Hubei sobemo-like virus 10

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KET5|A0A1L3KET5_9VIRU RNA-directed RNA polymerase OS=Hubei sobemo-like virus 10 OX=1923195 PE=4 SV=1
MM1 pKa = 7.11IAKK4 pKa = 9.58VLAFIAWCTKK14 pKa = 7.84GTNLLTLIMALYY26 pKa = 9.88IVNQWLQPVMASMADD41 pKa = 3.81RR42 pKa = 11.84IFEE45 pKa = 4.39VYY47 pKa = 10.21SGLRR51 pKa = 11.84LRR53 pKa = 11.84FNLFWDD59 pKa = 5.42GYY61 pKa = 10.57RR62 pKa = 11.84PGNPGWTFATNTHH75 pKa = 6.11RR76 pKa = 11.84LAYY79 pKa = 9.09QVHH82 pKa = 6.07HH83 pKa = 6.65AWANNMSPPEE93 pKa = 3.67ILGYY97 pKa = 9.17LVCAYY102 pKa = 9.87YY103 pKa = 10.33AAHH106 pKa = 6.09YY107 pKa = 10.21VVILCMFVRR116 pKa = 11.84KK117 pKa = 9.24QLAFLYY123 pKa = 10.66SRR125 pKa = 11.84IQSDD129 pKa = 3.81VLLDD133 pKa = 3.71FAEE136 pKa = 4.41KK137 pKa = 9.48MRR139 pKa = 11.84PGSMLEE145 pKa = 3.93PATNSPKK152 pKa = 10.26FQVEE156 pKa = 3.85VWVRR160 pKa = 11.84TDD162 pKa = 2.64KK163 pKa = 11.17TGFYY167 pKa = 10.54KK168 pKa = 10.52SGQAFCTKK176 pKa = 10.39FGFFTAYY183 pKa = 10.06HH184 pKa = 4.95VVEE187 pKa = 4.86DD188 pKa = 3.83AVEE191 pKa = 4.13VKK193 pKa = 10.37LVNRR197 pKa = 11.84VDD199 pKa = 3.46SQTYY203 pKa = 9.23EE204 pKa = 4.32LIIPASRR211 pKa = 11.84FQQVEE216 pKa = 4.07SDD218 pKa = 3.8VALLTVTSNEE228 pKa = 3.74IGVLQTPQCKK238 pKa = 10.07LMDD241 pKa = 4.95LGLMARR247 pKa = 11.84SGLFVRR253 pKa = 11.84VQAYY257 pKa = 9.67GSQTMGLLEE266 pKa = 4.19PSEE269 pKa = 4.84AFGLCSYY276 pKa = 10.36KK277 pKa = 10.83GSTIKK282 pKa = 10.72GFSGSPYY289 pKa = 8.18YY290 pKa = 10.64CGNNVFGMHH299 pKa = 6.67IGGSSEE305 pKa = 3.88NLGFEE310 pKa = 4.0AAYY313 pKa = 10.45LYY315 pKa = 10.32MLGKK319 pKa = 10.28RR320 pKa = 11.84NSEE323 pKa = 3.94DD324 pKa = 3.59TEE326 pKa = 5.2DD327 pKa = 3.88YY328 pKa = 10.91LLKK331 pKa = 10.54EE332 pKa = 4.34LEE334 pKa = 4.08RR335 pKa = 11.84DD336 pKa = 3.41EE337 pKa = 5.77DD338 pKa = 4.5FVWQRR343 pKa = 11.84SPYY346 pKa = 10.4DD347 pKa = 3.67PDD349 pKa = 3.26EE350 pKa = 4.65ARR352 pKa = 11.84VKK354 pKa = 10.99LGGKK358 pKa = 9.24YY359 pKa = 10.49YY360 pKa = 10.63NVDD363 pKa = 2.69MSTIKK368 pKa = 10.61KK369 pKa = 7.5MEE371 pKa = 3.83KK372 pKa = 9.17MKK374 pKa = 9.95TGRR377 pKa = 11.84RR378 pKa = 11.84APVYY382 pKa = 10.55DD383 pKa = 3.89PDD385 pKa = 4.01YY386 pKa = 10.79EE387 pKa = 4.27EE388 pKa = 5.23EE389 pKa = 4.41GLSEE393 pKa = 4.47ASDD396 pKa = 3.98AEE398 pKa = 4.63TEE400 pKa = 4.26DD401 pKa = 4.12SLPLAPRR408 pKa = 11.84GGLQYY413 pKa = 11.22DD414 pKa = 3.55DD415 pKa = 4.87SKK417 pKa = 12.03NLIAPQQSSAPAGVSGTPTTPQDD440 pKa = 3.74TVPQSALKK448 pKa = 10.01ALNGITLEE456 pKa = 4.06SRR458 pKa = 11.84KK459 pKa = 9.9RR460 pKa = 11.84LAKK463 pKa = 10.45SILDD467 pKa = 3.67GRR469 pKa = 11.84EE470 pKa = 3.76LTPALLRR477 pKa = 11.84DD478 pKa = 3.92LSISTSVSSKK488 pKa = 9.33SKK490 pKa = 10.03RR491 pKa = 11.84RR492 pKa = 11.84SAYY495 pKa = 9.4RR496 pKa = 11.84QKK498 pKa = 11.13KK499 pKa = 9.19KK500 pKa = 10.67NN501 pKa = 3.37

Molecular weight: 56.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KET5|A0A1L3KET5_9VIRU RNA-directed RNA polymerase OS=Hubei sobemo-like virus 10 OX=1923195 PE=4 SV=1
MM1 pKa = 7.72AMTKK5 pKa = 10.63DD6 pKa = 3.15MSRR9 pKa = 11.84YY10 pKa = 7.26WRR12 pKa = 11.84EE13 pKa = 3.92EE14 pKa = 3.19FDD16 pKa = 4.03KK17 pKa = 11.5AMLEE21 pKa = 3.8IDD23 pKa = 3.84MDD25 pKa = 4.43SSPGLCQFAQYY36 pKa = 10.9GPTNAAVFKK45 pKa = 10.69YY46 pKa = 10.42KK47 pKa = 10.78DD48 pKa = 3.79GVWCKK53 pKa = 10.66DD54 pKa = 3.06RR55 pKa = 11.84EE56 pKa = 4.27KK57 pKa = 10.74EE58 pKa = 4.03VRR60 pKa = 11.84DD61 pKa = 3.52VVFFRR66 pKa = 11.84MQQLLRR72 pKa = 11.84GEE74 pKa = 4.52LVADD78 pKa = 4.88DD79 pKa = 3.88IKK81 pKa = 11.04MFVKK85 pKa = 10.54PEE87 pKa = 3.68PHH89 pKa = 6.74KK90 pKa = 10.2PEE92 pKa = 4.42KK93 pKa = 9.29MAQGRR98 pKa = 11.84FRR100 pKa = 11.84LISAVSLVDD109 pKa = 3.6ALVDD113 pKa = 4.72RR114 pKa = 11.84ILFGWLSRR122 pKa = 11.84KK123 pKa = 9.86ALTVVGKK130 pKa = 7.43TPCLTGWSPVHH141 pKa = 6.42GGWKK145 pKa = 9.47YY146 pKa = 9.87IEE148 pKa = 4.51HH149 pKa = 7.0RR150 pKa = 11.84FSGKK154 pKa = 9.53PVVCLDD160 pKa = 3.27KK161 pKa = 11.4SSWDD165 pKa = 3.18WTVQEE170 pKa = 4.34WLITAWFEE178 pKa = 4.11FLKK181 pKa = 11.03GLALGADD188 pKa = 4.01SWWVEE193 pKa = 3.76LVSARR198 pKa = 11.84FQMLFEE204 pKa = 4.19RR205 pKa = 11.84PIYY208 pKa = 10.3QFADD212 pKa = 3.73GTRR215 pKa = 11.84VQQKK219 pKa = 9.36YY220 pKa = 8.91VGIMKK225 pKa = 10.13SGCFLTLILNSVGQSMLHH243 pKa = 5.15YY244 pKa = 9.85VASLRR249 pKa = 11.84IGRR252 pKa = 11.84NPIRR256 pKa = 11.84NQPISVGDD264 pKa = 3.72DD265 pKa = 3.23TTQEE269 pKa = 4.16SFPEE273 pKa = 4.0LEE275 pKa = 4.26EE276 pKa = 4.32YY277 pKa = 10.49INQISRR283 pKa = 11.84LGAKK287 pKa = 9.68VKK289 pKa = 10.25GFKK292 pKa = 9.26VRR294 pKa = 11.84HH295 pKa = 4.27WVEE298 pKa = 4.49FIGFAWIKK306 pKa = 8.94GVCVPAYY313 pKa = 7.02WQKK316 pKa = 11.13HH317 pKa = 4.39LFKK320 pKa = 10.58LQYY323 pKa = 10.6SHH325 pKa = 8.05LEE327 pKa = 4.17DD328 pKa = 3.7VLQSYY333 pKa = 10.11QILYY337 pKa = 10.56ANEE340 pKa = 3.67PVMFEE345 pKa = 4.02LLRR348 pKa = 11.84RR349 pKa = 11.84LAKK352 pKa = 9.85EE353 pKa = 3.97VNPEE357 pKa = 3.85LVMSSYY363 pKa = 9.59EE364 pKa = 3.78AKK366 pKa = 10.74AIMNYY371 pKa = 10.32

Molecular weight: 43.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

872

371

501

436.0

49.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.11 ± 0.573

1.491 ± 0.08

4.931 ± 0.05

6.078 ± 0.246

4.817 ± 0.531

6.651 ± 0.284

1.491 ± 0.249

4.128 ± 0.455

6.307 ± 0.441

9.862 ± 0.269

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.44 ± 0.21

2.982 ± 0.519

4.472 ± 0.27

4.472 ± 0.239

5.505 ± 0.098

7.339 ± 0.717

4.472 ± 0.948

7.454 ± 0.737

2.408 ± 0.859

4.587 ± 0.512
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski