Mycoplasma putrefaciens Mput9231

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Tenericutes; Mollicutes; Mycoplasmatales; Mycoplasmataceae; Mycoplasma; Mycoplasma putrefaciens

Average proteome isoelectric point is 7.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 666 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M9WGF1|M9WGF1_9MOLU YrdC-like domain-containing protein OS=Mycoplasma putrefaciens Mput9231 OX=1292033 GN=MPUT9231_0490 PE=4 SV=1
MM1 pKa = 7.27TNKK4 pKa = 9.61TNLNLVYY11 pKa = 10.38DD12 pKa = 4.18YY13 pKa = 11.23LHH15 pKa = 6.46EE16 pKa = 4.5VKK18 pKa = 10.59KK19 pKa = 9.04PTSLNQIWQAISGDD33 pKa = 3.89VISQKK38 pKa = 10.37KK39 pKa = 10.6DD40 pKa = 3.51EE41 pKa = 4.56ISVIADD47 pKa = 4.02LYY49 pKa = 11.36GDD51 pKa = 3.65MVLDD55 pKa = 3.49NRR57 pKa = 11.84FALTSDD63 pKa = 3.88SLWALSIDD71 pKa = 4.57PEE73 pKa = 4.25VEE75 pKa = 4.09DD76 pKa = 5.05IKK78 pKa = 11.26RR79 pKa = 11.84QFANNFVDD87 pKa = 4.1EE88 pKa = 4.5SEE90 pKa = 4.45KK91 pKa = 10.72KK92 pKa = 10.45SRR94 pKa = 11.84TSNSQDD100 pKa = 3.82DD101 pKa = 5.38LDD103 pKa = 4.49DD104 pKa = 3.31QTTILDD110 pKa = 3.49EE111 pKa = 5.03DD112 pKa = 4.85DD113 pKa = 4.45YY114 pKa = 11.81FNQEE118 pKa = 3.81LDD120 pKa = 3.37EE121 pKa = 6.57DD122 pKa = 4.64YY123 pKa = 11.5DD124 pKa = 4.84DD125 pKa = 5.26EE126 pKa = 6.61FYY128 pKa = 11.37DD129 pKa = 5.19EE130 pKa = 5.38NDD132 pKa = 4.09DD133 pKa = 3.92YY134 pKa = 12.09DD135 pKa = 6.26DD136 pKa = 5.62IEE138 pKa = 5.66DD139 pKa = 4.79SFNLDD144 pKa = 3.34HH145 pKa = 8.14DD146 pKa = 4.94DD147 pKa = 6.02DD148 pKa = 4.97YY149 pKa = 12.23DD150 pKa = 3.67EE151 pKa = 4.66

Molecular weight: 17.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M9WBL1|M9WBL1_9MOLU Hydrolase_4 domain-containing protein OS=Mycoplasma putrefaciens Mput9231 OX=1292033 GN=MPUT9231_0610 PE=3 SV=1
MM1 pKa = 7.32KK2 pKa = 9.47RR3 pKa = 11.84TWQPSKK9 pKa = 10.02IKK11 pKa = 9.82HH12 pKa = 5.45ARR14 pKa = 11.84VHH16 pKa = 5.94GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.44NGRR28 pKa = 11.84KK29 pKa = 9.39VINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.3GRR39 pKa = 11.84SKK41 pKa = 11.12LSAA44 pKa = 3.74

Molecular weight: 5.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

666

0

666

236353

37

1800

354.9

40.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.431 ± 0.086

0.569 ± 0.024

5.81 ± 0.073

5.899 ± 0.099

5.175 ± 0.087

4.514 ± 0.087

1.364 ± 0.033

9.504 ± 0.089

10.139 ± 0.093

9.816 ± 0.103

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.836 ± 0.041

7.306 ± 0.11

2.581 ± 0.063

4.492 ± 0.071

2.924 ± 0.056

6.569 ± 0.083

5.426 ± 0.054

5.814 ± 0.065

1.019 ± 0.038

3.81 ± 0.061
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski