Pseudomonas phage TC7

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 70 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3S6RNE8|A0A3S6RNE8_9CAUD Uncharacterized protein OS=Pseudomonas phage TC7 OX=2065330 GN=TC7_025 PE=4 SV=1
MM1 pKa = 6.84TTFTSIVTTNPDD13 pKa = 2.89FGGFEE18 pKa = 4.59FYY20 pKa = 11.21VEE22 pKa = 4.52AGQQFDD28 pKa = 3.19EE29 pKa = 4.51SAYY32 pKa = 9.0EE33 pKa = 3.7EE34 pKa = 4.78AYY36 pKa = 10.08GVSVPSSVVEE46 pKa = 4.01EE47 pKa = 4.35MNAKK51 pKa = 9.74AAQLKK56 pKa = 10.48DD57 pKa = 3.9GEE59 pKa = 4.43WLNVSHH65 pKa = 6.81EE66 pKa = 4.12AA67 pKa = 4.44

Molecular weight: 7.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3S6RNI2|A0A3S6RNI2_9CAUD Uncharacterized protein OS=Pseudomonas phage TC7 OX=2065330 GN=TC7_032 PE=4 SV=1
MM1 pKa = 7.84SDD3 pKa = 3.58LGILFPEE10 pKa = 4.86PEE12 pKa = 4.24TIYY15 pKa = 11.29VNGAPVIVRR24 pKa = 11.84HH25 pKa = 5.54VRR27 pKa = 11.84LADD30 pKa = 3.6FEE32 pKa = 4.9LFGDD36 pKa = 4.02IASDD40 pKa = 3.8LLKK43 pKa = 10.73VLSDD47 pKa = 3.54GTVPAILQFGKK58 pKa = 8.97TGSAKK63 pKa = 9.78LRR65 pKa = 11.84KK66 pKa = 9.09ILRR69 pKa = 11.84RR70 pKa = 11.84TTNLSRR76 pKa = 11.84WRR78 pKa = 11.84VYY80 pKa = 10.61RR81 pKa = 11.84LPVDD85 pKa = 3.38VAMQIVMQVIRR96 pKa = 11.84VNAAFFARR104 pKa = 11.84AQQAAVTTLATLVGQQQQ121 pKa = 2.94

Molecular weight: 13.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

70

0

70

12670

40

713

181.0

19.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.581 ± 0.524

1.35 ± 0.194

5.154 ± 0.206

6.29 ± 0.257

3.015 ± 0.174

7.932 ± 0.34

1.736 ± 0.181

4.23 ± 0.235

3.978 ± 0.325

8.39 ± 0.283

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.305 ± 0.185

2.952 ± 0.168

5.643 ± 0.273

4.475 ± 0.274

7.624 ± 0.391

5.517 ± 0.245

5.722 ± 0.334

6.843 ± 0.256

1.815 ± 0.158

2.447 ± 0.188
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski