Rhizobium etli

Taxonomy: cellular organisms;

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6373 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A327XDZ5|A0A327XDZ5_RHIET Diguanylate cyclase (GGDEF)-like protein OS=Rhizobium etli OX=29449 GN=BCL34_11815 PE=4 SV=1
MM1 pKa = 7.3KK2 pKa = 9.79MNYY5 pKa = 8.86GAAAFVAATVGFCPLHH21 pKa = 5.78AAWAEE26 pKa = 4.17VAVNGALFLGATDD39 pKa = 4.58DD40 pKa = 4.86GYY42 pKa = 11.84GNTDD46 pKa = 3.51SEE48 pKa = 4.25IHH50 pKa = 6.85LDD52 pKa = 3.77LNSSGKK58 pKa = 9.81FALSDD63 pKa = 3.47AVTLGFDD70 pKa = 4.15VEE72 pKa = 4.37FDD74 pKa = 3.24VDD76 pKa = 4.45NIDD79 pKa = 5.46DD80 pKa = 3.87VPTQYY85 pKa = 10.55RR86 pKa = 11.84GSLDD90 pKa = 3.69DD91 pKa = 3.53FTVYY95 pKa = 9.94MISGAMVSS103 pKa = 3.44

Molecular weight: 10.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A327Y532|A0A327Y532_RHIET Cystathionine beta-lyase OS=Rhizobium etli OX=29449 GN=BCL34_104127 PE=3 SV=1
NN1 pKa = 6.55RR2 pKa = 11.84HH3 pKa = 5.78HH4 pKa = 7.08RR5 pKa = 11.84VLPPRR10 pKa = 11.84LARR13 pKa = 11.84LNRR16 pKa = 11.84MISKK20 pKa = 9.98RR21 pKa = 11.84RR22 pKa = 11.84AAVEE26 pKa = 4.17TTFATLKK33 pKa = 10.54HH34 pKa = 6.08RR35 pKa = 11.84MGLGLIRR42 pKa = 11.84YY43 pKa = 8.2RR44 pKa = 11.84GLVKK48 pKa = 10.6ARR50 pKa = 11.84AQVLMATIAFNMRR63 pKa = 11.84RR64 pKa = 11.84WVTMATT70 pKa = 3.53

Molecular weight: 8.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6373

0

6373

1901876

29

8395

298.4

32.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.806 ± 0.039

0.779 ± 0.01

5.819 ± 0.029

5.648 ± 0.033

3.88 ± 0.021

8.22 ± 0.037

2.006 ± 0.017

5.777 ± 0.024

3.835 ± 0.03

9.78 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.622 ± 0.018

2.929 ± 0.024

4.777 ± 0.024

3.141 ± 0.019

6.471 ± 0.043

6.011 ± 0.026

5.494 ± 0.051

7.407 ± 0.029

1.277 ± 0.014

2.319 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski