Clostridium bartlettii CAG:1329

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; environmental samples

Average proteome isoelectric point is 6.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2686 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R5XNK5|R5XNK5_9CLOT Lar_N domain-containing protein OS=Clostridium bartlettii CAG:1329 OX=1263063 GN=BN488_01405 PE=4 SV=1
MM1 pKa = 7.96RR2 pKa = 11.84EE3 pKa = 3.72ATLPFTVTKK12 pKa = 10.47SVNPTSAGLNDD23 pKa = 3.65TVTYY27 pKa = 9.45TVVLNNTSANTLSNATFVDD46 pKa = 3.72QLPAGLEE53 pKa = 4.12YY54 pKa = 11.01VPNTLTVNGQAKK66 pKa = 10.46AGVDD70 pKa = 3.57PNTQFTIDD78 pKa = 5.02DD79 pKa = 3.59IPGNGNATVTFDD91 pKa = 3.72AKK93 pKa = 10.77VVSLPATPKK102 pKa = 10.1TEE104 pKa = 3.75NTAQFGFSYY113 pKa = 10.45TPITGSPAQTLNLSSTEE130 pKa = 3.94ANPLYY135 pKa = 10.52IGDD138 pKa = 4.14AVIGSTNFTKK148 pKa = 10.39TAGATSAKK156 pKa = 10.27VGDD159 pKa = 4.53EE160 pKa = 3.87IPYY163 pKa = 9.58TIAISNGGNVNATNVTLTDD182 pKa = 3.99ALPPGTTLVADD193 pKa = 4.06SLTVTNKK200 pKa = 7.36QTGANVDD207 pKa = 3.63YY208 pKa = 10.77TGDD211 pKa = 3.69LASGLNLTNALQPGTANEE229 pKa = 3.87VDD231 pKa = 3.35VSYY234 pKa = 10.98RR235 pKa = 11.84ALVNTAPVGNTLTNTANLSYY255 pKa = 10.48QYY257 pKa = 10.76QRR259 pKa = 11.84NPNDD263 pKa = 3.57PTTVTQTATSSANVNIEE280 pKa = 3.8NASDD284 pKa = 3.73YY285 pKa = 10.75PITITKK291 pKa = 7.62TANPANNLVVGDD303 pKa = 3.91VVTYY307 pKa = 9.08TVTVEE312 pKa = 3.92NTSTNNATYY321 pKa = 10.3TNTTITDD328 pKa = 3.91TLPDD332 pKa = 3.45NLTYY336 pKa = 10.73EE337 pKa = 4.44RR338 pKa = 11.84GSVTINGQTSTQEE351 pKa = 3.83LTNGITLEE359 pKa = 4.18NDD361 pKa = 3.74LAPNVPTTVTFRR373 pKa = 11.84AVVNSIPQNKK383 pKa = 8.47TISNTASINYY393 pKa = 7.8DD394 pKa = 3.06ATVGGEE400 pKa = 3.92IKK402 pKa = 10.19QQNIDD407 pKa = 3.57ATSEE411 pKa = 4.44AITSNVVNASLNVTKK426 pKa = 10.24TDD428 pKa = 3.33NAAFTLAIGDD438 pKa = 3.96TFNYY442 pKa = 8.31TISVSNTGEE451 pKa = 3.98VPLNNVTVTDD461 pKa = 4.01NLPSQLEE468 pKa = 4.25VTQISVDD475 pKa = 3.55GAPIDD480 pKa = 3.73TLLTNNIPLGDD491 pKa = 3.74MNANEE496 pKa = 4.44TKK498 pKa = 10.24TVVISVKK505 pKa = 10.3AASGGLNNFQNTAIASSQVNIEE527 pKa = 3.97GRR529 pKa = 11.84TEE531 pKa = 3.58PAEE534 pKa = 3.97ISISGSDD541 pKa = 3.46NNAITIPTNPTPIIPTPTPNLLNLASINSIASKK574 pKa = 10.18NISYY578 pKa = 10.79VGDD581 pKa = 4.55AISYY585 pKa = 7.71ATIVSNVGNVPLGTYY600 pKa = 9.36DD601 pKa = 3.39VPVTVFTEE609 pKa = 4.25LSPSVSFIPGSLSINNVPVNCVNPSQINLGILQPGDD645 pKa = 3.41VRR647 pKa = 11.84IITYY651 pKa = 7.75SVRR654 pKa = 11.84VNNACPNIVYY664 pKa = 10.38NIQKK668 pKa = 10.17LLYY671 pKa = 9.75GYY673 pKa = 10.29DD674 pKa = 3.48PLYY677 pKa = 10.59IGTSTFSVNGNIVMTYY693 pKa = 10.38INN695 pKa = 3.81

Molecular weight: 72.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R5XGS9|R5XGS9_9CLOT Ion-translocating oxidoreductase complex subunit D OS=Clostridium bartlettii CAG:1329 OX=1263063 GN=rnfD PE=3 SV=1
MM1 pKa = 7.36KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 9.67QPKK8 pKa = 9.0KK9 pKa = 7.87RR10 pKa = 11.84QRR12 pKa = 11.84KK13 pKa = 8.39KK14 pKa = 8.49EE15 pKa = 3.5HH16 pKa = 6.11GFRR19 pKa = 11.84KK20 pKa = 9.77RR21 pKa = 11.84MKK23 pKa = 9.11TSNGRR28 pKa = 11.84NVLKK32 pKa = 10.51RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.9GRR39 pKa = 11.84NRR41 pKa = 11.84LTHH44 pKa = 6.18

Molecular weight: 5.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2686

0

2686

839455

29

2011

312.5

35.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.61 ± 0.049

1.361 ± 0.022

6.12 ± 0.046

7.36 ± 0.058

4.237 ± 0.034

6.313 ± 0.051

1.34 ± 0.018

9.621 ± 0.058

9.131 ± 0.057

8.85 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.682 ± 0.023

6.319 ± 0.048

2.684 ± 0.027

2.567 ± 0.023

3.177 ± 0.03

6.112 ± 0.042

5.023 ± 0.04

6.627 ± 0.043

0.581 ± 0.012

4.285 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski