Shimia gijangensis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Roseobacteraceae; Shimia

Average proteome isoelectric point is 6.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4001 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1M6PQK2|A0A1M6PQK2_9RHOB 50S ribosomal subunit assembly factor BipA OS=Shimia gijangensis OX=1470563 GN=bipA PE=3 SV=1
MM1 pKa = 7.6TGNKK5 pKa = 8.74MNNLALKK12 pKa = 9.05TGLAAAFLFGLSGLASACTGVEE34 pKa = 4.13DD35 pKa = 3.88NAPEE39 pKa = 3.9ISEE42 pKa = 4.56TYY44 pKa = 10.48VDD46 pKa = 3.47EE47 pKa = 4.62TGQFHH52 pKa = 7.3AVGADD57 pKa = 2.85GWLTFGLGEE66 pKa = 3.86QLVYY70 pKa = 8.87FVCLVDD76 pKa = 4.51NEE78 pKa = 4.02NDD80 pKa = 3.38YY81 pKa = 11.6LIAQNGSANDD91 pKa = 3.55EE92 pKa = 4.21DD93 pKa = 4.65YY94 pKa = 11.39EE95 pKa = 4.45LYY97 pKa = 10.89SRR99 pKa = 11.84FEE101 pKa = 3.97WFVVEE106 pKa = 5.3DD107 pKa = 3.84QLFFCNQVSDD117 pKa = 4.35AGSQADD123 pKa = 3.72AMDD126 pKa = 4.72FAALPRR132 pKa = 11.84ADD134 pKa = 3.59QANPTLRR141 pKa = 11.84GCGVDD146 pKa = 3.63SQAWSALRR154 pKa = 11.84VVKK157 pKa = 10.18PP158 pKa = 3.61

Molecular weight: 17.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1M6MBU3|A0A1M6MBU3_9RHOB Cytochrome P450 OS=Shimia gijangensis OX=1470563 GN=SAMN05444000_11399 PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 10.31QPSNLVRR12 pKa = 11.84KK13 pKa = 8.96RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.62GFRR19 pKa = 11.84SRR21 pKa = 11.84MATKK25 pKa = 10.45AGRR28 pKa = 11.84KK29 pKa = 8.81IINARR34 pKa = 11.84RR35 pKa = 11.84AHH37 pKa = 5.43GRR39 pKa = 11.84KK40 pKa = 9.31SLSAA44 pKa = 3.91

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4001

0

4001

1224542

26

9654

306.1

33.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.985 ± 0.048

0.961 ± 0.015

6.153 ± 0.045

5.991 ± 0.041

3.93 ± 0.026

8.488 ± 0.048

2.145 ± 0.019

5.382 ± 0.029

3.855 ± 0.033

9.713 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.941 ± 0.028

3.043 ± 0.025

4.749 ± 0.032

3.323 ± 0.022

6.057 ± 0.047

5.629 ± 0.038

5.553 ± 0.04

7.356 ± 0.033

1.406 ± 0.017

2.34 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski