Clostridioides difficile (strain 630) (Peptoclostridium difficile)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Peptostreptococcaceae; Clostridioides; Clostridioides difficile

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3762 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q180N9|Q180N9_CLOD6 Uncharacterized protein OS=Clostridioides difficile (strain 630) OX=272563 GN=CD630_33911 PE=4 SV=1
MM1 pKa = 7.47LLIMSRR7 pKa = 11.84NKK9 pKa = 10.47YY10 pKa = 10.16FGPFDD15 pKa = 5.28DD16 pKa = 4.6NDD18 pKa = 3.65YY19 pKa = 11.54NNGYY23 pKa = 10.56DD24 pKa = 5.84KK25 pKa = 11.61YY26 pKa = 11.14DD27 pKa = 3.71DD28 pKa = 4.52CNNGRR33 pKa = 11.84DD34 pKa = 4.37DD35 pKa = 5.2YY36 pKa = 11.7NSCDD40 pKa = 3.88CHH42 pKa = 6.56HH43 pKa = 7.39CCPPSCVGPTGPMGPRR59 pKa = 11.84GRR61 pKa = 11.84TGPTGPTGPTGPGVGATGPTGPTGPTGPTGNTGNTGATGLRR102 pKa = 11.84GPTGATGATGPTGATGAIGFGVTGPTGPTGATGATGADD140 pKa = 3.77GVTGPTGPTGATGADD155 pKa = 4.64GITGPTGATGATGFGVTGPTGPTGATGVGVTGATGLIGPTGATGTPGATGPTGAIGATGIGITGPTGATGATGADD230 pKa = 3.64GATGVTGPTGPTGATGADD248 pKa = 3.74GVTGPTGATGATGIGITGPTGPTGATGIGITGATGLIGPTGATGTPGATGPTGATGPTGVGVTGATGATGATGADD323 pKa = 3.64GATGVTGPTGATGATGANGLVGPTGATGAAGTPGATGPTGATGPTGVGITGATGATGATGPTGADD388 pKa = 3.47GATGPTGATGNTGADD403 pKa = 3.44GVAGPTGATGNTGADD418 pKa = 3.77GATGPTGATGATGADD433 pKa = 4.09GATGPTGATGATGVAGATGATGPTGATGADD463 pKa = 4.05GATGPTGATGATGADD478 pKa = 4.09GATGPTGATGATGVTGATGPTGPTGATGATGATGASAIIPFASGIPLSLTTIAGGLVGTPGFVGFGSSAPGLSIVGGVIDD558 pKa = 4.44LTNAAGTLTNFAFSMPRR575 pKa = 11.84DD576 pKa = 3.72GTITSISAYY585 pKa = 10.2FSTTAALSLVGSTITITATLYY606 pKa = 10.88QSTAPNNSFTAVPGATVTLAPPLTGILSVGSISSGIVTGLNIAATAQTPDD656 pKa = 2.99RR657 pKa = 11.84QYY659 pKa = 11.69AII661 pKa = 4.27

Molecular weight: 58.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q181T5|ENO_CLOD6 Enolase OS=Clostridioides difficile (strain 630) OX=272563 GN=eno PE=3 SV=1
MM1 pKa = 7.74SKK3 pKa = 8.96RR4 pKa = 11.84TYY6 pKa = 9.62QPKK9 pKa = 9.59KK10 pKa = 7.6RR11 pKa = 11.84QRR13 pKa = 11.84SKK15 pKa = 9.69EE16 pKa = 3.5HH17 pKa = 6.2GFRR20 pKa = 11.84KK21 pKa = 9.77RR22 pKa = 11.84MKK24 pKa = 9.11TSNGRR29 pKa = 11.84NVLKK33 pKa = 10.51RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 9.9GRR40 pKa = 11.84NRR42 pKa = 11.84LTHH45 pKa = 6.18

Molecular weight: 5.57 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3761

1

3762

1161445

21

2993

308.7

34.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.359 ± 0.045

1.247 ± 0.017

5.699 ± 0.031

7.398 ± 0.048

4.346 ± 0.03

6.324 ± 0.044

1.357 ± 0.014

9.916 ± 0.049

9.106 ± 0.043

9.095 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.715 ± 0.019

6.301 ± 0.04

2.741 ± 0.022

2.388 ± 0.021

3.221 ± 0.029

6.539 ± 0.033

4.926 ± 0.029

6.617 ± 0.03

0.62 ± 0.012

4.085 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski