Sulfitobacter brevis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Roseobacteraceae; Sulfitobacter

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4154 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I1TNU5|A0A1I1TNU5_9RHOB Uncharacterized protein OS=Sulfitobacter brevis OX=74348 GN=SAMN04488523_101414 PE=4 SV=1
MM1 pKa = 7.51TSTGSTYY8 pKa = 10.67DD9 pKa = 2.93ITFQEE14 pKa = 4.47IGSAPRR20 pKa = 11.84DD21 pKa = 3.53LNANWGYY28 pKa = 11.35NEE30 pKa = 4.6LDD32 pKa = 3.62GYY34 pKa = 10.93LYY36 pKa = 9.73TVRR39 pKa = 11.84PGRR42 pKa = 11.84TDD44 pKa = 3.68LYY46 pKa = 10.92RR47 pKa = 11.84VDD49 pKa = 3.6GSGNFVDD56 pKa = 6.75FANLPNTAFDD66 pKa = 3.9GSTAGDD72 pKa = 3.65IFPNGTMVYY81 pKa = 10.37VGDD84 pKa = 4.05ANSWQLIGLATPTAPIDD101 pKa = 4.31LGTLEE106 pKa = 5.35LNQSVNVQDD115 pKa = 3.56IAYY118 pKa = 9.53NPADD122 pKa = 3.52GFLYY126 pKa = 10.45GINRR130 pKa = 11.84NTGRR134 pKa = 11.84AFRR137 pKa = 11.84VDD139 pKa = 3.46GNGGTFGNVTVIEE152 pKa = 4.6FGPAIYY158 pKa = 10.31AGVFASVWFDD168 pKa = 3.15EE169 pKa = 4.55DD170 pKa = 3.46GRR172 pKa = 11.84FYY174 pKa = 10.99GYY176 pKa = 11.39SNTTNNFFLIDD187 pKa = 3.82TTTGNAVPIANGAVDD202 pKa = 4.06EE203 pKa = 5.34GGDD206 pKa = 3.87SDD208 pKa = 4.51GASCRR213 pKa = 11.84GPAPISFGSISGSVYY228 pKa = 11.04DD229 pKa = 5.19DD230 pKa = 4.74LNGSDD235 pKa = 3.45VRR237 pKa = 11.84EE238 pKa = 4.34AGEE241 pKa = 4.0TALGAGIRR249 pKa = 11.84IDD251 pKa = 4.51LYY253 pKa = 11.05YY254 pKa = 11.2DD255 pKa = 3.19NGTPSNYY262 pKa = 9.29TDD264 pKa = 4.73DD265 pKa = 4.9VFAQTTDD272 pKa = 3.3TLSDD276 pKa = 3.38GTYY279 pKa = 9.38TFSDD283 pKa = 3.61LVINQSYY290 pKa = 10.31RR291 pKa = 11.84IQLDD295 pKa = 3.43EE296 pKa = 5.28ADD298 pKa = 4.49PDD300 pKa = 4.0LGPGNLIGTSNPILGVSVAAGAITADD326 pKa = 3.41QDD328 pKa = 3.96FGFDD332 pKa = 3.68PSGSDD337 pKa = 3.94LSLSKK342 pKa = 10.79YY343 pKa = 10.1AAATGTTTPITAVVEE358 pKa = 4.0GDD360 pKa = 3.6TIDD363 pKa = 3.36WVVTITNSGSGSPSGVKK380 pKa = 10.44VIDD383 pKa = 5.25LIPSGFAYY391 pKa = 10.75VSDD394 pKa = 4.63DD395 pKa = 3.77APATGDD401 pKa = 3.71SYY403 pKa = 12.04DD404 pKa = 3.91PSTGLWFVDD413 pKa = 4.9EE414 pKa = 4.53ILSGTSEE421 pKa = 4.38TLTITTTVLGSGDD434 pKa = 3.44FTNYY438 pKa = 10.86AEE440 pKa = 5.07IIYY443 pKa = 10.51SSLPDD448 pKa = 4.5PDD450 pKa = 4.78SDD452 pKa = 4.36PDD454 pKa = 3.68TGRR457 pKa = 11.84LTDD460 pKa = 4.86DD461 pKa = 4.7LSDD464 pKa = 4.17QIGDD468 pKa = 4.05DD469 pKa = 4.45DD470 pKa = 4.05EE471 pKa = 6.8ASYY474 pKa = 11.38AVALQTGSRR483 pKa = 11.84LLSGRR488 pKa = 11.84LFVDD492 pKa = 3.56NGRR495 pKa = 11.84GGGTAHH501 pKa = 7.42DD502 pKa = 4.49GEE504 pKa = 4.79INGAEE509 pKa = 4.23AGSKK513 pKa = 9.77SAQLQILDD521 pKa = 4.01SGGAEE526 pKa = 3.37IGAPQVAADD535 pKa = 4.47GSWSFALSGSYY546 pKa = 10.0SGPLTVRR553 pKa = 11.84ATPLSSHH560 pKa = 5.46ITISEE565 pKa = 3.93ATAGLDD571 pKa = 3.25ALVNADD577 pKa = 3.48PHH579 pKa = 7.89DD580 pKa = 4.27GEE582 pKa = 4.8FTFTPEE588 pKa = 3.97PFGHH592 pKa = 7.01KK593 pKa = 10.01SGLNIGLLEE602 pKa = 4.36TPLLTWNRR610 pKa = 11.84QATVGRR616 pKa = 11.84GQVAEE621 pKa = 4.22LQHH624 pKa = 6.93IYY626 pKa = 9.47TATSQSNVSFTFQNVDD642 pKa = 3.47SAPANAFNASLFLDD656 pKa = 4.15SDD658 pKa = 4.48CDD660 pKa = 3.56GHH662 pKa = 8.09SDD664 pKa = 3.2ITITSPIGVVPEE676 pKa = 3.85QQLCIISRR684 pKa = 11.84VTAASGAGQGSSYY697 pKa = 11.02VYY699 pKa = 10.52QLVAATAFNGTTKK712 pKa = 10.27VHH714 pKa = 5.45TTLNTDD720 pKa = 3.37QVSVTSGTGNVEE732 pKa = 3.65LSKK735 pKa = 9.84TVEE738 pKa = 4.0NQTIGSPEE746 pKa = 3.92GTSNLGRR753 pKa = 11.84AGDD756 pKa = 3.46ILTYY760 pKa = 10.26RR761 pKa = 11.84IYY763 pKa = 11.23LLNTSPQPISNVKK776 pKa = 10.2VYY778 pKa = 11.04DD779 pKa = 3.36RR780 pKa = 11.84TPPYY784 pKa = 9.64TSLSAPIADD793 pKa = 4.03PVTVSPNLTCTVAKK807 pKa = 9.99PVANVTGYY815 pKa = 10.67EE816 pKa = 4.23GDD818 pKa = 4.68LEE820 pKa = 4.65WTCSGSFLPGEE831 pKa = 4.17TGAVEE836 pKa = 4.21FSVAISPP843 pKa = 4.28

Molecular weight: 87.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I1TQP8|A0A1I1TQP8_9RHOB L-glutamine ABC transporter membrane protein /L-glutamate ABC transporter membrane protein /L-aspartate ABC transporter membrane protein /L-asparagine ABC transporter membrane protein OS=Sulfitobacter brevis OX=74348 GN=SAMN04488523_101435 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37AGRR28 pKa = 11.84KK29 pKa = 8.81IINARR34 pKa = 11.84RR35 pKa = 11.84AQGRR39 pKa = 11.84KK40 pKa = 8.52EE41 pKa = 3.76LSAA44 pKa = 4.93

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4154

0

4154

1253621

40

2159

301.8

32.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.139 ± 0.045

0.907 ± 0.011

6.012 ± 0.031

5.666 ± 0.033

3.717 ± 0.024

8.477 ± 0.042

2.081 ± 0.019

5.391 ± 0.024

3.47 ± 0.031

9.918 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.915 ± 0.021

2.803 ± 0.021

4.903 ± 0.03

3.379 ± 0.02

6.334 ± 0.038

5.363 ± 0.025

5.666 ± 0.025

7.264 ± 0.033

1.368 ± 0.016

2.224 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski