Enterobacteria phage phi80 (Bacteriophage phi-80)

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 63 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M9NZA3|M9NZA3_BPPH8 Tail assembly protein OS=Enterobacteria phage phi80 OX=10713 GN=PHI80_19 PE=4 SV=1
MM1 pKa = 7.4LAGMTSSEE9 pKa = 4.35LGDD12 pKa = 2.9WHH14 pKa = 6.44QFYY17 pKa = 10.32RR18 pKa = 11.84DD19 pKa = 4.07HH20 pKa = 6.76YY21 pKa = 10.12FQDD24 pKa = 3.64AQLDD28 pKa = 3.72AHH30 pKa = 6.69FSEE33 pKa = 4.9LLYY36 pKa = 11.01SISTLFFRR44 pKa = 11.84DD45 pKa = 3.71PEE47 pKa = 4.13LTPAHH52 pKa = 6.63FSLLSPSDD60 pKa = 3.71SVISDD65 pKa = 4.68DD66 pKa = 5.51EE67 pKa = 5.64PDD69 pKa = 5.1DD70 pKa = 4.14DD71 pKa = 4.64TLMTAAEE78 pKa = 5.28GITGGIRR85 pKa = 11.84YY86 pKa = 9.1GPADD90 pKa = 3.26

Molecular weight: 10.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M9NYX5|M9NYX5_BPPH8 Uncharacterized protein OS=Enterobacteria phage phi80 OX=10713 GN=PHI80_41 PE=4 SV=1
MM1 pKa = 7.32EE2 pKa = 4.7MKK4 pKa = 9.61RR5 pKa = 11.84TPFYY9 pKa = 10.6RR10 pKa = 11.84RR11 pKa = 11.84PGRR14 pKa = 11.84AGQFSGLRR22 pKa = 11.84EE23 pKa = 3.76RR24 pKa = 11.84VIWMIQTRR32 pKa = 11.84GRR34 pKa = 11.84PVTGSEE40 pKa = 3.59IAEE43 pKa = 4.17KK44 pKa = 10.54FGVSLIEE51 pKa = 4.12FNRR54 pKa = 11.84VANGITRR61 pKa = 11.84GTGQIAQIVEE71 pKa = 3.97SEE73 pKa = 3.7KK74 pKa = 10.36WINEE78 pKa = 3.81DD79 pKa = 4.25GICDD83 pKa = 3.49RR84 pKa = 11.84TFDD87 pKa = 4.74LVTKK91 pKa = 9.34PKK93 pKa = 10.75VITPQGKK100 pKa = 8.89SRR102 pKa = 11.84LFTRR106 pKa = 11.84RR107 pKa = 11.84AIEE110 pKa = 3.69QSQEE114 pKa = 3.46GRR116 pKa = 11.84RR117 pKa = 11.84QEE119 pKa = 4.53CIEE122 pKa = 3.63RR123 pKa = 11.84AARR126 pKa = 11.84RR127 pKa = 11.84RR128 pKa = 11.84RR129 pKa = 11.84LIAQGLYY136 pKa = 8.85IDD138 pKa = 4.34EE139 pKa = 4.72MEE141 pKa = 5.46SILL144 pKa = 4.97

Molecular weight: 16.64 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

63

0

63

13775

56

1192

218.7

24.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.917 ± 0.577

1.227 ± 0.175

5.975 ± 0.272

6.417 ± 0.393

3.361 ± 0.199

7.093 ± 0.308

1.575 ± 0.182

5.437 ± 0.241

5.684 ± 0.37

7.528 ± 0.227

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.918 ± 0.205

4.312 ± 0.242

3.797 ± 0.296

4.668 ± 0.441

6.229 ± 0.423

6.817 ± 0.294

6.047 ± 0.389

6.359 ± 0.285

1.75 ± 0.137

2.889 ± 0.147
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski