Mycobacterium phage ACFishhook

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fromanvirus; unclassified Fromanvirus

Average proteome isoelectric point is 6.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 77 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3Q9R8H5|A0A3Q9R8H5_9CAUD Uncharacterized protein OS=Mycobacterium phage ACFishhook OX=2500573 GN=6 PE=4 SV=1
MM1 pKa = 7.74NDD3 pKa = 3.6PEE5 pKa = 4.12QLALFDD11 pKa = 3.88VDD13 pKa = 3.63EE14 pKa = 5.19LGWTEE19 pKa = 4.08SVHH22 pKa = 7.58DD23 pKa = 4.28YY24 pKa = 10.62VHH26 pKa = 6.77QGDD29 pKa = 3.98EE30 pKa = 3.96

Molecular weight: 3.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3Q9R8J2|A0A3Q9R8J2_9CAUD DNA primase OS=Mycobacterium phage ACFishhook OX=2500573 GN=58 PE=4 SV=1
MM1 pKa = 6.62TVKK4 pKa = 10.65VNGRR8 pKa = 11.84KK9 pKa = 9.46LEE11 pKa = 4.51PGTEE15 pKa = 3.96VSIKK19 pKa = 10.24GEE21 pKa = 3.72RR22 pKa = 11.84GRR24 pKa = 11.84YY25 pKa = 8.8RR26 pKa = 11.84FVKK29 pKa = 9.76ATTTSQGKK37 pKa = 7.25TVLDD41 pKa = 4.81FIGGPAGHH49 pKa = 5.74EE50 pKa = 4.16QWRR53 pKa = 11.84SFYY56 pKa = 9.51PEE58 pKa = 3.66RR59 pKa = 11.84VEE61 pKa = 4.27TVHH64 pKa = 7.27RR65 pKa = 11.84IARR68 pKa = 11.84TRR70 pKa = 11.84ANAKK74 pKa = 9.8

Molecular weight: 8.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

77

0

77

14471

20

1008

187.9

20.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.84 ± 0.384

0.663 ± 0.097

6.309 ± 0.234

6.765 ± 0.334

3.393 ± 0.221

8.631 ± 0.599

1.99 ± 0.187

4.685 ± 0.187

4.319 ± 0.208

8.216 ± 0.338

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.598 ± 0.216

3.269 ± 0.187

5.66 ± 0.329

3.911 ± 0.326

6.731 ± 0.376

5.065 ± 0.204

6.15 ± 0.239

6.966 ± 0.222

1.907 ± 0.151

2.93 ± 0.195
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski