Streptomyces roseochromogenus subsp. oscitans DS 12.976

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; Streptomyces roseochromogenus; Streptomyces roseochromogenus subsp. oscitans

Average proteome isoelectric point is 6.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8885 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|V6JQ06|V6JQ06_STRRC Prephenate dehydrogenase OS=Streptomyces roseochromogenus subsp. oscitans DS 12.976 OX=1352936 GN=M878_35595 PE=3 SV=1
MM1 pKa = 6.61QQEE4 pKa = 4.4APGGEE9 pKa = 4.07ALEE12 pKa = 4.15VWIDD16 pKa = 3.41QDD18 pKa = 3.99LCTGDD23 pKa = 5.01GICAQYY29 pKa = 10.9APEE32 pKa = 4.23VFEE35 pKa = 5.9LDD37 pKa = 3.05IDD39 pKa = 3.65GLAYY43 pKa = 10.73VKK45 pKa = 10.72GADD48 pKa = 3.75DD49 pKa = 4.74ALVQAVGATVPVPLPLLVDD68 pKa = 3.9VVDD71 pKa = 4.63SARR74 pKa = 11.84EE75 pKa = 3.94CPGEE79 pKa = 4.38CIHH82 pKa = 6.4VRR84 pKa = 11.84RR85 pKa = 11.84VSDD88 pKa = 3.27KK89 pKa = 10.85VEE91 pKa = 4.17VYY93 pKa = 10.9GPDD96 pKa = 3.36AEE98 pKa = 4.31

Molecular weight: 10.41 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|V6JPP1|V6JPP1_STRRC AB hydrolase-1 domain-containing protein OS=Streptomyces roseochromogenus subsp. oscitans DS 12.976 OX=1352936 GN=M878_43660 PE=4 SV=1
MM1 pKa = 7.58LPPLRR6 pKa = 11.84LLTVRR11 pKa = 11.84PGLRR15 pKa = 11.84ILLVPGLLVRR25 pKa = 11.84VLLLMTRR32 pKa = 11.84LLVTRR37 pKa = 11.84LLVSRR42 pKa = 11.84LLIRR46 pKa = 11.84IVLRR50 pKa = 11.84VALWVALRR58 pKa = 11.84VWRR61 pKa = 11.84APWRR65 pKa = 11.84LLIWPHH71 pKa = 5.02GQQ73 pKa = 3.0

Molecular weight: 8.57 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8885

0

8885

2650407

29

5130

298.3

32.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.459 ± 0.035

0.856 ± 0.008

5.909 ± 0.022

5.557 ± 0.029

2.744 ± 0.018

9.298 ± 0.028

2.449 ± 0.014

3.239 ± 0.018

2.214 ± 0.02

10.415 ± 0.032

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.74 ± 0.011

1.797 ± 0.014

6.056 ± 0.022

2.922 ± 0.015

7.984 ± 0.031

5.065 ± 0.024

6.254 ± 0.025

8.315 ± 0.024

1.545 ± 0.009

2.18 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski