Bacilli bacterium VT-13-104

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; unclassified Bacilli

Average proteome isoelectric point is 6.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3231 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0K0GBD2|A0A0K0GBD2_9FIRM 3-mercaptopyruvate sulfurtransferase OS=Bacilli bacterium VT-13-104 OX=1637974 GN=WH51_07045 PE=4 SV=1
MM1 pKa = 7.19AQSNQTAGKK10 pKa = 8.76YY11 pKa = 8.85VLSIAFVSSLVFTPVLSGKK30 pKa = 9.71VFANAGADD38 pKa = 3.37ASEE41 pKa = 3.95GDD43 pKa = 3.51ITISEE48 pKa = 4.55SNLSNIQEE56 pKa = 4.12QEE58 pKa = 4.16VASSSEE64 pKa = 3.82GTLIQHH70 pKa = 6.81GDD72 pKa = 2.91VGAAVEE78 pKa = 5.08DD79 pKa = 3.84IQKK82 pKa = 10.07EE83 pKa = 4.18LQEE86 pKa = 4.1QGYY89 pKa = 7.36YY90 pKa = 10.11TYY92 pKa = 10.21TIDD95 pKa = 5.45GIFGSITGQAVRR107 pKa = 11.84DD108 pKa = 3.96YY109 pKa = 11.08QADD112 pKa = 3.01HH113 pKa = 6.83HH114 pKa = 6.69LQVDD118 pKa = 5.22GIVGPNTLEE127 pKa = 4.01TMALPEE133 pKa = 4.32MEE135 pKa = 4.35SSEE138 pKa = 4.52DD139 pKa = 3.37NLTITEE145 pKa = 4.22IDD147 pKa = 3.48SSEE150 pKa = 4.53DD151 pKa = 3.24NLTVTEE157 pKa = 4.5SSSEE161 pKa = 3.9DD162 pKa = 3.32VSNIKK167 pKa = 10.41SDD169 pKa = 3.03IVAVAKK175 pKa = 10.51SVIGTPYY182 pKa = 9.67VWGGTTTEE190 pKa = 4.67GMDD193 pKa = 3.1SSGFINYY200 pKa = 8.1VFNQVGIDD208 pKa = 3.98DD209 pKa = 3.84ISRR212 pKa = 11.84THH214 pKa = 7.72RR215 pKa = 11.84EE216 pKa = 3.24MWEE219 pKa = 3.67NDD221 pKa = 3.22GNYY224 pKa = 9.56VDD226 pKa = 4.98HH227 pKa = 7.4PSIGDD232 pKa = 3.39VVFFEE237 pKa = 4.46GTYY240 pKa = 8.58DD241 pKa = 3.53TEE243 pKa = 4.64GASHH247 pKa = 6.39SGVYY251 pKa = 9.75IGNNQMIHH259 pKa = 6.69AGDD262 pKa = 4.66DD263 pKa = 3.93GVSVADD269 pKa = 3.65ISIDD273 pKa = 3.39YY274 pKa = 7.82WQSHH278 pKa = 4.92YY279 pKa = 11.17LGAKK283 pKa = 9.83SFTEE287 pKa = 4.07

Molecular weight: 30.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0K0ILE5|A0A0K0ILE5_9FIRM Lipoate--protein ligase OS=Bacilli bacterium VT-13-104 OX=1637974 GN=WH51_16490 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 9.6RR3 pKa = 11.84TFQPNNRR10 pKa = 11.84KK11 pKa = 9.23RR12 pKa = 11.84KK13 pKa = 8.22KK14 pKa = 8.69VHH16 pKa = 5.46GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTKK25 pKa = 10.19NGRR28 pKa = 11.84KK29 pKa = 8.49VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.05GRR39 pKa = 11.84KK40 pKa = 8.7VLSAA44 pKa = 4.05

Molecular weight: 5.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3231

0

3231

926547

26

1430

286.8

32.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.409 ± 0.04

0.632 ± 0.013

5.246 ± 0.039

7.774 ± 0.061

4.559 ± 0.035

6.762 ± 0.04

2.034 ± 0.019

8.501 ± 0.05

7.272 ± 0.044

9.438 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.809 ± 0.021

4.888 ± 0.032

3.507 ± 0.022

3.544 ± 0.03

3.866 ± 0.03

5.918 ± 0.028

5.386 ± 0.026

6.91 ± 0.038

0.959 ± 0.016

3.583 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski