Human gemycircularvirus GeTz1

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Genomoviridae; Gemykibivirus; Gemykibivirus humas4; Human associated gemykibivirus 4

Average proteome isoelectric point is 6.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A125R3J9|A0A125R3J9_9VIRU Uncharacterized protein OS=Human gemycircularvirus GeTz1 OX=1792832 PE=4 SV=1
MM1 pKa = 7.67SSFKK5 pKa = 10.61FQSRR9 pKa = 11.84YY10 pKa = 10.0VLLTYY15 pKa = 7.32PQCGDD20 pKa = 3.73LDD22 pKa = 3.62PWAVSDD28 pKa = 4.22HH29 pKa = 6.39LSSPRR34 pKa = 11.84AEE36 pKa = 4.25CIVGRR41 pKa = 11.84EE42 pKa = 3.92VHH44 pKa = 6.69RR45 pKa = 11.84DD46 pKa = 3.27GGIHH50 pKa = 5.95LHH52 pKa = 6.54CFADD56 pKa = 4.13FGKK59 pKa = 10.45RR60 pKa = 11.84FSSRR64 pKa = 11.84NTKK67 pKa = 9.62IFDD70 pKa = 3.7VGGHH74 pKa = 6.45HH75 pKa = 7.52PNTSPSKK82 pKa = 8.91GRR84 pKa = 11.84PGVGYY89 pKa = 10.3DD90 pKa = 3.44YY91 pKa = 10.78AIKK94 pKa = 10.9DD95 pKa = 3.49GDD97 pKa = 4.01VVAEE101 pKa = 4.03GLGRR105 pKa = 11.84PGGDD109 pKa = 3.02GDD111 pKa = 4.71QEE113 pKa = 4.19KK114 pKa = 10.8LPTNAEE120 pKa = 3.46RR121 pKa = 11.84WAEE124 pKa = 3.96IVAAEE129 pKa = 4.07SAEE132 pKa = 4.07EE133 pKa = 3.63FLEE136 pKa = 4.55YY137 pKa = 9.94YY138 pKa = 9.04WRR140 pKa = 11.84RR141 pKa = 11.84LDD143 pKa = 3.35PSAMVRR149 pKa = 11.84SFTQCRR155 pKa = 11.84AIRR158 pKa = 11.84RR159 pKa = 11.84ASLPSTFRR167 pKa = 11.84KK168 pKa = 6.91PTKK171 pKa = 10.26FSDD174 pKa = 3.07RR175 pKa = 11.84SIIRR179 pKa = 11.84NLQEE183 pKa = 3.35WRR185 pKa = 11.84DD186 pKa = 3.8SVNGLKK192 pKa = 10.6VILWGHH198 pKa = 5.33TGGGKK203 pKa = 10.31LSCPALATSLVLWGPSRR220 pKa = 11.84LGGTLWARR228 pKa = 11.84SLGNHH233 pKa = 7.12AYY235 pKa = 10.38FGGLFSMEE243 pKa = 3.77EE244 pKa = 4.43DD245 pKa = 3.21ITNVGYY251 pKa = 10.81AVFDD255 pKa = 4.95DD256 pKa = 3.5IGGLKK261 pKa = 10.18FLSTYY266 pKa = 10.55KK267 pKa = 10.47FWLSHH272 pKa = 4.5QKK274 pKa = 10.24EE275 pKa = 4.64FYY277 pKa = 9.5VTDD280 pKa = 3.94RR281 pKa = 11.84YY282 pKa = 10.46KK283 pKa = 11.02GKK285 pKa = 10.69KK286 pKa = 8.27LVQWGEE292 pKa = 3.48PAIWVNNTDD301 pKa = 3.03PRR303 pKa = 11.84EE304 pKa = 3.86EE305 pKa = 3.82HH306 pKa = 6.87GIRR309 pKa = 11.84DD310 pKa = 4.61EE311 pKa = 4.55EE312 pKa = 4.72IEE314 pKa = 4.15WLNANCQFVYY324 pKa = 10.15IGEE327 pKa = 4.41SIII330 pKa = 4.25

Molecular weight: 37.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A125R3J9|A0A125R3J9_9VIRU Uncharacterized protein OS=Human gemycircularvirus GeTz1 OX=1792832 PE=4 SV=1
MM1 pKa = 7.5SCLPTPNVEE10 pKa = 4.24TSTHH14 pKa = 5.67GLSLTTYY21 pKa = 9.84RR22 pKa = 11.84RR23 pKa = 11.84HH24 pKa = 5.8EE25 pKa = 4.32LSASWEE31 pKa = 4.19EE32 pKa = 4.21KK33 pKa = 9.96FIAMAEE39 pKa = 4.17FTSTVLQTLEE49 pKa = 3.98NDD51 pKa = 3.96SVRR54 pKa = 11.84GTQKK58 pKa = 10.33YY59 pKa = 10.57SMWEE63 pKa = 3.78DD64 pKa = 4.21TIRR67 pKa = 11.84TPALARR73 pKa = 11.84AGLGLDD79 pKa = 4.26MIMQSKK85 pKa = 9.14MEE87 pKa = 4.14MLSLKK92 pKa = 10.72DD93 pKa = 3.38LVAPEE98 pKa = 3.97EE99 pKa = 4.15MGTRR103 pKa = 11.84RR104 pKa = 11.84SFQQMLRR111 pKa = 11.84DD112 pKa = 4.04GLKK115 pKa = 10.23LWLQKK120 pKa = 8.53VQKK123 pKa = 10.23SFWSTIGGDD132 pKa = 3.58WILVRR137 pKa = 11.84WYY139 pKa = 10.21DD140 pKa = 3.48PSHH143 pKa = 6.33NAEE146 pKa = 4.02QYY148 pKa = 10.08AEE150 pKa = 3.88HH151 pKa = 7.7RR152 pKa = 11.84YY153 pKa = 9.85RR154 pKa = 11.84PLSGNLQNSPTGVLFGTFKK173 pKa = 11.02SGGTQQ178 pKa = 2.85

Molecular weight: 20.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

744

178

330

248.0

27.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.317 ± 0.546

1.21 ± 0.326

5.914 ± 0.656

5.242 ± 1.077

4.032 ± 0.611

8.737 ± 0.835

2.419 ± 0.354

4.704 ± 0.575

4.704 ± 0.081

7.527 ± 1.437

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.285 ± 0.905

4.839 ± 1.45

3.763 ± 0.522

3.36 ± 0.701

6.317 ± 0.573

8.871 ± 0.535

7.527 ± 1.837

5.242 ± 0.403

3.091 ± 0.219

3.898 ± 0.49
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski