLLRR37 pKa = 11.84ARR39 pKa = 11.84SARR42 pKa = 11.84FLSRR46 pKa = 11.84LLFSEE51 pKa = 5.37HH52 pKa = 6.3IQPAMCLPNTTKK64 pKa = 10.56AGSALRR70 pKa = 11.84VNGWLNGIPAFAGMTEE86 pKa = 4.14YY87 pKa = 10.72KK88 pKa = 10.55PEE90 pKa = 3.95ACYY93 pKa = 10.18FGAWGLLGASRR104 pKa = 11.84LMRR107 pKa = 11.84GLEE110 pKa = 4.01VVPVEE115 pKa = 4.51SVLLSHH121 pKa = 7.37PPHH124 pKa = 6.81PPEE127 pKa = 4.19PQPGVFFSLMAFQPRR142 pKa = 3.33

Molecular weight: 15.75 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4286

0

4286

1496807

31

5544

349.2

38.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.837 ± 0.034

1.175 ± 0.016

5.925 ± 0.027

7.004 ± 0.04

3.895 ± 0.019

7.532 ± 0.043

2.28 ± 0.02

5.393 ± 0.025

4.007 ± 0.034

9.831 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.221 ± 0.019

3.473 ± 0.027

5.075 ± 0.03

3.908 ± 0.025

6.224 ± 0.034

6.468 ± 0.028

5.638 ± 0.042

7.059 ± 0.029

1.43 ± 0.014

2.627 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski