Rathayibacter toxicus

Taxonomy: cellular organisms; Bacteria;

Average proteome isoelectric point is 6.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1904 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0U1PW50|A0A0U1PW50_9MICO Uncharacterized protein OS=Rathayibacter toxicus OX=145458 GN=VT73_00500 PE=4 SV=1
MM1 pKa = 7.84LLRR4 pKa = 11.84EE5 pKa = 4.15YY6 pKa = 10.95VGVIRR11 pKa = 11.84AQDD14 pKa = 3.18IDD16 pKa = 3.87LTDD19 pKa = 3.15YY20 pKa = 10.74FYY22 pKa = 11.01QLQGAWLDD30 pKa = 4.19FQDD33 pKa = 3.51QCPWAVIEE41 pKa = 4.09EE42 pKa = 4.23ATFIGGFSVFVHH54 pKa = 6.06EE55 pKa = 5.64NGAEE59 pKa = 4.18TDD61 pKa = 3.0WLEE64 pKa = 4.16RR65 pKa = 11.84VAEE68 pKa = 4.17AFEE71 pKa = 4.16LAGGGALSTVALQAVSGG88 pKa = 3.91

Molecular weight: 9.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0C5BSY4|A0A0C5BSY4_9MICO Holliday junction ATP-dependent DNA helicase RuvA OS=Rathayibacter toxicus OX=145458 GN=ruvA PE=3 SV=1
MM1 pKa = 6.39TTEE4 pKa = 4.33NSSSSALSVRR14 pKa = 11.84ARR16 pKa = 11.84RR17 pKa = 11.84STTQRR22 pKa = 11.84RR23 pKa = 11.84RR24 pKa = 11.84AEE26 pKa = 3.81SRR28 pKa = 11.84FAFWLVLPTVILLSAVIASPIIQAIWRR55 pKa = 11.84SLFSDD60 pKa = 4.33TITGKK65 pKa = 10.25ISFIGIQNYY74 pKa = 10.12LSIFIGSSSSQFWDD88 pKa = 3.13AMGVTVFLTIVSVTLEE104 pKa = 4.23TVLGVSMALVMNRR117 pKa = 11.84AFRR120 pKa = 11.84GRR122 pKa = 11.84GIIRR126 pKa = 11.84AAILVPWAIPTAVCAVLWRR145 pKa = 11.84WTFDD149 pKa = 3.05ANGIVNSLLGHH160 pKa = 6.1QILWTAQEE168 pKa = 4.27WPAKK172 pKa = 9.5IAIIFADD179 pKa = 3.56TWKK182 pKa = 8.78TAPFIALLVLAGLQMIPTEE201 pKa = 4.31IIEE204 pKa = 4.11ASKK207 pKa = 10.43MDD209 pKa = 3.64GAGAWRR215 pKa = 11.84RR216 pKa = 11.84FWTITFPLVRR226 pKa = 11.84PALTVAVLFRR236 pKa = 11.84LLDD239 pKa = 3.28VLRR242 pKa = 11.84IYY244 pKa = 10.55DD245 pKa = 4.27LPQIFTGGANNTTTLSMLVVRR266 pKa = 11.84EE267 pKa = 4.33SIGNLHH273 pKa = 6.87AGLGSALSTVTFTFIFLCAFAFIRR297 pKa = 11.84MLGAQVFPDD306 pKa = 3.69EE307 pKa = 4.55DD308 pKa = 3.31QHH310 pKa = 8.0RR311 pKa = 3.93

Molecular weight: 34.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1904

0

1904

586753

35

2329

308.2

33.14

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.345 ± 0.08

0.655 ± 0.014

5.69 ± 0.043

5.677 ± 0.056

3.129 ± 0.029

8.534 ± 0.053

2.125 ± 0.026

4.788 ± 0.045

2.206 ± 0.041

10.377 ± 0.067

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.665 ± 0.02

2.239 ± 0.035

5.113 ± 0.04

2.915 ± 0.032

7.483 ± 0.063

6.455 ± 0.039

6.211 ± 0.048

9.02 ± 0.06

1.336 ± 0.023

2.038 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski