Amino acid dipepetide frequency for Metallosphaera yellowstonensis MK1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.058AlaAla: 3.058 ± 0.091
0.518AlaCys: 0.518 ± 0.024
2.304AlaAsp: 2.304 ± 0.051
4.003AlaGlu: 4.003 ± 0.077
2.842AlaPhe: 2.842 ± 0.07
4.118AlaGly: 4.118 ± 0.076
0.864AlaHis: 0.864 ± 0.031
4.213AlaIle: 4.213 ± 0.071
3.74AlaLys: 3.74 ± 0.081
7.835AlaLeu: 7.835 ± 0.099
1.663AlaMet: 1.663 ± 0.046
1.795AlaAsn: 1.795 ± 0.052
1.935AlaPro: 1.935 ± 0.054
1.422AlaGln: 1.422 ± 0.051
3.588AlaArg: 3.588 ± 0.086
4.306AlaSer: 4.306 ± 0.074
2.896AlaThr: 2.896 ± 0.069
5.574AlaVal: 5.574 ± 0.107
0.771AlaTrp: 0.771 ± 0.034
2.392AlaTyr: 2.392 ± 0.057
0.0AlaXaa: 0.0 ± 0.0
Cys
0.258CysAla: 0.258 ± 0.019
0.089CysCys: 0.089 ± 0.012
0.311CysAsp: 0.311 ± 0.02
0.459CysGlu: 0.459 ± 0.03
0.18CysPhe: 0.18 ± 0.017
0.775CysGly: 0.775 ± 0.036
0.2CysHis: 0.2 ± 0.015
0.308CysIle: 0.308 ± 0.023
0.354CysLys: 0.354 ± 0.021
0.551CysLeu: 0.551 ± 0.025
0.154CysMet: 0.154 ± 0.015
0.268CysAsn: 0.268 ± 0.02
0.599CysPro: 0.599 ± 0.031
0.198CysGln: 0.198 ± 0.016
0.39CysArg: 0.39 ± 0.024
0.498CysSer: 0.498 ± 0.027
0.295CysThr: 0.295 ± 0.023
0.451CysVal: 0.451 ± 0.025
0.069CysTrp: 0.069 ± 0.01
0.258CysTyr: 0.258 ± 0.017
0.0CysXaa: 0.0 ± 0.0
Asp
2.561AspAla: 2.561 ± 0.059
0.32AspCys: 0.32 ± 0.02
1.908AspAsp: 1.908 ± 0.057
3.355AspGlu: 3.355 ± 0.077
2.048AspPhe: 2.048 ± 0.052
3.113AspGly: 3.113 ± 0.077
0.774AspHis: 0.774 ± 0.031
2.611AspIle: 2.611 ± 0.059
2.574AspLys: 2.574 ± 0.067
5.443AspLeu: 5.443 ± 0.088
1.142AspMet: 1.142 ± 0.035
1.33AspAsn: 1.33 ± 0.042
2.655AspPro: 2.655 ± 0.063
1.011AspGln: 1.011 ± 0.033
2.546AspArg: 2.546 ± 0.061
2.81AspSer: 2.81 ± 0.065
1.718AspThr: 1.718 ± 0.047
5.006AspVal: 5.006 ± 0.082
0.617AspTrp: 0.617 ± 0.028
2.036AspTyr: 2.036 ± 0.048
0.0AspXaa: 0.0 ± 0.0
Glu
4.757GluAla: 4.757 ± 0.088
0.394GluCys: 0.394 ± 0.023
3.684GluAsp: 3.684 ± 0.074
6.92GluGlu: 6.92 ± 0.125
2.528GluPhe: 2.528 ± 0.063
5.251GluGly: 5.251 ± 0.094
0.868GluHis: 0.868 ± 0.038
5.269GluIle: 5.269 ± 0.087
4.518GluLys: 4.518 ± 0.107
7.782GluLeu: 7.782 ± 0.109
1.94GluMet: 1.94 ± 0.056
2.294GluAsn: 2.294 ± 0.055
1.994GluPro: 1.994 ± 0.047
1.415GluGln: 1.415 ± 0.055
4.978GluArg: 4.978 ± 0.097
3.504GluSer: 3.504 ± 0.067
2.974GluThr: 2.974 ± 0.059
7.305GluVal: 7.305 ± 0.113
0.719GluTrp: 0.719 ± 0.034
2.407GluTyr: 2.407 ± 0.049
0.0GluXaa: 0.0 ± 0.0
Phe
2.27PheAla: 2.27 ± 0.061
0.246PheCys: 0.246 ± 0.019
1.727PheAsp: 1.727 ± 0.05
1.88PheGlu: 1.88 ± 0.053
1.77PhePhe: 1.77 ± 0.057
2.71PheGly: 2.71 ± 0.063
0.724PheHis: 0.724 ± 0.03
2.423PheIle: 2.423 ± 0.064
2.571PheLys: 2.571 ± 0.055
5.123PheLeu: 5.123 ± 0.089
1.03PheMet: 1.03 ± 0.036
1.711PheAsn: 1.711 ± 0.049
2.147PhePro: 2.147 ± 0.052
1.14PheGln: 1.14 ± 0.04
2.397PheArg: 2.397 ± 0.051
3.638PheSer: 3.638 ± 0.085
2.452PheThr: 2.452 ± 0.058
3.283PheVal: 3.283 ± 0.07
0.526PheTrp: 0.526 ± 0.025
1.843PheTyr: 1.843 ± 0.048
0.001PheXaa: 0.001 ± 0.001
Gly
4.076GlyAla: 4.076 ± 0.093
0.46GlyCys: 0.46 ± 0.028
3.369GlyAsp: 3.369 ± 0.067
5.584GlyGlu: 5.584 ± 0.088
3.388GlyPhe: 3.388 ± 0.061
5.622GlyGly: 5.622 ± 0.101
1.217GlyHis: 1.217 ± 0.041
5.42GlyIle: 5.42 ± 0.089
5.422GlyLys: 5.422 ± 0.095
7.48GlyLeu: 7.48 ± 0.107
1.992GlyMet: 1.992 ± 0.046
2.619GlyAsn: 2.619 ± 0.066
2.155GlyPro: 2.155 ± 0.061
1.591GlyGln: 1.591 ± 0.056
4.832GlyArg: 4.832 ± 0.09
4.685GlySer: 4.685 ± 0.081
3.575GlyThr: 3.575 ± 0.081
6.887GlyVal: 6.887 ± 0.105
1.019GlyTrp: 1.019 ± 0.034
3.137GlyTyr: 3.137 ± 0.078
0.0GlyXaa: 0.0 ± 0.0
His
0.775HisAla: 0.775 ± 0.034
0.196HisCys: 0.196 ± 0.015
0.67HisAsp: 0.67 ± 0.029
0.986HisGlu: 0.986 ± 0.043
0.636HisPhe: 0.636 ± 0.032
1.366HisGly: 1.366 ± 0.045
0.35HisHis: 0.35 ± 0.021
0.722HisIle: 0.722 ± 0.033
0.767HisLys: 0.767 ± 0.032
1.599HisLeu: 1.599 ± 0.046
0.38HisMet: 0.38 ± 0.024
0.446HisAsn: 0.446 ± 0.022
0.819HisPro: 0.819 ± 0.033
0.363HisGln: 0.363 ± 0.022
0.937HisArg: 0.937 ± 0.035
0.972HisSer: 0.972 ± 0.034
0.679HisThr: 0.679 ± 0.03
1.405HisVal: 1.405 ± 0.047
0.176HisTrp: 0.176 ± 0.015
0.612HisTyr: 0.612 ± 0.028
0.0HisXaa: 0.0 ± 0.0
Ile
4.423IleAla: 4.423 ± 0.086
0.351IleCys: 0.351 ± 0.021
2.947IleAsp: 2.947 ± 0.063
3.995IleGlu: 3.995 ± 0.078
2.736IlePhe: 2.736 ± 0.072
4.217IleGly: 4.217 ± 0.084
1.059IleHis: 1.059 ± 0.037
4.306IleIle: 4.306 ± 0.103
3.756IleLys: 3.756 ± 0.07
6.914IleLeu: 6.914 ± 0.112
1.58IleMet: 1.58 ± 0.052
2.501IleAsn: 2.501 ± 0.066
3.507IlePro: 3.507 ± 0.079
1.529IleGln: 1.529 ± 0.044
3.66IleArg: 3.66 ± 0.087
5.328IleSer: 5.328 ± 0.093
3.54IleThr: 3.54 ± 0.069
5.101IleVal: 5.101 ± 0.078
0.547IleTrp: 0.547 ± 0.029
2.663IleTyr: 2.663 ± 0.064
0.0IleXaa: 0.0 ± 0.0
Lys
3.901LysAla: 3.901 ± 0.086
0.45LysCys: 0.45 ± 0.025
3.117LysAsp: 3.117 ± 0.066
5.617LysGlu: 5.617 ± 0.103
2.35LysPhe: 2.35 ± 0.061
5.067LysGly: 5.067 ± 0.088
0.618LysHis: 0.618 ± 0.029
4.091LysIle: 4.091 ± 0.086
3.452LysLys: 3.452 ± 0.086
6.398LysLeu: 6.398 ± 0.106
1.608LysMet: 1.608 ± 0.049
1.77LysAsn: 1.77 ± 0.056
1.976LysPro: 1.976 ± 0.054
0.984LysGln: 0.984 ± 0.031
4.111LysArg: 4.111 ± 0.1
3.402LysSer: 3.402 ± 0.068
2.504LysThr: 2.504 ± 0.06
6.517LysVal: 6.517 ± 0.109
0.63LysTrp: 0.63 ± 0.028
2.585LysTyr: 2.585 ± 0.053
0.0LysXaa: 0.0 ± 0.0
Leu
7.169LeuAla: 7.169 ± 0.111
0.491LeuCys: 0.491 ± 0.03
5.23LeuAsp: 5.23 ± 0.103
7.458LeuGlu: 7.458 ± 0.12
4.096LeuPhe: 4.096 ± 0.084
8.63LeuGly: 8.63 ± 0.13
1.568LeuHis: 1.568 ± 0.05
6.814LeuIle: 6.814 ± 0.112
6.868LeuLys: 6.868 ± 0.108
11.569LeuLeu: 11.569 ± 0.188
2.771LeuMet: 2.771 ± 0.069
4.409LeuAsn: 4.409 ± 0.082
4.442LeuPro: 4.442 ± 0.081
2.44LeuGln: 2.44 ± 0.056
7.616LeuArg: 7.616 ± 0.125
8.392LeuSer: 8.392 ± 0.125
5.628LeuThr: 5.628 ± 0.096
9.038LeuVal: 9.038 ± 0.112
1.129LeuTrp: 1.129 ± 0.041
3.835LeuTyr: 3.835 ± 0.074
0.0LeuXaa: 0.0 ± 0.0
Met
1.746MetAla: 1.746 ± 0.052
0.137MetCys: 0.137 ± 0.013
1.219MetAsp: 1.219 ± 0.044
1.922MetGlu: 1.922 ± 0.052
0.844MetPhe: 0.844 ± 0.032
2.141MetGly: 2.141 ± 0.056
0.26MetHis: 0.26 ± 0.017
1.926MetIle: 1.926 ± 0.044
1.86MetLys: 1.86 ± 0.053
2.123MetLeu: 2.123 ± 0.056
0.682MetMet: 0.682 ± 0.031
0.982MetAsn: 0.982 ± 0.035
0.829MetPro: 0.829 ± 0.034
0.406MetGln: 0.406 ± 0.022
2.04MetArg: 2.04 ± 0.055
1.768MetSer: 1.768 ± 0.047
1.348MetThr: 1.348 ± 0.042
1.914MetVal: 1.914 ± 0.048
0.302MetTrp: 0.302 ± 0.021
0.689MetTyr: 0.689 ± 0.03
0.0MetXaa: 0.0 ± 0.0
Asn
2.182AsnAla: 2.182 ± 0.053
0.27AsnCys: 0.27 ± 0.022
1.41AsnAsp: 1.41 ± 0.043
2.202AsnGlu: 2.202 ± 0.058
1.692AsnPhe: 1.692 ± 0.052
2.474AsnGly: 2.474 ± 0.085
0.446AsnHis: 0.446 ± 0.025
1.988AsnIle: 1.988 ± 0.058
1.807AsnLys: 1.807 ± 0.058
4.235AsnLeu: 4.235 ± 0.075
0.87AsnMet: 0.87 ± 0.037
1.356AsnAsn: 1.356 ± 0.06
2.107AsnPro: 2.107 ± 0.059
0.876AsnGln: 0.876 ± 0.033
1.812AsnArg: 1.812 ± 0.054
2.589AsnSer: 2.589 ± 0.069
1.556AsnThr: 1.556 ± 0.05
3.583AsnVal: 3.583 ± 0.089
0.435AsnTrp: 0.435 ± 0.024
1.866AsnTyr: 1.866 ± 0.057
0.0AsnXaa: 0.0 ± 0.0
Pro
1.953ProAla: 1.953 ± 0.06
0.259ProCys: 0.259 ± 0.02
1.871ProAsp: 1.871 ± 0.05
2.96ProGlu: 2.96 ± 0.076
2.071ProPhe: 2.071 ± 0.053
2.968ProGly: 2.968 ± 0.07
0.831ProHis: 0.831 ± 0.035
2.461ProIle: 2.461 ± 0.065
2.487ProLys: 2.487 ± 0.053
4.738ProLeu: 4.738 ± 0.086
0.981ProMet: 0.981 ± 0.037
1.599ProAsn: 1.599 ± 0.052
2.285ProPro: 2.285 ± 0.061
1.27ProGln: 1.27 ± 0.044
2.15ProArg: 2.15 ± 0.057
3.459ProSer: 3.459 ± 0.062
2.189ProThr: 2.189 ± 0.057
3.717ProVal: 3.717 ± 0.076
0.666ProTrp: 0.666 ± 0.03
1.822ProTyr: 1.822 ± 0.052
0.0ProXaa: 0.0 ± 0.0
Gln
1.453GlnAla: 1.453 ± 0.046
0.127GlnCys: 0.127 ± 0.012
1.104GlnAsp: 1.104 ± 0.04
1.899GlnGlu: 1.899 ± 0.049
0.943GlnPhe: 0.943 ± 0.035
2.243GlnGly: 2.243 ± 0.068
0.263GlnHis: 0.263 ± 0.017
1.444GlnIle: 1.444 ± 0.044
1.034GlnLys: 1.034 ± 0.041
2.355GlnLeu: 2.355 ± 0.06
0.539GlnMet: 0.539 ± 0.024
0.745GlnAsn: 0.745 ± 0.033
0.8GlnPro: 0.8 ± 0.036
0.648GlnGln: 0.648 ± 0.031
1.291GlnArg: 1.291 ± 0.044
1.186GlnSer: 1.186 ± 0.041
1.059GlnThr: 1.059 ± 0.038
2.403GlnVal: 2.403 ± 0.059
0.257GlnTrp: 0.257 ± 0.02
0.86GlnTyr: 0.86 ± 0.036
0.0GlnXaa: 0.0 ± 0.0
Arg
4.034ArgAla: 4.034 ± 0.078
0.474ArgCys: 0.474 ± 0.028
3.443ArgAsp: 3.443 ± 0.068
6.315ArgGlu: 6.315 ± 0.117
2.286ArgPhe: 2.286 ± 0.055
5.242ArgGly: 5.242 ± 0.099
0.884ArgHis: 0.884 ± 0.034
3.982ArgIle: 3.982 ± 0.083
4.317ArgLys: 4.317 ± 0.079
5.797ArgLeu: 5.797 ± 0.1
1.466ArgMet: 1.466 ± 0.038
2.153ArgAsn: 2.153 ± 0.054
2.178ArgPro: 2.178 ± 0.062
1.159ArgGln: 1.159 ± 0.038
5.194ArgArg: 5.194 ± 0.098
3.482ArgSer: 3.482 ± 0.063
2.75ArgThr: 2.75 ± 0.07
5.39ArgVal: 5.39 ± 0.103
0.741ArgTrp: 0.741 ± 0.035
2.119ArgTyr: 2.119 ± 0.054
0.0ArgXaa: 0.0 ± 0.0
Ser
3.625SerAla: 3.625 ± 0.066
0.537SerCys: 0.537 ± 0.028
2.591SerAsp: 2.591 ± 0.056
3.678SerGlu: 3.678 ± 0.08
3.249SerPhe: 3.249 ± 0.066
4.843SerGly: 4.843 ± 0.088
1.168SerHis: 1.168 ± 0.043
4.196SerIle: 4.196 ± 0.085
4.137SerLys: 4.137 ± 0.085
8.952SerLeu: 8.952 ± 0.137
1.821SerMet: 1.821 ± 0.042
2.255SerAsn: 2.255 ± 0.059
3.822SerPro: 3.822 ± 0.076
1.874SerGln: 1.874 ± 0.052
4.34SerArg: 4.34 ± 0.083
5.916SerSer: 5.916 ± 0.102
3.81SerThr: 3.81 ± 0.084
5.322SerVal: 5.322 ± 0.09
0.999SerTrp: 0.999 ± 0.036
2.69SerTyr: 2.69 ± 0.057
0.0SerXaa: 0.0 ± 0.0
Thr
3.083ThrAla: 3.083 ± 0.065
0.442ThrCys: 0.442 ± 0.023
1.924ThrAsp: 1.924 ± 0.053
2.57ThrGlu: 2.57 ± 0.062
2.397ThrPhe: 2.397 ± 0.057
3.874ThrGly: 3.874 ± 0.07
0.746ThrHis: 0.746 ± 0.034
2.969ThrIle: 2.969 ± 0.07
2.493ThrLys: 2.493 ± 0.068
5.943ThrLeu: 5.943 ± 0.103
1.137ThrMet: 1.137 ± 0.039
1.712ThrAsn: 1.712 ± 0.052
2.663ThrPro: 2.663 ± 0.068
1.292ThrGln: 1.292 ± 0.042
2.384ThrArg: 2.384 ± 0.06
3.903ThrSer: 3.903 ± 0.084
2.741ThrThr: 2.741 ± 0.079
4.439ThrVal: 4.439 ± 0.093
0.601ThrTrp: 0.601 ± 0.027
1.873ThrTyr: 1.873 ± 0.063
0.0ThrXaa: 0.0 ± 0.0
Val
5.224ValAla: 5.224 ± 0.082
0.559ValCys: 0.559 ± 0.028
4.258ValAsp: 4.258 ± 0.084
6.753ValGlu: 6.753 ± 0.113
3.271ValPhe: 3.271 ± 0.075
6.143ValGly: 6.143 ± 0.1
1.222ValHis: 1.222 ± 0.04
6.64ValIle: 6.64 ± 0.083
6.463ValLys: 6.463 ± 0.102
8.965ValLeu: 8.965 ± 0.123
2.256ValMet: 2.256 ± 0.066
3.657ValAsn: 3.657 ± 0.095
3.66ValPro: 3.66 ± 0.085
1.787ValGln: 1.787 ± 0.053
5.982ValArg: 5.982 ± 0.102
6.114ValSer: 6.114 ± 0.104
4.773ValThr: 4.773 ± 0.093
8.291ValVal: 8.291 ± 0.125
0.768ValTrp: 0.768 ± 0.03
3.341ValTyr: 3.341 ± 0.077
0.0ValXaa: 0.0 ± 0.0
Trp
0.704TrpAla: 0.704 ± 0.031
0.062TrpCys: 0.062 ± 0.008
0.595TrpAsp: 0.595 ± 0.028
0.841TrpGlu: 0.841 ± 0.037
0.492TrpPhe: 0.492 ± 0.024
0.794TrpGly: 0.794 ± 0.035
0.179TrpHis: 0.179 ± 0.016
0.796TrpIle: 0.796 ± 0.035
0.67TrpLys: 0.67 ± 0.028
1.156TrpLeu: 1.156 ± 0.049
0.303TrpMet: 0.303 ± 0.021
0.543TrpAsn: 0.543 ± 0.03
0.347TrpPro: 0.347 ± 0.021
0.214TrpGln: 0.214 ± 0.017
1.125TrpArg: 1.125 ± 0.044
0.744TrpSer: 0.744 ± 0.028
0.591TrpThr: 0.591 ± 0.031
0.846TrpVal: 0.846 ± 0.035
0.166TrpTrp: 0.166 ± 0.015
0.565TrpTyr: 0.565 ± 0.029
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.588TyrAla: 2.588 ± 0.061
0.283TyrCys: 0.283 ± 0.02
1.892TyrAsp: 1.892 ± 0.053
2.106TyrGlu: 2.106 ± 0.054
1.795TyrPhe: 1.795 ± 0.05
2.94TyrGly: 2.94 ± 0.061
0.614TyrHis: 0.614 ± 0.029
2.079TyrIle: 2.079 ± 0.058
1.86TyrLys: 1.86 ± 0.053
4.637TyrLeu: 4.637 ± 0.088
0.823TyrMet: 0.823 ± 0.032
1.612TyrAsn: 1.612 ± 0.053
1.813TyrPro: 1.813 ± 0.051
1.033TyrGln: 1.033 ± 0.048
2.032TyrArg: 2.032 ± 0.055
3.128TyrSer: 3.128 ± 0.061
2.107TyrThr: 2.107 ± 0.055
3.657TyrVal: 3.657 ± 0.075
0.595TyrTrp: 0.595 ± 0.036
1.869TyrTyr: 1.869 ± 0.063
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.001XaaPro: 0.001 ± 0.001
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3340 proteins (771653 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski