Escherichia phage Stx2 II

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Sepvirinae; Traversvirus; Escherichia virus Stx2 II

Average proteome isoelectric point is 7.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 170 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q7Y2L9|Q7Y2L9_9CAUD Ea10 protein OS=Escherichia phage Stx2 II OX=194949 GN=ea10 PE=4 SV=1
MM1 pKa = 7.62EE2 pKa = 4.36FHH4 pKa = 7.21EE5 pKa = 5.34SAICDD10 pKa = 3.52FRR12 pKa = 11.84ANANSVKK19 pKa = 9.25PQPIAVLFKK28 pKa = 10.94TMGAWAVLCFAADD41 pKa = 3.61DD42 pKa = 3.83TDD44 pKa = 3.42ARR46 pKa = 11.84MAIGQEE52 pKa = 4.02MEE54 pKa = 3.99MDD56 pKa = 3.55PTNDD60 pKa = 2.86EE61 pKa = 4.58FIIYY65 pKa = 7.71GAPSNYY71 pKa = 10.43LLDD74 pKa = 3.47TCNIYY79 pKa = 10.99NKK81 pKa = 10.37AAA83 pKa = 3.64

Molecular weight: 9.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q7Y2J0|Q7Y2J0_9CAUD Uncharacterized protein OS=Escherichia phage Stx2 II OX=194949 PE=4 SV=1
MM1 pKa = 8.16DD2 pKa = 4.04ILFRR6 pKa = 11.84FVFIAAIHH14 pKa = 5.52RR15 pKa = 11.84LVRR18 pKa = 11.84GFVLLLPEE26 pKa = 4.06IRR28 pKa = 11.84RR29 pKa = 11.84VPAYY33 pKa = 9.46PGNTASRR40 pKa = 11.84ATDD43 pKa = 3.2ALACHH48 pKa = 6.62IRR50 pKa = 11.84QVCTEE55 pKa = 4.93FINQRR60 pKa = 11.84TKK62 pKa = 10.78LCGVIYY68 pKa = 10.56RR69 pKa = 11.84RR70 pKa = 11.84TAKK73 pKa = 10.06IKK75 pKa = 10.46FPLHH79 pKa = 5.25PHH81 pKa = 6.55HH82 pKa = 6.95GKK84 pKa = 8.74QHH86 pKa = 6.28IFRR89 pKa = 11.84ILLQCCFCSTDD100 pKa = 2.88KK101 pKa = 11.39VLIQRR106 pKa = 11.84LVHH109 pKa = 6.12TPAGG113 pKa = 3.6

Molecular weight: 13.0 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

170

0

170

27450

41

2576

161.5

18.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.747 ± 0.328

2.12 ± 0.249

4.696 ± 0.225

5.322 ± 0.268

4.011 ± 0.226

6.521 ± 0.368

2.368 ± 0.153

5.064 ± 0.262

5.016 ± 0.188

8.572 ± 0.243

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.863 ± 0.114

3.887 ± 0.153

5.231 ± 0.236

4.332 ± 0.22

7.596 ± 0.302

7.512 ± 0.332

5.654 ± 0.199

6.368 ± 0.224

1.563 ± 0.114

2.557 ± 0.144
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski