Spiroplasma taiwanense CT-1

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Tenericutes; Mollicutes; Entomoplasmatales; Spiroplasmataceae; Spiroplasma; Spiroplasma taiwanense

Average proteome isoelectric point is 7.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 989 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S5LZW5|S5LZW5_9MOLU Uncharacterized protein OS=Spiroplasma taiwanense CT-1 OX=1276220 GN=STAIW_v1c06490 PE=4 SV=1
MM1 pKa = 7.67GIIWCDD7 pKa = 3.04EE8 pKa = 3.96SLDD11 pKa = 3.63MNFYY15 pKa = 11.19QNFINVNFLISGIWTDD31 pKa = 3.22RR32 pKa = 11.84YY33 pKa = 10.78SVVFEE38 pKa = 4.28KK39 pKa = 10.61ATVEE43 pKa = 4.02

Molecular weight: 5.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S5MDB6|S5MDB6_9MOLU Uncharacterized protein OS=Spiroplasma taiwanense CT-1 OX=1276220 GN=STAIW_v1c11150 PE=4 SV=1
MM1 pKa = 7.32KK2 pKa = 9.47RR3 pKa = 11.84TWQPSKK9 pKa = 10.03IKK11 pKa = 10.14HH12 pKa = 5.53ARR14 pKa = 11.84THH16 pKa = 5.77GFRR19 pKa = 11.84ARR21 pKa = 11.84MVTKK25 pKa = 10.46NGRR28 pKa = 11.84KK29 pKa = 8.88IIKK32 pKa = 9.57ARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.34GRR39 pKa = 11.84AKK41 pKa = 9.64LTAA44 pKa = 4.21

Molecular weight: 5.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

989

0

989

294780

29

1741

298.1

34.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.52 ± 0.077

0.672 ± 0.023

5.043 ± 0.056

7.322 ± 0.074

5.829 ± 0.081

4.795 ± 0.083

1.104 ± 0.028

10.92 ± 0.072

10.312 ± 0.095

9.623 ± 0.075

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.068 ± 0.033

8.077 ± 0.099

2.307 ± 0.043

3.033 ± 0.043

2.671 ± 0.052

6.705 ± 0.076

4.875 ± 0.055

5.206 ± 0.067

1.019 ± 0.026

3.898 ± 0.059
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski