Cohaesibacter sp. ES.047

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Cohaesibacteraceae; Cohaesibacter; unclassified Cohaesibacter

Average proteome isoelectric point is 6.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4413 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A285M074|A0A285M074_9RHIZ Histidine kinase OS=Cohaesibacter sp. ES.047 OX=1798205 GN=SAMN04515647_0760 PE=4 SV=1
MM1 pKa = 7.06ATTYY5 pKa = 11.19AVTEE9 pKa = 4.28SADD12 pKa = 3.75DD13 pKa = 3.92GTGNTSGTLSWAILQATSNGDD34 pKa = 3.58VIDD37 pKa = 3.91IDD39 pKa = 4.32ASVSSITITGSLPSASANISITTNHH64 pKa = 5.89AVEE67 pKa = 4.84VIGSALSGSGSTSLTLNGVSSAAALEE93 pKa = 4.28ATLSGTIQGHH103 pKa = 5.63NATSSGGSGSTALSGAGFILDD124 pKa = 3.54SSSNITGGKK133 pKa = 9.65ARR135 pKa = 11.84DD136 pKa = 3.69GASRR140 pKa = 11.84SYY142 pKa = 11.04SYY144 pKa = 11.58YY145 pKa = 10.66SGSNGQNGGAGGNGINAQAFSLEE168 pKa = 3.95SSGYY172 pKa = 9.38ISGGNGGNGGKK183 pKa = 9.82GASASYY189 pKa = 10.13SGRR192 pKa = 11.84AGGAGGAGGSAVSGYY207 pKa = 10.51SFSLEE212 pKa = 4.0TSGSVTGGSGGNGGNGGYY230 pKa = 10.24SLDD233 pKa = 3.57SSGGHH238 pKa = 6.0GGKK241 pKa = 10.2GAVGGASVSGTSFSLVTSGSVTGGSGGNGGAGGRR275 pKa = 11.84GGGRR279 pKa = 11.84NGGTGGIGGAGGTAISGTSFSLTNSGTLTGGDD311 pKa = 3.89AGDD314 pKa = 4.3GGNGGTSGSYY324 pKa = 10.61SSGLDD329 pKa = 3.26GGDD332 pKa = 3.16GSVGGTGGAGVSGSAFSLVNSGSITGGNGGAGGDD366 pKa = 3.85GGHH369 pKa = 6.52ATLNTTYY376 pKa = 10.66QIGGNGANGGSGGAGVSGDD395 pKa = 4.92AITLTNSGTITGGNGGAGGNAGGSGSGNAASDD427 pKa = 3.85GTDD430 pKa = 3.09GAGGVGVTGYY440 pKa = 10.71DD441 pKa = 2.95ISLVNSGTISGGLSAYY457 pKa = 10.49GLTRR461 pKa = 11.84ANAIEE466 pKa = 4.38FTSGTNSLKK475 pKa = 10.67LQSGYY480 pKa = 10.74VINGNVVANGSSDD493 pKa = 3.45TLILGGSDD501 pKa = 3.54DD502 pKa = 5.25ASFDD506 pKa = 3.99LDD508 pKa = 5.48DD509 pKa = 5.45IGSQYY514 pKa = 11.58AGFEE518 pKa = 4.54AYY520 pKa = 10.37SKK522 pKa = 9.46TGSSTWTLTGTAGEE536 pKa = 4.05AMGIFSISGGALAVNTVFDD555 pKa = 3.85SAISVLDD562 pKa = 3.66GGTLGGSGTVSNVTLYY578 pKa = 10.62SGSTIAPGNSIDD590 pKa = 3.86TLTVDD595 pKa = 3.12SDD597 pKa = 4.08LAFVSGSTYY606 pKa = 9.57EE607 pKa = 4.13VEE609 pKa = 5.12VDD611 pKa = 4.9DD612 pKa = 5.53EE613 pKa = 4.78GNSDD617 pKa = 3.8KK618 pKa = 11.02LVSTGTVSIEE628 pKa = 3.82EE629 pKa = 4.43GTTLSIIAEE638 pKa = 4.32NGTDD642 pKa = 3.79DD643 pKa = 3.9GSTYY647 pKa = 11.22AVNTDD652 pKa = 3.29YY653 pKa = 11.36SILSASSLSGAFSTIEE669 pKa = 3.66EE670 pKa = 4.2DD671 pKa = 4.52FAYY674 pKa = 10.99LDD676 pKa = 3.46ATVSYY681 pKa = 10.87NSDD684 pKa = 3.39EE685 pKa = 4.38AVLTLTRR692 pKa = 11.84ALGSSFADD700 pKa = 4.04FADD703 pKa = 3.52TTNQKK708 pKa = 9.98NVANVIEE715 pKa = 4.59GFGASDD721 pKa = 3.51SLYY724 pKa = 10.83DD725 pKa = 4.71AIVVLPDD732 pKa = 3.93GEE734 pKa = 4.34PVDD737 pKa = 5.35AFNQLTGEE745 pKa = 4.22QYY747 pKa = 10.08ATLQTIMTLNSAIDD761 pKa = 3.43RR762 pKa = 11.84NAANQRR768 pKa = 11.84LRR770 pKa = 11.84SSMGGIGGSGDD781 pKa = 3.48QVSVGFHH788 pKa = 6.06NEE790 pKa = 3.66EE791 pKa = 4.17EE792 pKa = 4.77LPSSLYY798 pKa = 7.63PTPQIWAQAFGNWSKK813 pKa = 10.78IGSTVNTASVTSQSKK828 pKa = 10.16GLLVGMDD835 pKa = 3.23AEE837 pKa = 4.58MWSDD841 pKa = 2.6WRR843 pKa = 11.84TGAFAGYY850 pKa = 9.39SQTSSKK856 pKa = 10.95ADD858 pKa = 3.95SINSAADD865 pKa = 3.0IDD867 pKa = 4.72HH868 pKa = 6.55IHH870 pKa = 6.8AGFYY874 pKa = 10.61AGTQWDD880 pKa = 3.87AFDD883 pKa = 5.59RR884 pKa = 11.84MVDD887 pKa = 3.7LNLGVSGVWHH897 pKa = 7.02HH898 pKa = 6.97IDD900 pKa = 3.68SEE902 pKa = 4.75RR903 pKa = 11.84DD904 pKa = 3.32VAFTGFSDD912 pKa = 5.21HH913 pKa = 6.64LTADD917 pKa = 3.59YY918 pKa = 11.15NAATTQAFAEE928 pKa = 3.91IGYY931 pKa = 8.41TYY933 pKa = 10.69EE934 pKa = 4.17LYY936 pKa = 10.31EE937 pKa = 4.73ARR939 pKa = 11.84LQPFLGAAIISQWSDD954 pKa = 2.96DD955 pKa = 3.59FTEE958 pKa = 4.23QGGAAALSAASATTVLGMTTLGARR982 pKa = 11.84GDD984 pKa = 3.73VQLGQFGGISASLTGSAAWQHH1005 pKa = 5.27VLGDD1009 pKa = 4.02VNSTTTMRR1017 pKa = 11.84FASGNDD1023 pKa = 3.01SFSIYY1028 pKa = 8.6GTPLDD1033 pKa = 4.0RR1034 pKa = 11.84DD1035 pKa = 3.58AAFLEE1040 pKa = 4.68AGVSFQQDD1048 pKa = 2.63EE1049 pKa = 4.41DD1050 pKa = 4.37FSLNVSYY1057 pKa = 10.86RR1058 pKa = 11.84GNFSEE1063 pKa = 4.47NANEE1067 pKa = 4.14QGLHH1071 pKa = 6.49AGFKK1075 pKa = 9.74YY1076 pKa = 10.43QFF1078 pKa = 3.62

Molecular weight: 105.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A285M2E1|A0A285M2E1_9RHIZ Multidrug resistance efflux pump OS=Cohaesibacter sp. ES.047 OX=1798205 GN=SAMN04515647_1553 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.4RR3 pKa = 11.84TYY5 pKa = 10.2QPSRR9 pKa = 11.84LVRR12 pKa = 11.84KK13 pKa = 9.21RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.48GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.31NGRR28 pKa = 11.84QILARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.95GRR39 pKa = 11.84KK40 pKa = 8.85RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4413

0

4413

1404519

39

2833

318.3

34.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.5 ± 0.043

0.952 ± 0.012

6.069 ± 0.031

6.164 ± 0.034

3.911 ± 0.026

7.884 ± 0.03

2.114 ± 0.019

5.96 ± 0.029

4.373 ± 0.03

10.085 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.816 ± 0.018

3.137 ± 0.022

4.59 ± 0.026

3.383 ± 0.021

5.888 ± 0.03

6.267 ± 0.028

5.307 ± 0.026

6.974 ± 0.029

1.26 ± 0.015

2.366 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski