Shewanella denitrificans (strain OS217 / ATCC BAA-1090 / DSM 15013)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales; Shewanellaceae; Shewanella; Shewanella denitrificans

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3727 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q12JY1|Q12JY1_SHEDO Peptidase C39 bacteriocin processing OS=Shewanella denitrificans (strain OS217 / ATCC BAA-1090 / DSM 15013) OX=318161 GN=Sden_2967 PE=4 SV=1
MM1 pKa = 7.6KK2 pKa = 10.46KK3 pKa = 8.89LTLALSIAAIMSSSAYY19 pKa = 9.83ADD21 pKa = 3.16GMTEE25 pKa = 3.9SDD27 pKa = 4.36SSVDD31 pKa = 3.27LDD33 pKa = 4.12KK34 pKa = 11.07KK35 pKa = 10.71VKK37 pKa = 8.21VTKK40 pKa = 9.86TLDD43 pKa = 3.3YY44 pKa = 10.81EE45 pKa = 4.58GTVTINGNINVSGLGMAVVEE65 pKa = 4.33NVQEE69 pKa = 4.19SSMNEE74 pKa = 3.7VGNYY78 pKa = 8.11YY79 pKa = 10.14HH80 pKa = 7.64DD81 pKa = 4.39NDD83 pKa = 4.76AHH85 pKa = 5.75ITDD88 pKa = 3.91NAFEE92 pKa = 4.19NASGNIGVNQASGDD106 pKa = 3.79FNAQTNSAALASIDD120 pKa = 3.69ANFAFGSGDD129 pKa = 3.49AEE131 pKa = 3.99IFSTQVGGFNGVWNEE146 pKa = 3.54ASTNTATIDD155 pKa = 3.44GASFMNATGNIGVNIAAGNNNLQANNMAASAYY187 pKa = 10.39SGALGEE193 pKa = 4.35ASVSNVQQTGHH204 pKa = 6.6NGTMNMSVVEE214 pKa = 4.4DD215 pKa = 3.46SVEE218 pKa = 4.35FININLALNADD229 pKa = 3.77GSYY232 pKa = 10.94DD233 pKa = 3.54GSSGLAEE240 pKa = 4.29PYY242 pKa = 9.36YY243 pKa = 9.88PQVILEE249 pKa = 4.22GDD251 pKa = 3.61GGHH254 pKa = 6.41NSGDD258 pKa = 3.56GTVQGHH264 pKa = 6.69IDD266 pKa = 3.97FDD268 pKa = 4.42TEE270 pKa = 4.09NPTGSSVMTFNEE282 pKa = 4.09EE283 pKa = 4.47GEE285 pKa = 4.25LDD287 pKa = 3.58LAGTVSGALPFFIQTVAQKK306 pKa = 9.49TNNSTLLAGTAFQNSAGNIGVNMASGTGNLQSNNLALTSITAASDD351 pKa = 3.6VIEE354 pKa = 4.17PP355 pKa = 3.99

Molecular weight: 36.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q12HM9|MNME_SHEDO tRNA modification GTPase MnmE OS=Shewanella denitrificans (strain OS217 / ATCC BAA-1090 / DSM 15013) OX=318161 GN=mnmE PE=3 SV=2
MM1 pKa = 7.69SKK3 pKa = 9.01RR4 pKa = 11.84TFQPSNLKK12 pKa = 10.14RR13 pKa = 11.84KK14 pKa = 9.13RR15 pKa = 11.84SHH17 pKa = 6.17GFRR20 pKa = 11.84ARR22 pKa = 11.84MATVGGRR29 pKa = 11.84KK30 pKa = 9.01VLARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.39GRR40 pKa = 11.84ARR42 pKa = 11.84LSAA45 pKa = 3.91

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3727

0

3727

1268806

36

5236

340.4

37.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.289 ± 0.039

1.013 ± 0.014

5.478 ± 0.038

5.639 ± 0.038

4.022 ± 0.028

6.798 ± 0.045

2.27 ± 0.023

6.193 ± 0.041

5.184 ± 0.035

10.929 ± 0.062

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.54 ± 0.026

4.301 ± 0.034

3.882 ± 0.024

5.014 ± 0.041

4.313 ± 0.034

6.965 ± 0.035

5.335 ± 0.054

6.64 ± 0.042

1.187 ± 0.013

3.006 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski