Cryptosporangium phraense

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Cryptosporangiales; Cryptosporangiaceae; Cryptosporangium

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7680 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A545APF9|A0A545APF9_9ACTN sn-glycerol-3-phosphate ABC transporter ATP-binding protein UgpC OS=Cryptosporangium phraense OX=2593070 GN=ugpC PE=4 SV=1
MM1 pKa = 7.49GFGVTLGATVAASLVAVIAAVALLIGAAGTSAVTGTGAGTVMTAAQLEE49 pKa = 4.41NEE51 pKa = 4.3LVDD54 pKa = 3.86NNTNEE59 pKa = 4.34LVTSAHH65 pKa = 7.06CIPEE69 pKa = 4.28GVRR72 pKa = 11.84ADD74 pKa = 3.49GSGTYY79 pKa = 10.56GCVIYY84 pKa = 10.41FSDD87 pKa = 3.76GDD89 pKa = 3.74NRR91 pKa = 11.84GATILVGPDD100 pKa = 3.46GDD102 pKa = 3.59WLEE105 pKa = 4.14NN106 pKa = 3.55

Molecular weight: 10.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A545AXS3|A0A545AXS3_9ACTN Uncharacterized protein OS=Cryptosporangium phraense OX=2593070 GN=FL583_09955 PE=4 SV=1
MM1 pKa = 7.49ARR3 pKa = 11.84TRR5 pKa = 11.84APAGTPTWPGPRR17 pKa = 11.84PGPRR21 pKa = 11.84PGSRR25 pKa = 11.84PGSRR29 pKa = 11.84PGSRR33 pKa = 11.84PGSRR37 pKa = 11.84PGSRR41 pKa = 11.84PGSRR45 pKa = 11.84PGPRR49 pKa = 11.84PGPGPSPRR57 pKa = 11.84PPARR61 pKa = 11.84QQVFSVSSGRR71 pKa = 11.84LRR73 pKa = 3.67

Molecular weight: 7.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7680

0

7680

2457430

27

6327

320.0

34.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.988 ± 0.043

0.668 ± 0.007

6.163 ± 0.024

5.171 ± 0.029

2.791 ± 0.016

9.269 ± 0.03

1.998 ± 0.014

3.475 ± 0.019

1.751 ± 0.019

10.568 ± 0.034

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.528 ± 0.011

1.771 ± 0.012

6.155 ± 0.027

2.586 ± 0.016

8.046 ± 0.033

5.36 ± 0.021

6.033 ± 0.029

9.15 ± 0.03

1.521 ± 0.011

2.01 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski