Escherichia phage JN01

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Ounavirinae; Felixounavirus; unclassified Felixounavirus

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 97 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6B9SNY7|A0A6B9SNY7_9CAUD Uncharacterized protein OS=Escherichia phage JN01 OX=2692737 PE=4 SV=1
MM1 pKa = 7.47KK2 pKa = 9.8CFHH5 pKa = 6.61GTTQEE10 pKa = 3.93NFINLINNGDD20 pKa = 3.94KK21 pKa = 10.86PSGAWNCSDD30 pKa = 3.4MDD32 pKa = 4.16GNFYY36 pKa = 10.85VYY38 pKa = 10.1PEE40 pKa = 3.92NKK42 pKa = 9.54IYY44 pKa = 10.85GDD46 pKa = 3.97DD47 pKa = 3.63EE48 pKa = 4.51EE49 pKa = 5.5EE50 pKa = 4.57IISEE54 pKa = 5.02GIQQALGNATITAAFQMKK72 pKa = 7.87TQNIVILEE80 pKa = 4.02LDD82 pKa = 3.41IPEE85 pKa = 5.73DD86 pKa = 4.11EE87 pKa = 5.46LNDD90 pKa = 5.32DD91 pKa = 4.11YY92 pKa = 12.12SCDD95 pKa = 3.5NMSSVASFTEE105 pKa = 4.09YY106 pKa = 10.97FDD108 pKa = 5.33LNWIKK113 pKa = 10.48KK114 pKa = 9.52VYY116 pKa = 8.54VTEE119 pKa = 4.72FNAMFSPFCLPSLDD133 pKa = 4.18NPNLNYY139 pKa = 10.59LEE141 pKa = 4.25EE142 pKa = 4.19TLEE145 pKa = 4.14LLAKK149 pKa = 10.12SVQQSDD155 pKa = 4.51SIQVFCDD162 pKa = 3.04IMDD165 pKa = 4.29TLTGNITEE173 pKa = 4.49EE174 pKa = 4.13DD175 pKa = 3.63LKK177 pKa = 11.6SFLL180 pKa = 4.67

Molecular weight: 20.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6B9SP31|A0A6B9SP31_9CAUD Uncharacterized protein OS=Escherichia phage JN01 OX=2692737 PE=4 SV=1
MM1 pKa = 7.97LFRR4 pKa = 11.84LPFKK8 pKa = 10.22KK9 pKa = 8.99TFSEE13 pKa = 4.38PEE15 pKa = 3.78RR16 pKa = 11.84DD17 pKa = 3.57EE18 pKa = 5.39LSISLFILSNNFCLRR33 pKa = 11.84FLIVSFEE40 pKa = 4.08APFIIAAWVLPRR52 pKa = 11.84WLIVNSQYY60 pKa = 11.67LHH62 pKa = 6.16AHH64 pKa = 6.76LLSSLSYY71 pKa = 10.83LLRR74 pKa = 11.84FFPPAIKK81 pKa = 10.08ISTLRR86 pKa = 11.84RR87 pKa = 11.84TSGFTFKK94 pKa = 10.62FLQLLQRR101 pKa = 11.84TINIPSISFF110 pKa = 3.34

Molecular weight: 12.94 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

97

0

97

19995

74

909

206.1

23.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.932 ± 0.319

1.31 ± 0.135

6.022 ± 0.181

6.472 ± 0.291

4.301 ± 0.169

6.377 ± 0.263

1.925 ± 0.184

6.142 ± 0.176

7.497 ± 0.333

8.062 ± 0.178

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.686 ± 0.164

5.561 ± 0.287

3.041 ± 0.159

3.566 ± 0.188

4.171 ± 0.159

6.867 ± 0.231

6.552 ± 0.311

7.232 ± 0.233

1.12 ± 0.106

4.166 ± 0.156
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski