Candidatus Hodgkinia cicadicola

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Rhizobiales incertae sedis; Candidatus Hodgkinia

Average proteome isoelectric point is 8.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 252 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2G9FSE3|A0A2G9FSE3_9RHIZ Phosphoribosyl-ATP diphosphatase OS=Candidatus Hodgkinia cicadicola OX=573658 GN=hisI PE=3 SV=1
MM1 pKa = 6.91FTGLVKK7 pKa = 9.95TLGMIVDD14 pKa = 4.22VLPLSGGIKK23 pKa = 10.18LCIISKK29 pKa = 10.13LRR31 pKa = 11.84LNVGNSISCSGVCLTVVSVNEE52 pKa = 4.0YY53 pKa = 9.02YY54 pKa = 10.81FEE56 pKa = 4.47VEE58 pKa = 3.96VWSEE62 pKa = 4.17SLCLSGLVDD71 pKa = 4.1LQRR74 pKa = 11.84FDD76 pKa = 3.56MVNLEE81 pKa = 4.25EE82 pKa = 5.74PITLNTPLHH91 pKa = 6.03GNITNGYY98 pKa = 7.85SKK100 pKa = 10.97SVVTIDD106 pKa = 2.99KK107 pKa = 9.62TYY109 pKa = 10.86VYY111 pKa = 10.02GDD113 pKa = 3.84SIILVIEE120 pKa = 4.2CPKK123 pKa = 9.87WISDD127 pKa = 4.14EE128 pKa = 4.07ITSPSSISLDD138 pKa = 3.41GVALTVTRR146 pKa = 11.84SGEE149 pKa = 4.03DD150 pKa = 3.36YY151 pKa = 11.32FEE153 pKa = 4.41VLLTRR158 pKa = 11.84YY159 pKa = 7.81TILNTTFKK167 pKa = 11.17YY168 pKa = 10.81FDD170 pKa = 3.4TKK172 pKa = 10.45RR173 pKa = 11.84WFSLEE178 pKa = 3.75QDD180 pKa = 3.67VSQRR184 pKa = 11.84WCC186 pKa = 3.47

Molecular weight: 20.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2G9FSF5|A0A2G9FSF5_9RHIZ Electron transfer flavoprotein large subunit OS=Candidatus Hodgkinia cicadicola OX=573658 GN=etfA PE=4 SV=1
MM1 pKa = 8.07RR2 pKa = 11.84YY3 pKa = 9.25NVQINVRR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84PWRR15 pKa = 11.84SWSCFGLYY23 pKa = 10.21RR24 pKa = 11.84LIDD27 pKa = 3.71LVSIKK32 pKa = 10.53SQILSDD38 pKa = 3.63RR39 pKa = 11.84NSWITEE45 pKa = 4.09LLTTRR50 pKa = 11.84PWFIFKK56 pKa = 10.4RR57 pKa = 11.84IKK59 pKa = 9.84EE60 pKa = 4.16EE61 pKa = 4.21QMEE64 pKa = 4.23LTLAVNFQVNRR75 pKa = 11.84KK76 pKa = 8.75IVIEE80 pKa = 4.5SIDD83 pKa = 4.66LILQVFLLARR93 pKa = 11.84RR94 pKa = 11.84IGLNINNVIRR104 pKa = 11.84LIDD107 pKa = 3.45YY108 pKa = 8.41WNIVASTQRR117 pKa = 11.84FNKK120 pKa = 8.46WSNFSRR126 pKa = 11.84PNKK129 pKa = 10.21SNYY132 pKa = 5.87NTNYY136 pKa = 9.35RR137 pKa = 11.84SSMNNFRR144 pKa = 11.84SNRR147 pKa = 11.84LDD149 pKa = 4.03NITLSRR155 pKa = 11.84LNNSISNLFMVITNLRR171 pKa = 11.84TGSYY175 pKa = 9.86RR176 pKa = 11.84YY177 pKa = 10.18RR178 pKa = 11.84NILNGLFYY186 pKa = 11.12NVLYY190 pKa = 10.96NIITLICNRR199 pKa = 11.84SIKK202 pKa = 10.45YY203 pKa = 10.74SMVINEE209 pKa = 5.35IIDD212 pKa = 3.42KK213 pKa = 10.38MEE215 pKa = 3.79

Molecular weight: 25.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

252

0

252

101011

41

1395

400.8

45.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.181 ± 0.107

2.308 ± 0.056

4.682 ± 0.126

4.196 ± 0.097

3.778 ± 0.094

5.518 ± 0.133

2.057 ± 0.044

10.031 ± 0.164

7.942 ± 0.127

10.854 ± 0.111

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.427 ± 0.051

7.646 ± 0.119

3.063 ± 0.058

2.715 ± 0.065

4.541 ± 0.089

7.608 ± 0.106

5.958 ± 0.115

6.611 ± 0.146

1.215 ± 0.046

3.671 ± 0.079
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski