Apis mellifera associated microvirus 13

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 6.99

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3Q8U5P9|A0A3Q8U5P9_9VIRU Nonstructural protein OS=Apis mellifera associated microvirus 13 OX=2494740 PE=4 SV=1
MM1 pKa = 7.87AKK3 pKa = 10.34LQMLNAEE10 pKa = 4.2TGEE13 pKa = 4.36IIDD16 pKa = 4.26NNHH19 pKa = 5.89PFIRR23 pKa = 11.84TPYY26 pKa = 10.43NYY28 pKa = 10.45DD29 pKa = 3.01RR30 pKa = 11.84DD31 pKa = 3.93AASLEE36 pKa = 4.23TATVCTEE43 pKa = 3.65EE44 pKa = 4.55TLAQQQFKK52 pKa = 10.78DD53 pKa = 3.44EE54 pKa = 4.42CDD56 pKa = 2.95INTILEE62 pKa = 4.19RR63 pKa = 11.84FGITGEE69 pKa = 4.07LPQNLRR75 pKa = 11.84QPLSADD81 pKa = 3.94FIEE84 pKa = 5.47AYY86 pKa = 10.03DD87 pKa = 3.96YY88 pKa = 9.01KK89 pKa = 10.77TAMDD93 pKa = 5.08SIIEE97 pKa = 4.05AQNAFMEE104 pKa = 4.65LPAKK108 pKa = 10.17IRR110 pKa = 11.84EE111 pKa = 4.24EE112 pKa = 4.17FNNDD116 pKa = 3.1PGQFIQFFEE125 pKa = 4.3QEE127 pKa = 4.2EE128 pKa = 4.04NRR130 pKa = 11.84ARR132 pKa = 11.84AIEE135 pKa = 4.36LGLIQAPRR143 pKa = 11.84EE144 pKa = 4.09PANQAPADD152 pKa = 4.0PTPPTTT158 pKa = 4.07

Molecular weight: 17.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3S8UTU0|A0A3S8UTU0_9VIRU Replication initiator protein OS=Apis mellifera associated microvirus 13 OX=2494740 PE=4 SV=1
MM1 pKa = 7.26EE2 pKa = 6.08ALMPCYY8 pKa = 10.18HH9 pKa = 7.73PINAYY14 pKa = 7.94KK15 pKa = 8.96TASGEE20 pKa = 4.04IVFTEE25 pKa = 4.05KK26 pKa = 10.61ARR28 pKa = 11.84YY29 pKa = 9.9GDD31 pKa = 3.65TQHH34 pKa = 6.96IKK36 pKa = 10.47LPCGQCIGCRR46 pKa = 11.84LEE48 pKa = 5.65RR49 pKa = 11.84SRR51 pKa = 11.84QWGVRR56 pKa = 11.84CMHH59 pKa = 7.17EE60 pKa = 4.07AQMHH64 pKa = 5.16KK65 pKa = 10.46QNSFVTLTYY74 pKa = 10.37NAEE77 pKa = 4.01NTPQRR82 pKa = 11.84GNLNHH87 pKa = 7.45ADD89 pKa = 3.51FQKK92 pKa = 10.67FMKK95 pKa = 10.4RR96 pKa = 11.84LRR98 pKa = 11.84KK99 pKa = 8.8HH100 pKa = 5.99AKK102 pKa = 8.25TDD104 pKa = 2.53IRR106 pKa = 11.84YY107 pKa = 10.01YY108 pKa = 10.15MGGEE112 pKa = 4.03YY113 pKa = 10.76GPTTWRR119 pKa = 11.84PHH121 pKa = 4.32FHH123 pKa = 6.81ACIFGYY129 pKa = 10.77DD130 pKa = 3.85FADD133 pKa = 3.07KK134 pKa = 10.86LYY136 pKa = 10.54FKK138 pKa = 10.12TGEE141 pKa = 4.2SGEE144 pKa = 4.4KK145 pKa = 9.68IYY147 pKa = 10.5TSKK150 pKa = 9.59TLEE153 pKa = 4.14KK154 pKa = 9.37LWPYY158 pKa = 10.81GFSTIGNVTFQSAAYY173 pKa = 8.08IARR176 pKa = 11.84YY177 pKa = 8.78CVQKK181 pKa = 9.95VTGQAAEE188 pKa = 3.9EE189 pKa = 4.41HH190 pKa = 6.46YY191 pKa = 11.01KK192 pKa = 10.66RR193 pKa = 11.84VDD195 pKa = 3.41DD196 pKa = 3.71QGEE199 pKa = 4.17YY200 pKa = 10.74QLTPEE205 pKa = 4.39YY206 pKa = 11.26NRR208 pKa = 11.84MSLKK212 pKa = 10.3PAIGKK217 pKa = 7.49TWLEE221 pKa = 4.24KK222 pKa = 10.68YY223 pKa = 10.47RR224 pKa = 11.84SDD226 pKa = 4.75VYY228 pKa = 11.53NHH230 pKa = 6.51DD231 pKa = 3.55HH232 pKa = 6.81VIINGVKK239 pKa = 8.56STPPKK244 pKa = 10.88YY245 pKa = 10.09YY246 pKa = 10.67DD247 pKa = 3.69KK248 pKa = 11.19LLKK251 pKa = 10.47RR252 pKa = 11.84WDD254 pKa = 3.59ANRR257 pKa = 11.84LEE259 pKa = 4.1EE260 pKa = 5.04LKK262 pKa = 10.56EE263 pKa = 3.81QRR265 pKa = 11.84EE266 pKa = 4.51ANAQTHH272 pKa = 6.48KK273 pKa = 10.84EE274 pKa = 4.01DD275 pKa = 3.67QTWQRR280 pKa = 11.84LEE282 pKa = 4.05AKK284 pKa = 9.86EE285 pKa = 4.0QFQHH289 pKa = 6.81ARR291 pKa = 11.84IKK293 pKa = 10.79SLLRR297 pKa = 11.84NKK299 pKa = 9.73II300 pKa = 3.43

Molecular weight: 35.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1428

67

539

238.0

26.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.294 ± 1.383

0.77 ± 0.464

4.692 ± 0.607

5.882 ± 1.352

3.992 ± 0.938

6.162 ± 0.652

2.171 ± 0.801

5.882 ± 0.609

4.902 ± 1.404

7.143 ± 0.527

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.591 ± 0.167

5.602 ± 0.555

5.112 ± 1.069

6.793 ± 0.839

5.602 ± 0.363

4.972 ± 0.937

8.333 ± 0.62

3.852 ± 0.682

1.331 ± 0.382

3.922 ± 0.944
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski