Mycolicibacterium tokaiense

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales;

Average proteome isoelectric point is 5.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6211 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A378TKH4|A0A378TKH4_9MYCO Peptidase S8 and S53 subtilisin kexin sedolisin OS=Mycolicibacterium tokaiense OX=39695 GN=NCTC10821_03852 PE=4 SV=1
MM1 pKa = 7.53ALIKK5 pKa = 10.64SFSVTTALFAAAFSLAAPATADD27 pKa = 3.34DD28 pKa = 4.78QMFADD33 pKa = 4.63ALDD36 pKa = 4.12MIGVSVGDD44 pKa = 3.61PAAVGRR50 pKa = 11.84GVCASFDD57 pKa = 3.52AGQTLPAVVDD67 pKa = 3.79QLSAAHH73 pKa = 7.38GITVDD78 pKa = 3.89DD79 pKa = 4.35ASMVAGFSVAEE90 pKa = 4.16YY91 pKa = 10.11CDD93 pKa = 3.51HH94 pKa = 7.5HH95 pKa = 6.97EE96 pKa = 4.51GALTLGG102 pKa = 3.8

Molecular weight: 10.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A378THS6|A0A378THS6_9MYCO Fructose-2 6-bisphosphatase OS=Mycolicibacterium tokaiense OX=39695 GN=NCTC10821_02638 PE=4 SV=1
MM1 pKa = 7.69AKK3 pKa = 10.06GKK5 pKa = 8.69RR6 pKa = 11.84TFQPNNRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84ARR17 pKa = 11.84VHH19 pKa = 5.99GFRR22 pKa = 11.84LRR24 pKa = 11.84MRR26 pKa = 11.84TRR28 pKa = 11.84AGRR31 pKa = 11.84AIVTGRR37 pKa = 11.84RR38 pKa = 11.84RR39 pKa = 11.84KK40 pKa = 9.68GRR42 pKa = 11.84RR43 pKa = 11.84SLTAA47 pKa = 3.9

Molecular weight: 5.56 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6211

0

6211

1976286

29

5196

318.2

34.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.312 ± 0.042

0.786 ± 0.009

6.276 ± 0.028

5.095 ± 0.029

3.066 ± 0.023

8.926 ± 0.034

2.184 ± 0.016

4.181 ± 0.021

1.894 ± 0.021

10.047 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.026 ± 0.011

2.118 ± 0.016

5.747 ± 0.03

3.066 ± 0.017

7.068 ± 0.032

5.41 ± 0.022

6.286 ± 0.025

8.959 ± 0.031

1.481 ± 0.014

2.072 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski