Siegesbeckia yellow vein Guangxi virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus

Average proteome isoelectric point is 7.81

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q0KFV8|Q0KFV8_9GEMI Transcriptional activator protein OS=Siegesbeckia yellow vein Guangxi virus OX=379728 GN=AC2 PE=3 SV=1
MM1 pKa = 7.87PLPKK5 pKa = 10.12KK6 pKa = 10.46FLINARR12 pKa = 11.84NYY14 pKa = 9.36FLTYY18 pKa = 7.93PHH20 pKa = 7.15CSLTKK25 pKa = 10.57EE26 pKa = 4.11EE27 pKa = 4.73ALSQILALSTPTNKK41 pKa = 10.09LFVRR45 pKa = 11.84ICRR48 pKa = 11.84EE49 pKa = 3.71LHH51 pKa = 6.47EE52 pKa = 6.12DD53 pKa = 3.78GTPHH57 pKa = 6.98LHH59 pKa = 7.02ILIQFEE65 pKa = 5.16GKK67 pKa = 10.08FKK69 pKa = 10.55CQNNRR74 pKa = 11.84FFDD77 pKa = 3.66LTSPNRR83 pKa = 11.84SAHH86 pKa = 5.14FHH88 pKa = 6.56PNIQGAKK95 pKa = 9.34SSSDD99 pKa = 2.92VKK101 pKa = 10.47TYY103 pKa = 8.75MEE105 pKa = 4.98KK106 pKa = 10.9DD107 pKa = 3.13GDD109 pKa = 3.89ILDD112 pKa = 4.09NGVFQVDD119 pKa = 3.31GRR121 pKa = 11.84SARR124 pKa = 11.84GGCQSANDD132 pKa = 4.26AYY134 pKa = 10.9AEE136 pKa = 4.72AINSGSKK143 pKa = 10.61ASALNILRR151 pKa = 11.84EE152 pKa = 4.05KK153 pKa = 10.62APKK156 pKa = 10.5DD157 pKa = 3.42FVLQFHH163 pKa = 6.68NLNSNLDD170 pKa = 4.14RR171 pKa = 11.84IFTPPMEE178 pKa = 4.56VYY180 pKa = 10.41VSPFISSSFDD190 pKa = 3.28QVPDD194 pKa = 3.66EE195 pKa = 4.24LEE197 pKa = 3.85EE198 pKa = 4.18WVSEE202 pKa = 4.4NIADD206 pKa = 3.77AAARR210 pKa = 11.84PWRR213 pKa = 11.84PVSIVIEE220 pKa = 4.4GDD222 pKa = 3.2SRR224 pKa = 11.84TGKK227 pKa = 8.52TMWARR232 pKa = 11.84SLGPHH237 pKa = 6.71NYY239 pKa = 10.18LCGHH243 pKa = 7.38LDD245 pKa = 4.18LSPKK249 pKa = 10.15VYY251 pKa = 11.07SNDD254 pKa = 2.25AWYY257 pKa = 10.92NVIDD261 pKa = 5.2DD262 pKa = 4.41VDD264 pKa = 3.61PHH266 pKa = 5.85YY267 pKa = 11.0LKK269 pKa = 10.7HH270 pKa = 6.2FKK272 pKa = 10.7EE273 pKa = 4.46FMGAQRR279 pKa = 11.84DD280 pKa = 3.81WQSNTKK286 pKa = 9.26YY287 pKa = 10.63GKK289 pKa = 9.49PIQIKK294 pKa = 10.42GGIPTIFLCNPGPTASYY311 pKa = 10.58IEE313 pKa = 4.21YY314 pKa = 10.49LDD316 pKa = 3.92EE317 pKa = 4.7EE318 pKa = 4.75KK319 pKa = 11.19NSALKK324 pKa = 10.45AWAIKK329 pKa = 10.11NATFITLNGPLYY341 pKa = 10.77SGTNQSPAQDD351 pKa = 3.74SQEE354 pKa = 4.11EE355 pKa = 4.22EE356 pKa = 4.4GDD358 pKa = 4.06QEE360 pKa = 4.58TEE362 pKa = 4.08DD363 pKa = 3.54

Molecular weight: 40.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q0KFW1|Q0KFW1_9GEMI Protein V2 OS=Siegesbeckia yellow vein Guangxi virus OX=379728 GN=AV2 PE=3 SV=1
MM1 pKa = 7.71AMRR4 pKa = 11.84PAAADD9 pKa = 3.38IVISTPASKK18 pKa = 10.29VRR20 pKa = 11.84RR21 pKa = 11.84RR22 pKa = 11.84LNFDD26 pKa = 3.11SPYY29 pKa = 9.74RR30 pKa = 11.84SSAAAPTVLVTNNRR44 pKa = 11.84SRR46 pKa = 11.84RR47 pKa = 11.84GWAQRR52 pKa = 11.84PMYY55 pKa = 9.58RR56 pKa = 11.84KK57 pKa = 8.84PRR59 pKa = 11.84IYY61 pKa = 10.89RR62 pKa = 11.84MFRR65 pKa = 11.84TPDD68 pKa = 3.15VPRR71 pKa = 11.84GCEE74 pKa = 4.34GPCKK78 pKa = 9.21VQSYY82 pKa = 7.99EE83 pKa = 3.88KK84 pKa = 10.76KK85 pKa = 10.36NDD87 pKa = 3.35VNHH90 pKa = 6.76TGTYY94 pKa = 10.17LCVSDD99 pKa = 3.55VTRR102 pKa = 11.84GNGLTHH108 pKa = 6.02RR109 pKa = 11.84TGKK112 pKa = 10.1RR113 pKa = 11.84FCIKK117 pKa = 10.31NIYY120 pKa = 10.02IMGKK124 pKa = 8.19IWMDD128 pKa = 3.38EE129 pKa = 4.01NIKK132 pKa = 9.32TKK134 pKa = 10.61NHH136 pKa = 6.02TNTVMFWLVRR146 pKa = 11.84DD147 pKa = 3.99RR148 pKa = 11.84RR149 pKa = 11.84PVTTPYY155 pKa = 11.3GFGEE159 pKa = 4.51AFNMYY164 pKa = 10.55DD165 pKa = 4.21NEE167 pKa = 4.43PSTATIKK174 pKa = 10.93NDD176 pKa = 3.08LRR178 pKa = 11.84DD179 pKa = 3.56RR180 pKa = 11.84LQVIRR185 pKa = 11.84RR186 pKa = 11.84FTSTVTGGQYY196 pKa = 10.8ASKK199 pKa = 9.98EE200 pKa = 3.71QAIVKK205 pKa = 9.7KK206 pKa = 10.25FYY208 pKa = 10.52KK209 pKa = 9.71IYY211 pKa = 10.32HH212 pKa = 5.9HH213 pKa = 5.92VTYY216 pKa = 10.89NHH218 pKa = 5.99QEE220 pKa = 3.62QAKK223 pKa = 9.66YY224 pKa = 10.54EE225 pKa = 4.12NHH227 pKa = 6.33TEE229 pKa = 3.99NALLLYY235 pKa = 8.57MASTHH240 pKa = 6.41ASNPVYY246 pKa = 9.86ATLKK250 pKa = 9.47IRR252 pKa = 11.84IYY254 pKa = 10.65FYY256 pKa = 11.33DD257 pKa = 3.43SVQNN261 pKa = 3.87

Molecular weight: 30.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1111

97

363

185.2

21.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.581 ± 0.795

2.07 ± 0.631

5.401 ± 0.628

4.59 ± 0.864

4.14 ± 0.554

4.59 ± 0.505

3.42 ± 0.361

6.121 ± 0.626

5.851 ± 0.429

7.111 ± 0.953

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.34 ± 0.474

5.671 ± 0.717

5.851 ± 0.58

5.581 ± 1.079

6.301 ± 1.14

7.741 ± 1.238

7.111 ± 1.055

5.041 ± 0.782

1.35 ± 0.2

4.14 ± 0.759
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski