Clostridium sp. D5

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; unclassified Clostridium

Average proteome isoelectric point is 6.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4496 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F0YVL7|F0YVL7_9CLOT Transposase IS66 (Fragment) OS=Clostridium sp. D5 OX=556261 GN=HMPREF0240_01188 PE=4 SV=1
MM1 pKa = 7.15IAGMAAGCGSGSEE14 pKa = 4.4SKK16 pKa = 10.82KK17 pKa = 10.5SDD19 pKa = 3.06SSEE22 pKa = 4.04DD23 pKa = 3.34GKK25 pKa = 11.26EE26 pKa = 4.63VVLTWQSYY34 pKa = 10.57DD35 pKa = 3.92SYY37 pKa = 11.87DD38 pKa = 3.46KK39 pKa = 11.37YY40 pKa = 11.39EE41 pKa = 4.1KK42 pKa = 10.98VVDD45 pKa = 3.86AFEE48 pKa = 5.18KK49 pKa = 10.45EE50 pKa = 4.02NPDD53 pKa = 2.86IKK55 pKa = 10.67IEE57 pKa = 4.05FEE59 pKa = 4.15EE60 pKa = 4.71VSDD63 pKa = 4.23FSTKK67 pKa = 10.05ILTEE71 pKa = 3.92ATAGDD76 pKa = 4.44LPDD79 pKa = 6.27LINCNTGTTQLLAEE93 pKa = 4.71SGALQKK99 pKa = 10.66FDD101 pKa = 3.97TEE103 pKa = 3.95EE104 pKa = 4.8LEE106 pKa = 4.51ADD108 pKa = 3.69EE109 pKa = 5.36EE110 pKa = 5.0YY111 pKa = 11.13KK112 pKa = 10.66FDD114 pKa = 5.4DD115 pKa = 3.79FWDD118 pKa = 3.9AAKK121 pKa = 9.76TYY123 pKa = 8.48CTYY126 pKa = 11.29DD127 pKa = 3.81DD128 pKa = 3.47EE129 pKa = 5.19WYY131 pKa = 10.58ALPLDD136 pKa = 4.04GGNYY140 pKa = 7.97GWVYY144 pKa = 10.07NVDD147 pKa = 3.48MFRR150 pKa = 11.84EE151 pKa = 4.29CGIEE155 pKa = 3.99VPEE158 pKa = 5.29EE159 pKa = 3.96GFTWKK164 pKa = 10.25EE165 pKa = 3.65FEE167 pKa = 4.41EE168 pKa = 4.71ACSLLNEE175 pKa = 4.59NKK177 pKa = 10.17DD178 pKa = 3.54KK179 pKa = 11.53LGIEE183 pKa = 4.42YY184 pKa = 7.99PTIFNDD190 pKa = 3.66LSSSIDD196 pKa = 3.23MMYY199 pKa = 10.86PLITEE204 pKa = 4.56AGGEE208 pKa = 4.09YY209 pKa = 10.69LNADD213 pKa = 4.14GTCAWNSEE221 pKa = 4.18AVVTAFEE228 pKa = 4.57WVQSLVAEE236 pKa = 5.22GYY238 pKa = 9.72IPSIEE243 pKa = 4.13KK244 pKa = 10.83LGDD247 pKa = 3.73GYY249 pKa = 11.38DD250 pKa = 3.53ALITKK255 pKa = 9.11FNAGQIAMCRR265 pKa = 11.84VALWNSTYY273 pKa = 11.16LEE275 pKa = 4.3DD276 pKa = 3.74TVNWKK281 pKa = 9.59VMNAPRR287 pKa = 11.84GNDD290 pKa = 4.19GIQSEE295 pKa = 4.84VLFLNGIGISNTCEE309 pKa = 3.84NYY311 pKa = 10.12DD312 pKa = 3.49AALKK316 pKa = 9.23FVKK319 pKa = 10.46YY320 pKa = 8.73LTSEE324 pKa = 4.14EE325 pKa = 4.07GLAMYY330 pKa = 10.54LKK332 pKa = 10.8DD333 pKa = 3.49NTSPQIAVRR342 pKa = 11.84RR343 pKa = 11.84SQADD347 pKa = 3.25LSVAMFDD354 pKa = 4.5EE355 pKa = 4.3KK356 pKa = 11.18HH357 pKa = 7.22DD358 pKa = 3.75MQLYY362 pKa = 7.72NTGLEE367 pKa = 4.06YY368 pKa = 10.68AGYY371 pKa = 9.99IDD373 pKa = 4.84LTNTFADD380 pKa = 3.4QQTIIGQSFDD390 pKa = 4.75EE391 pKa = 4.5IWHH394 pKa = 6.46NDD396 pKa = 2.67ADD398 pKa = 3.9IQKK401 pKa = 8.15TLDD404 pKa = 3.64SLVEE408 pKa = 3.98QLDD411 pKa = 3.86GLLTQQ416 pKa = 4.71

Molecular weight: 46.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F0YT62|F0YT62_9CLOT Alpha-L-fucosidase OS=Clostridium sp. D5 OX=556261 GN=HMPREF0240_00319 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 8.71MTFQPKK8 pKa = 7.43TRR10 pKa = 11.84QRR12 pKa = 11.84AKK14 pKa = 9.26VHH16 pKa = 5.59GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTPGGRR28 pKa = 11.84KK29 pKa = 8.8VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.18GRR39 pKa = 11.84KK40 pKa = 8.18QLSAA44 pKa = 3.9

Molecular weight: 5.0 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4496

0

4496

1527431

29

5529

339.7

38.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.604 ± 0.037

1.549 ± 0.015

5.474 ± 0.029

7.683 ± 0.043

4.098 ± 0.028

7.452 ± 0.033

1.728 ± 0.017

7.361 ± 0.035

6.453 ± 0.026

9.033 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.277 ± 0.021

4.118 ± 0.022

3.436 ± 0.021

3.279 ± 0.021

4.416 ± 0.03

5.757 ± 0.03

5.293 ± 0.029

6.881 ± 0.034

0.998 ± 0.013

4.11 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski