Vibrio phage vB_VspP_pVa5

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Schitoviridae; Pontosvirinae; Galateavirus; Vibrio virus PVA5

Average proteome isoelectric point is 5.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 106 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1J0GV79|A0A1J0GV79_9CAUD Uncharacterized protein OS=Vibrio phage vB_VspP_pVa5 OX=1913109 GN=vBVspPpVa5_0044 PE=4 SV=1
MM1 pKa = 7.27AQHH4 pKa = 7.21TITVEE9 pKa = 3.59ADD11 pKa = 2.71INLYY15 pKa = 8.75TLSDD19 pKa = 3.76ANGDD23 pKa = 4.17CIANEE28 pKa = 4.17DD29 pKa = 3.91NVTFEE34 pKa = 4.52IDD36 pKa = 3.09SDD38 pKa = 3.89NDD40 pKa = 3.94VIIHH44 pKa = 6.31CLDD47 pKa = 3.39HH48 pKa = 6.96YY49 pKa = 11.11FIDD52 pKa = 3.91KK53 pKa = 10.88ADD55 pKa = 3.48WEE57 pKa = 4.5LLEE60 pKa = 4.45KK61 pKa = 10.82ARR63 pKa = 11.84DD64 pKa = 3.69LTQDD68 pKa = 3.14DD69 pKa = 4.22WDD71 pKa = 4.87CIALNEE77 pKa = 4.22LLEE80 pKa = 4.75DD81 pKa = 3.81KK82 pKa = 11.08

Molecular weight: 9.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1J0GVA2|A0A1J0GVA2_9CAUD Uncharacterized protein OS=Vibrio phage vB_VspP_pVa5 OX=1913109 GN=vBVspPpVa5_0087 PE=4 SV=1
MM1 pKa = 7.53SISPKK6 pKa = 9.78VIRR9 pKa = 11.84MTNSANQEE17 pKa = 3.7VRR19 pKa = 11.84VQALRR24 pKa = 11.84NGGITVSVYY33 pKa = 10.87GVGGDD38 pKa = 3.61TAQSGNQKK46 pKa = 9.85QLTVVSRR53 pKa = 11.84KK54 pKa = 9.59FGLTKK59 pKa = 10.48KK60 pKa = 10.44SVLEE64 pKa = 4.07ALQDD68 pKa = 3.45

Molecular weight: 7.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

106

0

106

24481

42

3344

231.0

25.84

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.239 ± 0.542

1.078 ± 0.172

7.136 ± 0.129

7.406 ± 0.29

3.513 ± 0.143

6.675 ± 0.274

2.124 ± 0.163

5.588 ± 0.146

6.237 ± 0.262

8.027 ± 0.232

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.064 ± 0.178

5.392 ± 0.185

3.693 ± 0.171

4.011 ± 0.227

4.175 ± 0.149

5.715 ± 0.154

6.107 ± 0.252

6.887 ± 0.21

1.328 ± 0.116

3.607 ± 0.228
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski