Hubei lepidoptera virus 3

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 5.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1L3KNZ0|A0A1L3KNZ0_9VIRU Cypovirus VP8 OS=Hubei lepidoptera virus 3 OX=1922905 PE=4 SV=1
MM1 pKa = 7.41LTFQVTFKK9 pKa = 10.87DD10 pKa = 3.83VKK12 pKa = 10.43TNDD15 pKa = 3.16LKK17 pKa = 11.17KK18 pKa = 10.71INIHH22 pKa = 4.61TAKK25 pKa = 9.72PQKK28 pKa = 10.72DD29 pKa = 3.32YY30 pKa = 11.81DD31 pKa = 3.84KK32 pKa = 11.45FHH34 pKa = 7.89DD35 pKa = 4.44KK36 pKa = 10.45LAKK39 pKa = 9.84KK40 pKa = 10.21NRR42 pKa = 11.84EE43 pKa = 3.79LTQNAKK49 pKa = 9.26MSIMPRR55 pKa = 11.84VVTDD59 pKa = 3.63KK60 pKa = 10.32VTADD64 pKa = 4.98SIIQKK69 pKa = 10.34NKK71 pKa = 9.71IEE73 pKa = 4.16TEE75 pKa = 3.89VLYY78 pKa = 10.53ISMRR82 pKa = 11.84APRR85 pKa = 11.84IDD87 pKa = 3.75PDD89 pKa = 4.77AIVCANEE96 pKa = 3.86LGLPILSRR104 pKa = 11.84VGVEE108 pKa = 3.45EE109 pKa = 4.53TMFLKK114 pKa = 10.51DD115 pKa = 3.3KK116 pKa = 10.43RR117 pKa = 11.84YY118 pKa = 9.3QYY120 pKa = 10.98VSKK123 pKa = 11.22EE124 pKa = 3.99EE125 pKa = 3.95VDD127 pKa = 3.37VDD129 pKa = 4.7FFDD132 pKa = 4.32EE133 pKa = 4.05LTMAINRR140 pKa = 11.84KK141 pKa = 8.95IGKK144 pKa = 9.69KK145 pKa = 9.87SGSDD149 pKa = 3.35EE150 pKa = 3.73GTMIKK155 pKa = 9.31MVKK158 pKa = 10.01NDD160 pKa = 4.13LKK162 pKa = 9.73SASWIMEE169 pKa = 4.08NNVKK173 pKa = 10.62LFVRR177 pKa = 11.84TGLNKK182 pKa = 10.41KK183 pKa = 10.38LDD185 pKa = 4.24DD186 pKa = 4.36LCVKK190 pKa = 10.35PSDD193 pKa = 3.31IGNVRR198 pKa = 11.84EE199 pKa = 3.8IRR201 pKa = 11.84AVDD204 pKa = 3.87EE205 pKa = 4.29RR206 pKa = 11.84WHH208 pKa = 7.45GEE210 pKa = 4.09DD211 pKa = 3.33EE212 pKa = 4.76GVTLTYY218 pKa = 10.48SIYY221 pKa = 11.16SNDD224 pKa = 3.19FNEE227 pKa = 5.4LIRR230 pKa = 11.84TQEE233 pKa = 3.22IDD235 pKa = 3.03KK236 pKa = 10.82AIRR239 pKa = 11.84DD240 pKa = 4.02VKK242 pKa = 10.26EE243 pKa = 4.31HH244 pKa = 7.18IEE246 pKa = 4.31DD247 pKa = 3.46EE248 pKa = 4.17HH249 pKa = 8.4HH250 pKa = 5.75GAKK253 pKa = 9.52GRR255 pKa = 11.84KK256 pKa = 9.07VYY258 pKa = 10.49IIYY261 pKa = 10.65DD262 pKa = 3.8GEE264 pKa = 4.56DD265 pKa = 3.41SNKK268 pKa = 9.62MKK270 pKa = 10.58VKK272 pKa = 10.63KK273 pKa = 10.24FINEE277 pKa = 4.0LSYY280 pKa = 10.99EE281 pKa = 3.77IDD283 pKa = 3.68GQVVEE288 pKa = 4.28YY289 pKa = 10.32RR290 pKa = 11.84VNEE293 pKa = 4.13KK294 pKa = 10.99NDD296 pKa = 3.32VKK298 pKa = 11.18YY299 pKa = 11.19SLIQTCRR306 pKa = 11.84IAEE309 pKa = 4.19SEE311 pKa = 4.64LEE313 pKa = 4.22DD314 pKa = 3.55EE315 pKa = 4.48TMKK318 pKa = 11.33DD319 pKa = 3.23NVIFEE324 pKa = 4.56GKK326 pKa = 8.44RR327 pKa = 11.84TEE329 pKa = 4.03LKK331 pKa = 10.57EE332 pKa = 4.06IILNEE337 pKa = 3.8IEE339 pKa = 4.01PLYY342 pKa = 10.79RR343 pKa = 11.84KK344 pKa = 9.97APEE347 pKa = 4.11SGRR350 pKa = 11.84EE351 pKa = 4.28DD352 pKa = 3.5IDD354 pKa = 4.27KK355 pKa = 10.6MKK357 pKa = 10.99ALLNSEE363 pKa = 5.1RR364 pKa = 11.84YY365 pKa = 10.25VNDD368 pKa = 3.15TDD370 pKa = 3.94VIKK373 pKa = 10.92VEE375 pKa = 5.0RR376 pKa = 11.84IPLPSKK382 pKa = 10.44KK383 pKa = 9.95PEE385 pKa = 3.51IAEE388 pKa = 4.22NVNKK392 pKa = 9.11MRR394 pKa = 11.84KK395 pKa = 9.7DD396 pKa = 3.19ILVKK400 pKa = 10.34IGKK403 pKa = 9.63CDD405 pKa = 3.73DD406 pKa = 4.23AKK408 pKa = 11.18DD409 pKa = 3.58GAIIMSLEE417 pKa = 3.96RR418 pKa = 11.84EE419 pKa = 4.11LGNNIWRR426 pKa = 11.84GEE428 pKa = 4.18EE429 pKa = 3.75TWQSRR434 pKa = 11.84MQKK437 pKa = 9.69RR438 pKa = 11.84FPEE441 pKa = 4.14TSVFYY446 pKa = 10.27AYY448 pKa = 10.48SDD450 pKa = 3.48RR451 pKa = 11.84VGTGDD456 pKa = 4.57GIKK459 pKa = 9.41IDD461 pKa = 3.7KK462 pKa = 10.35AIKK465 pKa = 10.45NKK467 pKa = 8.68MSKK470 pKa = 10.04PKK472 pKa = 10.24SVYY475 pKa = 10.36NKK477 pKa = 10.12EE478 pKa = 4.25DD479 pKa = 3.42LEE481 pKa = 4.47KK482 pKa = 11.3YY483 pKa = 9.7EE484 pKa = 5.02IIGIEE489 pKa = 3.94PNYY492 pKa = 10.35VEE494 pKa = 4.54SLEE497 pKa = 4.39EE498 pKa = 3.73EE499 pKa = 4.11LDD501 pKa = 3.57RR502 pKa = 11.84RR503 pKa = 11.84NVDD506 pKa = 3.31LEE508 pKa = 3.94LRR510 pKa = 11.84KK511 pKa = 10.13LISAYY516 pKa = 10.63SSAKK520 pKa = 9.94NRR522 pKa = 11.84MILTEE527 pKa = 4.63MIACEE532 pKa = 4.13NYY534 pKa = 10.43NGNTFIKK541 pKa = 10.33FIEE544 pKa = 4.05QMIAASVADD553 pKa = 3.74KK554 pKa = 10.83GITMFVEE561 pKa = 4.47DD562 pKa = 4.73EE563 pKa = 4.38SFKK566 pKa = 11.03TMADD570 pKa = 3.06KK571 pKa = 11.02VINKK575 pKa = 8.62IRR577 pKa = 11.84NEE579 pKa = 3.73NMEE582 pKa = 3.75IRR584 pKa = 11.84EE585 pKa = 4.19VYY587 pKa = 10.35QLFITEE593 pKa = 4.49KK594 pKa = 10.05YY595 pKa = 9.83INARR599 pKa = 11.84DD600 pKa = 3.73FLGEE604 pKa = 4.05SGDD607 pKa = 3.71IASEE611 pKa = 4.03VAEE614 pKa = 4.76DD615 pKa = 4.42KK616 pKa = 10.84KK617 pKa = 10.95DD618 pKa = 3.52SQIFEE623 pKa = 4.26EE624 pKa = 4.63EE625 pKa = 4.37GNDD628 pKa = 3.37EE629 pKa = 4.32YY630 pKa = 11.41EE631 pKa = 4.14EE632 pKa = 3.92EE633 pKa = 4.25RR634 pKa = 11.84NLVKK638 pKa = 10.47EE639 pKa = 4.2DD640 pKa = 3.6NGNEE644 pKa = 4.04EE645 pKa = 4.31LSDD648 pKa = 3.6VRR650 pKa = 11.84DD651 pKa = 3.46KK652 pKa = 11.54KK653 pKa = 10.74EE654 pKa = 3.89EE655 pKa = 4.03KK656 pKa = 10.35EE657 pKa = 3.9EE658 pKa = 4.57SNGGKK663 pKa = 9.25VRR665 pKa = 11.84YY666 pKa = 9.26EE667 pKa = 3.79SVIVNEE673 pKa = 4.6KK674 pKa = 10.73NDD676 pKa = 3.65DD677 pKa = 3.78KK678 pKa = 11.51NFVVIDD684 pKa = 3.78VYY686 pKa = 11.43EE687 pKa = 4.31EE688 pKa = 4.21GKK690 pKa = 11.18KK691 pKa = 9.51MLTDD695 pKa = 3.84AFNRR699 pKa = 11.84SFEE702 pKa = 4.26EE703 pKa = 3.81LRR705 pKa = 11.84EE706 pKa = 4.26AKK708 pKa = 9.5TKK710 pKa = 9.62EE711 pKa = 3.58QIEE714 pKa = 4.4IIKK717 pKa = 10.15LKK719 pKa = 10.79SEE721 pKa = 4.37TYY723 pKa = 10.05VEE725 pKa = 4.31LFKK728 pKa = 11.15IMNRR732 pKa = 11.84GMGEE736 pKa = 3.75LEE738 pKa = 4.6EE739 pKa = 4.06IRR741 pKa = 11.84KK742 pKa = 9.75EE743 pKa = 4.03NVNKK747 pKa = 9.96VEE749 pKa = 4.08RR750 pKa = 11.84VEE752 pKa = 4.8EE753 pKa = 3.94FDD755 pKa = 5.54KK756 pKa = 11.57GDD758 pKa = 3.45NTDD761 pKa = 3.28NRR763 pKa = 11.84VVEE766 pKa = 4.35MKK768 pKa = 10.41EE769 pKa = 3.95KK770 pKa = 10.66VVNALMLTHH779 pKa = 7.02IDD781 pKa = 3.55FMEE784 pKa = 4.33KK785 pKa = 10.27VEE787 pKa = 4.03MDD789 pKa = 3.7EE790 pKa = 4.97RR791 pKa = 11.84PIIMVARR798 pKa = 11.84SEE800 pKa = 4.34KK801 pKa = 9.66YY802 pKa = 9.8SGYY805 pKa = 5.45EE806 pKa = 3.71TKK808 pKa = 10.16MKK810 pKa = 9.73GTIRR814 pKa = 11.84YY815 pKa = 9.9GEE817 pKa = 4.24TNDD820 pKa = 3.06RR821 pKa = 11.84KK822 pKa = 10.81YY823 pKa = 11.44NVFEE827 pKa = 4.67SNTIKK832 pKa = 10.67KK833 pKa = 9.84VIQDD837 pKa = 3.49EE838 pKa = 4.32MRR840 pKa = 11.84SGGNEE845 pKa = 3.65RR846 pKa = 11.84ATTEE850 pKa = 3.91AVLNMLKK857 pKa = 10.13ISLHH861 pKa = 4.92KK862 pKa = 10.04RR863 pKa = 11.84GYY865 pKa = 8.96VVEE868 pKa = 4.31TNEE871 pKa = 4.21EE872 pKa = 3.52GDD874 pKa = 3.65IIIRR878 pKa = 11.84KK879 pKa = 7.87YY880 pKa = 10.78

Molecular weight:
102.36 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1L3KP70|A0A1L3KP70_9VIRU Cypovirus VP2 OS=Hubei lepidoptera virus 3 OX=1922905 PE=4 SV=1
MM1 pKa = 7.72EE2 pKa = 5.08KK3 pKa = 10.3RR4 pKa = 11.84VINKK8 pKa = 9.26NVINKK13 pKa = 9.3EE14 pKa = 3.63KK15 pKa = 10.46EE16 pKa = 3.7INKK19 pKa = 9.79KK20 pKa = 10.11YY21 pKa = 10.2KK22 pKa = 9.71PKK24 pKa = 10.62NRR26 pKa = 11.84LNVIWTVNLKK36 pKa = 10.37DD37 pKa = 3.9KK38 pKa = 11.09NVLRR42 pKa = 11.84ILSEE46 pKa = 4.18LGVEE50 pKa = 4.86IYY52 pKa = 10.37RR53 pKa = 11.84QEE55 pKa = 4.2GEE57 pKa = 4.18KK58 pKa = 10.77KK59 pKa = 9.04SDD61 pKa = 2.76RR62 pKa = 11.84RR63 pKa = 11.84ARR65 pKa = 11.84AICANEE71 pKa = 3.95QIEE74 pKa = 4.49WASDD78 pKa = 2.88IHH80 pKa = 6.45GWLDD84 pKa = 3.63KK85 pKa = 10.93LSKK88 pKa = 10.63RR89 pKa = 11.84NNEE92 pKa = 3.67NLMNLGVISKK102 pKa = 9.98ILRR105 pKa = 11.84NEE107 pKa = 3.91VIEE110 pKa = 4.29YY111 pKa = 10.04NVSEE115 pKa = 4.25GQLKK119 pKa = 10.23RR120 pKa = 11.84EE121 pKa = 3.98EE122 pKa = 4.22TKK124 pKa = 10.52EE125 pKa = 3.8IEE127 pKa = 4.33VEE129 pKa = 4.19SEE131 pKa = 4.06GLLKK135 pKa = 10.77YY136 pKa = 10.63NRR138 pKa = 11.84ADD140 pKa = 3.4NDD142 pKa = 3.41NVMKK146 pKa = 9.44LTKK149 pKa = 10.55EE150 pKa = 3.9EE151 pKa = 4.11IEE153 pKa = 4.41CLKK156 pKa = 9.89EE157 pKa = 4.0TIKK160 pKa = 10.15TGLHH164 pKa = 5.55SEE166 pKa = 4.32GAAFKK171 pKa = 9.94MIEE174 pKa = 4.02IKK176 pKa = 10.48IPALMRR182 pKa = 11.84LTPKK186 pKa = 10.57YY187 pKa = 10.06INQLRR192 pKa = 11.84ARR194 pKa = 11.84VNVEE198 pKa = 3.83QISVTEE204 pKa = 4.87IIHH207 pKa = 6.15SDD209 pKa = 2.85NYY211 pKa = 7.11YY212 pKa = 11.16ARR214 pKa = 11.84MIFEE218 pKa = 4.83KK219 pKa = 10.66PLLIYY224 pKa = 10.47NAISMNVIRR233 pKa = 11.84KK234 pKa = 9.26LKK236 pKa = 10.56ADD238 pKa = 3.77DD239 pKa = 3.56RR240 pKa = 11.84QIYY243 pKa = 9.82IMSGEE248 pKa = 4.21VNHH251 pKa = 7.27KK252 pKa = 10.62DD253 pKa = 3.39MRR255 pKa = 11.84DD256 pKa = 3.06KK257 pKa = 11.14GLRR260 pKa = 11.84VYY262 pKa = 10.55VPQASKK268 pKa = 11.26LIFTMKK274 pKa = 9.99NRR276 pKa = 11.84KK277 pKa = 8.99LSIYY281 pKa = 10.43VGLPPQMFQNRR292 pKa = 11.84IDD294 pKa = 3.63EE295 pKa = 4.71RR296 pKa = 11.84YY297 pKa = 10.02HH298 pKa = 6.53NFTNEE303 pKa = 3.52IADD306 pKa = 3.34ITMIPSMQSTFGMGMSKK323 pKa = 9.01LTRR326 pKa = 11.84VMAATPSTPRR336 pKa = 11.84LTSKK340 pKa = 9.91ATLEE344 pKa = 3.99KK345 pKa = 10.71LKK347 pKa = 10.45MEE349 pKa = 4.43IASDD353 pKa = 3.76RR354 pKa = 11.84KK355 pKa = 9.83VVTCIVIGNKK365 pKa = 10.22GSGKK369 pKa = 6.85TTMTKK374 pKa = 10.72KK375 pKa = 9.8MVEE378 pKa = 4.53LLDD381 pKa = 3.73GMNGRR386 pKa = 11.84RR387 pKa = 11.84CYY389 pKa = 10.53RR390 pKa = 11.84IDD392 pKa = 3.18SDD394 pKa = 4.99APGRR398 pKa = 11.84WMYY401 pKa = 11.56DD402 pKa = 2.79RR403 pKa = 11.84ANKK406 pKa = 8.81VVCTSFDD413 pKa = 3.25EE414 pKa = 4.39VLSYY418 pKa = 11.06NSEE421 pKa = 4.3MYY423 pKa = 10.2ISLYY427 pKa = 10.87EE428 pKa = 4.09KK429 pKa = 10.22MVDD432 pKa = 5.09DD433 pKa = 4.14MMQAEE438 pKa = 4.83KK439 pKa = 11.12LDD441 pKa = 3.51VDD443 pKa = 3.77KK444 pKa = 11.01FNRR447 pKa = 11.84MSNAKK452 pKa = 9.32KK453 pKa = 10.67NEE455 pKa = 4.04MIDD458 pKa = 3.26ALKK461 pKa = 11.0RR462 pKa = 11.84MIDD465 pKa = 4.14DD466 pKa = 3.85KK467 pKa = 11.46LISNKK472 pKa = 10.31EE473 pKa = 3.98EE474 pKa = 3.89VNEE477 pKa = 3.99KK478 pKa = 10.19QFYY481 pKa = 11.18DD482 pKa = 3.72MVHH485 pKa = 6.19EE486 pKa = 4.14LVPVNSTLIIEE497 pKa = 4.54AHH499 pKa = 7.4RR500 pKa = 11.84ITQDD504 pKa = 2.94AVLGGTDD511 pKa = 2.86ISMLYY516 pKa = 10.41EE517 pKa = 4.68GVQDD521 pKa = 3.79PLIAFMRR528 pKa = 11.84RR529 pKa = 11.84GALLRR534 pKa = 11.84DD535 pKa = 4.31LILRR539 pKa = 11.84EE540 pKa = 4.49IYY542 pKa = 10.45QKK544 pKa = 10.73DD545 pKa = 3.63YY546 pKa = 11.07SYY548 pKa = 11.56SHH550 pKa = 6.88VMINAMEE557 pKa = 4.16MGVVLSEE564 pKa = 4.07

Molecular weight:
65.39 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10

0

10

7784

253

1335

778.4

89.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.959 ± 0.327

0.732 ± 0.23

6.115 ± 0.249

8.941 ± 0.695

3.25 ± 0.2

5.588 ± 0.395

1.375 ± 0.11

8.363 ± 0.338

7.027 ± 0.878

7.798 ± 0.363

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

4.188 ± 0.218

6.385 ± 0.336

2.595 ± 0.198

2.762 ± 0.287

6.655 ± 0.258

6.166 ± 0.365

5.383 ± 0.393

6.706 ± 0.361

0.797 ± 0.086

4.214 ± 0.212

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski