Bifidobacterium reuteri DSM 23975

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium; Bifidobacterium reuteri

Average proteome isoelectric point is 5.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2149 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A087CSX4|A0A087CSX4_9BIFI Putative solute binding protein of ABC transporter system OS=Bifidobacterium reuteri DSM 23975 OX=1437610 GN=BREU_1553 PE=4 SV=1
MM1 pKa = 7.13AQDD4 pKa = 3.51YY5 pKa = 10.08DD6 pKa = 3.82SPRR9 pKa = 11.84NKK11 pKa = 10.84DD12 pKa = 3.11EE13 pKa = 5.7DD14 pKa = 4.16EE15 pKa = 4.93EE16 pKa = 4.45SLQALGKK23 pKa = 9.71SAQNSSSDD31 pKa = 3.5IDD33 pKa = 3.98DD34 pKa = 4.76DD35 pKa = 4.39EE36 pKa = 4.37NAIAEE41 pKa = 4.35DD42 pKa = 4.23YY43 pKa = 9.11EE44 pKa = 5.05LPGADD49 pKa = 4.92LSNEE53 pKa = 4.01DD54 pKa = 3.61SSVTVIPMQGDD65 pKa = 3.56EE66 pKa = 5.01FVCSQCFLVKK76 pKa = 10.28HH77 pKa = 6.4RR78 pKa = 11.84SQLAYY83 pKa = 9.38MDD85 pKa = 4.69EE86 pKa = 4.9DD87 pKa = 3.82GQPVCEE93 pKa = 4.29EE94 pKa = 4.37CASS97 pKa = 3.45

Molecular weight: 10.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A087CPE0|A0A087CPE0_9BIFI LytR_C domain-containing protein OS=Bifidobacterium reuteri DSM 23975 OX=1437610 GN=BREU_1960 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 9.6RR3 pKa = 11.84TFQPNNRR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84HH13 pKa = 4.82MKK15 pKa = 9.64HH16 pKa = 5.7GFRR19 pKa = 11.84LRR21 pKa = 11.84MRR23 pKa = 11.84TRR25 pKa = 11.84SGRR28 pKa = 11.84AVINRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.06GRR39 pKa = 11.84KK40 pKa = 8.87SLSAA44 pKa = 3.86

Molecular weight: 5.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2149

0

2149

805358

33

3267

374.8

40.84

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.223 ± 0.072

0.913 ± 0.016

6.66 ± 0.049

5.55 ± 0.044

3.413 ± 0.031

8.025 ± 0.048

2.187 ± 0.026

5.334 ± 0.04

3.798 ± 0.045

8.781 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.567 ± 0.025

3.38 ± 0.038

4.669 ± 0.036

3.457 ± 0.033

5.924 ± 0.06

6.133 ± 0.046

6.16 ± 0.042

7.655 ± 0.045

1.391 ± 0.024

2.782 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski