Butyricicoccus pullicaecorum 1.2

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Butyricicoccus; Butyricicoccus pullicaecorum

Average proteome isoelectric point is 6.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3107 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R8VUF9|R8VUF9_9CLOT Cys_rich_VLP domain-containing protein OS=Butyricicoccus pullicaecorum 1.2 OX=1203606 GN=HMPREF1526_02220 PE=4 SV=1
MM1 pKa = 7.8KK2 pKa = 10.25KK3 pKa = 10.24RR4 pKa = 11.84FWACFLSLSLVLTMMPTMAFAADD27 pKa = 4.03DD28 pKa = 4.06EE29 pKa = 4.97VNEE32 pKa = 4.27PGGTVEE38 pKa = 4.21VCVEE42 pKa = 4.21TEE44 pKa = 3.95GCTLEE49 pKa = 4.75AGHH52 pKa = 6.51EE53 pKa = 4.55GEE55 pKa = 4.53CVVVEE60 pKa = 4.4SEE62 pKa = 4.26PEE64 pKa = 4.0EE65 pKa = 4.64PEE67 pKa = 4.05SAPCKK72 pKa = 8.55VTEE75 pKa = 4.32GCTLEE80 pKa = 4.35NGHH83 pKa = 6.37EE84 pKa = 4.54GEE86 pKa = 4.51CVVAEE91 pKa = 4.41SEE93 pKa = 4.33PEE95 pKa = 3.94EE96 pKa = 4.62PEE98 pKa = 4.47SVPCTVTEE106 pKa = 4.35GCTLEE111 pKa = 5.22DD112 pKa = 3.59GHH114 pKa = 7.05EE115 pKa = 4.57GGCVVEE121 pKa = 4.9SSVDD125 pKa = 3.51SLSEE129 pKa = 4.01DD130 pKa = 3.61EE131 pKa = 5.23EE132 pKa = 4.36IVISITEE139 pKa = 3.96ADD141 pKa = 3.78MNSADD146 pKa = 4.8EE147 pKa = 4.39EE148 pKa = 4.31NPPIKK153 pKa = 10.39SAIARR158 pKa = 11.84LSLADD163 pKa = 4.02PSSVTQLKK171 pKa = 10.08VVTEE175 pKa = 4.31GEE177 pKa = 4.53AYY179 pKa = 10.75LSPEE183 pKa = 4.0DD184 pKa = 3.52NEE186 pKa = 4.47YY187 pKa = 10.67IKK189 pKa = 10.0TVFSSLNYY197 pKa = 9.97LDD199 pKa = 5.45EE200 pKa = 4.95SEE202 pKa = 4.89CQCSTRR208 pKa = 11.84KK209 pKa = 8.44LTEE212 pKa = 4.31AYY214 pKa = 9.75LGKK217 pKa = 10.46SGLPDD222 pKa = 3.05EE223 pKa = 5.66LYY225 pKa = 11.15AEE227 pKa = 4.63FGGLSGYY234 pKa = 9.26TSLEE238 pKa = 3.87TLILPEE244 pKa = 3.91EE245 pKa = 4.34TQVVNSYY252 pKa = 8.46TLSEE256 pKa = 4.27RR257 pKa = 11.84GGGVNDD263 pKa = 3.86TGLTEE268 pKa = 5.56LNLSNNILVIEE279 pKa = 4.39SGAFSGNTNFVGNLVIPDD297 pKa = 3.59SVVFIGNNSFGVGNEE312 pKa = 4.73LIACGTLTLGDD323 pKa = 3.79SVKK326 pKa = 11.13YY327 pKa = 10.23IDD329 pKa = 4.39ANGFKK334 pKa = 10.57SRR336 pKa = 11.84LFEE339 pKa = 4.89GDD341 pKa = 3.75LVIPNSVEE349 pKa = 4.13MINNWSFSPGAFKK362 pKa = 11.05NGTWTLGDD370 pKa = 3.48NLINIGSAALKK381 pKa = 9.43GICSGNSGTFIVLNQYY397 pKa = 10.66KK398 pKa = 9.63MSTQAFADD406 pKa = 3.49NSFEE410 pKa = 4.42KK411 pKa = 10.45IAFEE415 pKa = 4.73EE416 pKa = 5.46GITDD420 pKa = 3.54ISTSQVIASSKK431 pKa = 9.94NIEE434 pKa = 4.36EE435 pKa = 4.08IVLPSTLEE443 pKa = 4.32SISGDD448 pKa = 3.71SAFAGCPALTTINFPKK464 pKa = 10.41SLEE467 pKa = 4.07RR468 pKa = 11.84LGDD471 pKa = 3.45RR472 pKa = 11.84TFEE475 pKa = 4.26NDD477 pKa = 3.29DD478 pKa = 3.66ALTSVVLPSNLTQIGSFTFEE498 pKa = 4.2DD499 pKa = 3.8SGILGLYY506 pKa = 9.52IPEE509 pKa = 4.27SVTDD513 pKa = 3.21IGNRR517 pKa = 11.84IVQDD521 pKa = 3.48IPSGSIVYY529 pKa = 8.81VANEE533 pKa = 3.65ALLNMMNEE541 pKa = 4.74SDD543 pKa = 3.91SAWNNKK549 pKa = 8.03YY550 pKa = 10.84NNEE553 pKa = 3.92TTALAMTNGGSFKK566 pKa = 10.67EE567 pKa = 3.99DD568 pKa = 3.37TEE570 pKa = 4.43FSEE573 pKa = 5.47SVLATPIKK581 pKa = 10.2SGNIFAGWYY590 pKa = 9.96ADD592 pKa = 3.87EE593 pKa = 4.49EE594 pKa = 4.37LADD597 pKa = 3.9EE598 pKa = 4.6VVTNADD604 pKa = 3.4AGNTYY609 pKa = 9.4YY610 pKa = 11.13AEE612 pKa = 4.19WLPSNYY618 pKa = 9.25TVTEE622 pKa = 4.25NINFGSVVYY631 pKa = 10.39GSTASRR637 pKa = 11.84VIQVNGSDD645 pKa = 3.29VAGALKK651 pKa = 10.77VAGNDD656 pKa = 3.09IFDD659 pKa = 3.95TVVNEE664 pKa = 4.43DD665 pKa = 3.1NTITVTPHH673 pKa = 6.06SNLSVGEE680 pKa = 4.13YY681 pKa = 10.88EE682 pKa = 3.82EE683 pKa = 4.27TLYY686 pKa = 9.85VTTPDD691 pKa = 3.09NATFFVPVTLEE702 pKa = 3.95VVPASSKK709 pKa = 11.17LEE711 pKa = 3.52IWANGAKK718 pKa = 10.07KK719 pKa = 10.44LSLSGGGNVTLTITGDD735 pKa = 3.6LNADD739 pKa = 3.76EE740 pKa = 4.67VTVTCNNEE748 pKa = 4.1NIEE751 pKa = 4.25VTKK754 pKa = 11.0NADD757 pKa = 3.17DD758 pKa = 4.36TYY760 pKa = 11.21TVTLPNEE767 pKa = 3.98TADD770 pKa = 3.64YY771 pKa = 9.53TFTATYY777 pKa = 10.67DD778 pKa = 3.31GDD780 pKa = 4.15QNHH783 pKa = 5.92EE784 pKa = 4.22MARR787 pKa = 11.84ATCTVSVTRR796 pKa = 11.84HH797 pKa = 4.98TGGGGASHH805 pKa = 7.74PEE807 pKa = 3.97AGDD810 pKa = 3.45NSSSDD815 pKa = 3.44RR816 pKa = 11.84NDD818 pKa = 3.92RR819 pKa = 11.84DD820 pKa = 4.52DD821 pKa = 6.38DD822 pKa = 3.69DD823 pKa = 4.39TEE825 pKa = 5.53NIDD828 pKa = 4.16EE829 pKa = 4.52EE830 pKa = 4.78DD831 pKa = 3.72VPLTEE836 pKa = 5.25GKK838 pKa = 10.3VADD841 pKa = 4.64FDD843 pKa = 5.64DD844 pKa = 4.63VPADD848 pKa = 3.01AWFAEE853 pKa = 4.28AVQYY857 pKa = 10.99VYY859 pKa = 10.98EE860 pKa = 4.92HH861 pKa = 7.26DD862 pKa = 4.22LMTGVSEE869 pKa = 5.3NLFAPNAQMNRR880 pKa = 11.84AMVAQILFNVEE891 pKa = 3.7KK892 pKa = 10.07PADD895 pKa = 3.95TEE897 pKa = 3.97APAAFRR903 pKa = 11.84DD904 pKa = 3.87VAPDD908 pKa = 2.71AWYY911 pKa = 10.76AEE913 pKa = 4.21AVNWAVWQGYY923 pKa = 6.42MSGYY927 pKa = 9.67GAGSFGPNDD936 pKa = 3.53ALTRR940 pKa = 11.84EE941 pKa = 4.14QLVTVLWRR949 pKa = 11.84YY950 pKa = 9.76SGSPVMGDD958 pKa = 2.69SSMLNTFSDD967 pKa = 3.82AALTSDD973 pKa = 3.92YY974 pKa = 10.87AQQAMIWAYY983 pKa = 9.61AQGVISGNADD993 pKa = 3.14GTLNPQGTATRR1004 pKa = 11.84AEE1006 pKa = 4.18IAQMLMNYY1014 pKa = 9.52CEE1016 pKa = 4.09NVKK1019 pKa = 10.63

Molecular weight: 109.17 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R8VXU9|R8VXU9_9CLOT Uncharacterized protein OS=Butyricicoccus pullicaecorum 1.2 OX=1203606 GN=HMPREF1526_02727 PE=4 SV=1
MM1 pKa = 7.17VKK3 pKa = 10.19GVSKK7 pKa = 10.17RR8 pKa = 11.84VVVVRR13 pKa = 11.84PDD15 pKa = 3.2DD16 pKa = 3.69SRR18 pKa = 11.84FFEE21 pKa = 3.92QAFFIVRR28 pKa = 11.84EE29 pKa = 4.18GRR31 pKa = 11.84TASGDD36 pKa = 3.69ALRR39 pKa = 11.84EE40 pKa = 4.04ACALAARR47 pKa = 11.84YY48 pKa = 7.74HH49 pKa = 6.48AKK51 pKa = 10.37HH52 pKa = 6.51PARR55 pKa = 11.84PLPLRR60 pKa = 11.84RR61 pKa = 11.84YY62 pKa = 6.59TAPQMVLSALFGSGLMGAAWAVSCLVFF89 pKa = 5.28

Molecular weight: 9.78 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3107

0

3107

955345

30

3162

307.5

34.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.491 ± 0.056

1.713 ± 0.02

5.93 ± 0.041

6.507 ± 0.052

3.78 ± 0.032

7.482 ± 0.041

2.012 ± 0.024

6.2 ± 0.041

4.831 ± 0.046

9.474 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.874 ± 0.027

3.429 ± 0.029

4.034 ± 0.025

3.876 ± 0.031

5.385 ± 0.047

5.52 ± 0.037

5.938 ± 0.042

7.034 ± 0.04

1.0 ± 0.014

3.49 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski