Glaciihabitans tibetensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Glaciihabitans

Average proteome isoelectric point is 5.99

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3456 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2T0VCQ4|A0A2T0VCQ4_9MICO Transmembrane protein DUF3566 OS=Glaciihabitans tibetensis OX=1266600 GN=B0I08_105124 PE=4 SV=1
MM1 pKa = 7.38KK2 pKa = 10.26LDD4 pKa = 3.61RR5 pKa = 11.84RR6 pKa = 11.84LVACSALVAAAALLGGCASTPATSTATNPSEE37 pKa = 4.48ADD39 pKa = 3.2AGQSDD44 pKa = 3.83AGEE47 pKa = 4.32TRR49 pKa = 11.84TLPTDD54 pKa = 3.33VQADD58 pKa = 3.84YY59 pKa = 11.3QLGGSYY65 pKa = 9.93EE66 pKa = 4.04PPAGVGLVVRR76 pKa = 11.84DD77 pKa = 3.69SGDD80 pKa = 3.42YY81 pKa = 9.68PAEE84 pKa = 3.93GMYY87 pKa = 10.1SICYY91 pKa = 9.74VNGFQTQPQDD101 pKa = 3.22AAVWLAEE108 pKa = 3.98HH109 pKa = 7.33PEE111 pKa = 4.46LILWEE116 pKa = 4.61GGGRR120 pKa = 11.84AEE122 pKa = 4.51GVDD125 pKa = 3.7GAGQPVIDD133 pKa = 4.87PNWPDD138 pKa = 3.7EE139 pKa = 4.44MILDD143 pKa = 4.37TSTEE147 pKa = 4.25SNRR150 pKa = 11.84TAIAEE155 pKa = 4.44VIGEE159 pKa = 4.48TIAQCGEE166 pKa = 3.91RR167 pKa = 11.84GFDD170 pKa = 3.69AVEE173 pKa = 3.82FDD175 pKa = 5.87NLDD178 pKa = 3.29SWSRR182 pKa = 11.84SSGALTLDD190 pKa = 4.03DD191 pKa = 5.23SIALATLLVDD201 pKa = 4.15KK202 pKa = 11.07AADD205 pKa = 3.47AGLAAGQKK213 pKa = 10.2NSAEE217 pKa = 4.01LATRR221 pKa = 11.84GRR223 pKa = 11.84DD224 pKa = 3.15EE225 pKa = 5.27AGFLFAIAEE234 pKa = 4.0EE235 pKa = 4.34CAAFDD240 pKa = 3.67EE241 pKa = 4.84CDD243 pKa = 3.66AYY245 pKa = 11.61SDD247 pKa = 4.06VYY249 pKa = 8.78GTNVIDD255 pKa = 4.47IEE257 pKa = 4.25YY258 pKa = 10.62LEE260 pKa = 4.19TKK262 pKa = 10.21YY263 pKa = 11.12ADD265 pKa = 3.59EE266 pKa = 4.38MDD268 pKa = 3.58VAFAEE273 pKa = 4.84VCADD277 pKa = 3.67PSSIPLTTLRR287 pKa = 11.84DD288 pKa = 3.77RR289 pKa = 11.84DD290 pKa = 4.19LVTPDD295 pKa = 3.1NPDD298 pKa = 3.29YY299 pKa = 11.72VFDD302 pKa = 3.95ACC304 pKa = 6.06

Molecular weight: 32.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2T0VA62|A0A2T0VA62_9MICO 4-amino-4-deoxychorismate lyase OS=Glaciihabitans tibetensis OX=1266600 GN=B0I08_108159 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.56LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3456

0

3456

1121382

29

2180

324.5

34.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.143 ± 0.059

0.484 ± 0.01

5.902 ± 0.04

5.428 ± 0.041

3.251 ± 0.025

8.67 ± 0.033

1.864 ± 0.021

4.809 ± 0.036

2.074 ± 0.03

10.139 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.768 ± 0.018

2.362 ± 0.022

5.337 ± 0.033

2.792 ± 0.02

6.559 ± 0.045

6.533 ± 0.033

6.516 ± 0.044

8.911 ± 0.039

1.4 ± 0.017

2.059 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski