Erythrobacter sp. SG61-1L

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Erythrobacteraceae; Erythrobacter/Porphyrobacter group; Erythrobacter; unclassified Erythrobacter

Average proteome isoelectric point is 6.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3133 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0N8GJM0|A0A0N8GJM0_9SPHN Conjugal transfer protein TraF OS=Erythrobacter sp. SG61-1L OX=1603897 GN=SZ64_03655 PE=4 SV=1
MM1 pKa = 7.81FDD3 pKa = 4.48RR4 pKa = 11.84IVGAALVTSALLAFPAQVSAQGLSTQGDD32 pKa = 3.81SADD35 pKa = 3.56YY36 pKa = 11.01AKK38 pKa = 11.12ALTCEE43 pKa = 3.9AFYY46 pKa = 11.65VLLSQDD52 pKa = 5.07AEE54 pKa = 4.34GQDD57 pKa = 4.04DD58 pKa = 4.34GNAEE62 pKa = 3.58IDD64 pKa = 3.68AYY66 pKa = 10.89LAQTWHH72 pKa = 7.53DD73 pKa = 4.64YY74 pKa = 10.44IASTYY79 pKa = 10.03PDD81 pKa = 3.94GFNEE85 pKa = 3.95RR86 pKa = 11.84HH87 pKa = 6.17DD88 pKa = 4.1AEE90 pKa = 4.27FDD92 pKa = 3.58KK93 pKa = 11.56VADD96 pKa = 4.55DD97 pKa = 5.17LASTLNAMDD106 pKa = 4.96DD107 pKa = 3.75SSFNDD112 pKa = 3.33ALEE115 pKa = 4.53QILTTCEE122 pKa = 3.88KK123 pKa = 10.77SEE125 pKa = 4.41SDD127 pKa = 3.51PAFPGG132 pKa = 3.36

Molecular weight: 14.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0N8GK63|A0A0N8GK63_9SPHN SnoaL-like domain-containing protein OS=Erythrobacter sp. SG61-1L OX=1603897 GN=SZ64_12370 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84SRR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.53GFRR19 pKa = 11.84ARR21 pKa = 11.84TATVGGRR28 pKa = 11.84KK29 pKa = 9.07VLAARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.02KK41 pKa = 10.61LSAA44 pKa = 4.03

Molecular weight: 5.01 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3133

0

3133

991649

41

4870

316.5

34.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.781 ± 0.071

0.826 ± 0.015

5.899 ± 0.034

6.086 ± 0.044

3.64 ± 0.029

9.056 ± 0.046

1.926 ± 0.024

4.933 ± 0.026

3.316 ± 0.037

9.816 ± 0.059

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.496 ± 0.027

2.716 ± 0.037

5.222 ± 0.039

3.174 ± 0.026

6.739 ± 0.05

5.445 ± 0.042

5.094 ± 0.043

7.034 ± 0.036

1.456 ± 0.022

2.346 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski