Anaerococcus lactolyticus ATCC 51172

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Tissierellia; Tissierellales; Peptoniphilaceae; Anaerococcus; Anaerococcus lactolyticus

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2252 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C2BDB1|C2BDB1_9FIRM Radical SAM protein TIGR01212 family OS=Anaerococcus lactolyticus ATCC 51172 OX=525254 GN=HMPREF0072_0331 PE=4 SV=1
MM1 pKa = 7.56SYY3 pKa = 11.03YY4 pKa = 10.25EE5 pKa = 3.7VRR7 pKa = 11.84ANVNQLISEE16 pKa = 4.18NYY18 pKa = 7.77EE19 pKa = 4.08DD20 pKa = 4.18YY21 pKa = 11.09VKK23 pKa = 11.07SLISIEE29 pKa = 4.21KK30 pKa = 10.03NINNEE35 pKa = 3.77DD36 pKa = 4.15CLNSIYY42 pKa = 11.06NEE44 pKa = 4.52FINDD48 pKa = 3.18NNAASIFDD56 pKa = 4.21ADD58 pKa = 3.04IDD60 pKa = 4.42GYY62 pKa = 10.22IEE64 pKa = 4.13KK65 pKa = 10.62LGIDD69 pKa = 3.94LEE71 pKa = 4.46DD72 pKa = 3.35EE73 pKa = 4.27RR74 pKa = 11.84VIHH77 pKa = 6.32GEE79 pKa = 3.71PVSYY83 pKa = 10.81EE84 pKa = 3.91EE85 pKa = 4.01EE86 pKa = 4.0QEE88 pKa = 4.31RR89 pKa = 11.84EE90 pKa = 3.51VDD92 pKa = 3.25RR93 pKa = 11.84EE94 pKa = 4.03KK95 pKa = 11.11FNKK98 pKa = 9.52NDD100 pKa = 3.63YY101 pKa = 10.86EE102 pKa = 4.15NLKK105 pKa = 10.14PDD107 pKa = 4.29LDD109 pKa = 3.95NASFTLYY116 pKa = 10.73DD117 pKa = 3.53YY118 pKa = 11.67LLYY121 pKa = 10.9DD122 pKa = 3.53NQAYY126 pKa = 10.48LSDD129 pKa = 5.4AINEE133 pKa = 4.24VADD136 pKa = 3.64QSVDD140 pKa = 2.68IYY142 pKa = 11.6YY143 pKa = 10.66SDD145 pKa = 4.45LLKK148 pKa = 10.86SLPDD152 pKa = 3.8LEE154 pKa = 4.91NGGYY158 pKa = 7.84YY159 pKa = 9.86EE160 pKa = 4.11RR161 pKa = 11.84AAEE164 pKa = 4.08EE165 pKa = 4.08FGLTGDD171 pKa = 4.45LYY173 pKa = 11.27KK174 pKa = 10.92DD175 pKa = 3.07IMTAQYY181 pKa = 10.68LRR183 pKa = 11.84NIDD186 pKa = 3.82EE187 pKa = 4.95LYY189 pKa = 11.02EE190 pKa = 4.15NLDD193 pKa = 4.59DD194 pKa = 3.96IVKK197 pKa = 10.46SVAISIAEE205 pKa = 4.23DD206 pKa = 3.66YY207 pKa = 9.54EE208 pKa = 4.56LPLTEE213 pKa = 5.38GIEE216 pKa = 3.96EE217 pKa = 4.19ALNNISDD224 pKa = 4.36CNKK227 pKa = 9.33HH228 pKa = 6.85DD229 pKa = 4.73SIEE232 pKa = 4.34PIIDD236 pKa = 3.61EE237 pKa = 4.56LKK239 pKa = 10.29DD240 pKa = 3.57TIGYY244 pKa = 7.49EE245 pKa = 3.94AQDD248 pKa = 3.19IAKK251 pKa = 9.36EE252 pKa = 3.99KK253 pKa = 10.55LSRR256 pKa = 11.84EE257 pKa = 4.37GIDD260 pKa = 3.11INDD263 pKa = 4.89DD264 pKa = 3.13IEE266 pKa = 4.37EE267 pKa = 4.48YY268 pKa = 10.33IEE270 pKa = 4.69EE271 pKa = 4.09ITVYY275 pKa = 10.55EE276 pKa = 4.01LDD278 pKa = 3.96GNQNMMDD285 pKa = 5.15LINDD289 pKa = 3.97KK290 pKa = 10.73IEE292 pKa = 4.41EE293 pKa = 4.13IKK295 pKa = 10.76EE296 pKa = 3.72DD297 pKa = 3.87LEE299 pKa = 4.39INKK302 pKa = 10.06DD303 pKa = 3.4MSRR306 pKa = 3.42

Molecular weight: 35.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C2BE23|C2BE23_9FIRM Glucose/Sorbosone dehydrogenase (Fragment) OS=Anaerococcus lactolyticus ATCC 51172 OX=525254 GN=HMPREF0072_0593 PE=4 SV=1
LL1 pKa = 6.4QAGVRR6 pKa = 11.84PAVRR10 pKa = 11.84QPLRR14 pKa = 11.84LPPXGAGRR22 pKa = 11.84GHH24 pKa = 6.37LQGRR28 pKa = 11.84VQALSGNAGRR38 pKa = 11.84RR39 pKa = 11.84QAVLHH44 pKa = 6.42PGRR47 pKa = 11.84CGALRR52 pKa = 11.84TVRR55 pKa = 11.84GQHH58 pKa = 6.67LLGHH62 pKa = 6.07PWWRR66 pKa = 11.84AGAGVPGVRR75 pKa = 11.84RR76 pKa = 11.84LQAAA80 pKa = 3.74

Molecular weight: 8.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2252

0

2252

650064

29

5653

288.7

32.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.233 ± 0.055

0.755 ± 0.018

6.722 ± 0.047

7.55 ± 0.061

4.638 ± 0.043

6.264 ± 0.05

1.308 ± 0.021

9.154 ± 0.064

9.464 ± 0.071

9.32 ± 0.069

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.506 ± 0.033

5.653 ± 0.046

2.75 ± 0.035

2.347 ± 0.028

3.756 ± 0.04

6.131 ± 0.039

4.7 ± 0.051

5.925 ± 0.047

0.616 ± 0.014

4.209 ± 0.039
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski