Cucumis sativus (Cucumber)

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; fabids; Cucurbitales; Cucurbitaceae; Benincaseae; Cucumis

Average proteome isoelectric point is 6.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 23744 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A0K5N6|A0A0A0K5N6_CUCSA Pep3_Vps18 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G046700 PE=3 SV=1
MM1 pKa = 7.83EE2 pKa = 6.84DD3 pKa = 4.3DD4 pKa = 5.02NIDD7 pKa = 3.74NGFEE11 pKa = 4.15DD12 pKa = 5.27AMPDD16 pKa = 3.48VNQPDD21 pKa = 3.24IGDD24 pKa = 4.2PNNHH28 pKa = 5.66FMDD31 pKa = 4.02EE32 pKa = 4.25ATCFEE37 pKa = 4.39NEE39 pKa = 3.89KK40 pKa = 10.31HH41 pKa = 6.5DD42 pKa = 4.38AAAHH46 pKa = 6.0FDD48 pKa = 3.43NGEE51 pKa = 3.91AYY53 pKa = 9.91EE54 pKa = 4.33PEE56 pKa = 4.46FPDD59 pKa = 3.95SRR61 pKa = 11.84SSLEE65 pKa = 3.96DD66 pKa = 2.98LCRR69 pKa = 11.84SHH71 pKa = 6.96LVSDD75 pKa = 3.86ICGLITT81 pKa = 4.56

Molecular weight: 9.08 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A0KV58|A0A0A0KV58_CUCSA PCI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G054300 PE=3 SV=1
MM1 pKa = 7.43HH2 pKa = 8.24LLRR5 pKa = 11.84HH6 pKa = 6.09HH7 pKa = 6.85PTPLMLHH14 pKa = 6.26RR15 pKa = 11.84RR16 pKa = 11.84RR17 pKa = 11.84RR18 pKa = 11.84HH19 pKa = 4.67PTPPTLHH26 pKa = 6.79LHH28 pKa = 5.81RR29 pKa = 11.84HH30 pKa = 5.58HH31 pKa = 6.92PTPLTLHH38 pKa = 6.55LHH40 pKa = 5.67RR41 pKa = 11.84HH42 pKa = 5.36HH43 pKa = 7.27PAPLTLRR50 pKa = 11.84LHH52 pKa = 6.69RR53 pKa = 11.84HH54 pKa = 5.59HH55 pKa = 7.42PMPPTLRR62 pKa = 11.84LHH64 pKa = 6.4RR65 pKa = 11.84HH66 pKa = 5.42HH67 pKa = 7.38PAPLTLRR74 pKa = 11.84LHH76 pKa = 6.39RR77 pKa = 11.84HH78 pKa = 5.98HH79 pKa = 7.17PTQLTLPHH87 pKa = 6.72PHH89 pKa = 7.73PIQPTPHH96 pKa = 6.18HH97 pKa = 6.47HH98 pKa = 6.72HH99 pKa = 7.63LIPLLTYY106 pKa = 9.65HH107 pKa = 6.38LHH109 pKa = 7.25RR110 pKa = 11.84RR111 pKa = 11.84LTWVHH116 pKa = 5.66LHH118 pKa = 6.2HH119 pKa = 6.96RR120 pKa = 11.84RR121 pKa = 11.84QRR123 pKa = 11.84LMEE126 pKa = 4.18HH127 pKa = 6.92

Molecular weight: 15.64 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

23744

0

23744

8735437

15

5074

367.9

41.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.472 ± 0.013

1.835 ± 0.008

5.203 ± 0.011

6.44 ± 0.02

4.448 ± 0.011

6.466 ± 0.019

2.405 ± 0.008

5.476 ± 0.012

6.125 ± 0.018

9.721 ± 0.02

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.405 ± 0.007

4.603 ± 0.011

4.948 ± 0.018

3.563 ± 0.011

5.314 ± 0.014

9.294 ± 0.019

4.868 ± 0.01

6.403 ± 0.012

1.271 ± 0.006

2.739 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski