Actinopolymorpha singaporensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Propionibacteriales; Actinopolymorphaceae; Actinopolymorpha

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5596 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H1L117|A0A1H1L117_9ACTN Oligosaccharide biosynthesis protein Alg14 like OS=Actinopolymorpha singaporensis OX=117157 GN=SAMN04489717_0029 PE=4 SV=1
MM1 pKa = 7.79ALTSTYY7 pKa = 8.93TVSGMTCEE15 pKa = 4.12HH16 pKa = 6.67CVAAVTSEE24 pKa = 3.9LKK26 pKa = 10.89AVDD29 pKa = 3.9GVSDD33 pKa = 3.6VSVDD37 pKa = 3.81LVPGGNSTVSVTSSTEE53 pKa = 3.75VSPEE57 pKa = 3.75AVAEE61 pKa = 4.23ALDD64 pKa = 3.7EE65 pKa = 4.81AGDD68 pKa = 3.91YY69 pKa = 11.08RR70 pKa = 11.84LVTSS74 pKa = 4.58

Molecular weight: 7.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H1NF16|A0A1H1NF16_9ACTN N-acetylglucosaminyldiphosphoundecaprenol N-acetyl-beta-D-mannosaminyltransferase OS=Actinopolymorpha singaporensis OX=117157 GN=SAMN04489717_1214 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 9.6RR3 pKa = 11.84TFQPNNRR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84AKK14 pKa = 8.7THH16 pKa = 5.15GFRR19 pKa = 11.84LRR21 pKa = 11.84MRR23 pKa = 11.84TRR25 pKa = 11.84AGRR28 pKa = 11.84AILSARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.31GRR39 pKa = 11.84QRR41 pKa = 11.84LAVV44 pKa = 3.51

Molecular weight: 5.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5596

0

5596

1865002

29

3128

333.3

35.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.101 ± 0.044

0.776 ± 0.009

6.201 ± 0.027

5.437 ± 0.035

2.832 ± 0.02

9.41 ± 0.03

2.263 ± 0.016

3.026 ± 0.019

1.797 ± 0.023

10.153 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.682 ± 0.012

1.786 ± 0.014

6.189 ± 0.028

2.731 ± 0.018

8.58 ± 0.032

5.092 ± 0.026

5.952 ± 0.024

9.18 ± 0.031

1.646 ± 0.015

2.166 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski