Spiroplasma diminutum CUAS-1

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Tenericutes; Mollicutes; Entomoplasmatales; Spiroplasmataceae; Spiroplasma; Spiroplasma diminutum

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 858 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S5MJ56|S5MJ56_9MOLU Uncharacterized protein OS=Spiroplasma diminutum CUAS-1 OX=1276221 GN=SDIMI_v3c02860 PE=4 SV=1
MM1 pKa = 7.53KK2 pKa = 9.16KK3 pKa = 7.96TWIDD7 pKa = 3.0KK8 pKa = 9.64PMCIGCMACVQIDD21 pKa = 3.66EE22 pKa = 4.7TEE24 pKa = 4.19TLFMDD29 pKa = 4.97DD30 pKa = 5.67DD31 pKa = 4.52GFAEE35 pKa = 4.26ANEE38 pKa = 4.04NDD40 pKa = 4.11LEE42 pKa = 4.25LVEE45 pKa = 4.66CQMVCPTGAVKK56 pKa = 10.39IGDD59 pKa = 3.79EE60 pKa = 4.12

Molecular weight: 6.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S5MDM7|S5MDM7_9MOLU Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B OS=Spiroplasma diminutum CUAS-1 OX=1276221 GN=gatB PE=3 SV=1
MM1 pKa = 7.32KK2 pKa = 9.47RR3 pKa = 11.84TWQPSKK9 pKa = 10.03IKK11 pKa = 10.14HH12 pKa = 5.53ARR14 pKa = 11.84THH16 pKa = 5.78GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.41NGRR28 pKa = 11.84KK29 pKa = 9.2VIKK32 pKa = 10.14ARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.33GRR39 pKa = 11.84AKK41 pKa = 9.64LTAA44 pKa = 4.21

Molecular weight: 5.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

858

0

858

291642

31

1727

339.9

38.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.799 ± 0.094

0.667 ± 0.025

5.401 ± 0.057

7.53 ± 0.081

5.447 ± 0.075

5.099 ± 0.082

1.161 ± 0.029

10.348 ± 0.084

9.852 ± 0.086

9.274 ± 0.073

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.221 ± 0.034

7.679 ± 0.107

2.448 ± 0.038

3.061 ± 0.045

2.906 ± 0.055

6.551 ± 0.065

5.061 ± 0.05

5.566 ± 0.067

0.974 ± 0.028

3.955 ± 0.055
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski