Daeseongdong virus 1

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 6.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0S2RRE2|A0A0S2RRE2_9VIRU DiSB-ORF2_chro domain-containing protein OS=Daeseongdong virus 1 OX=1758881 PE=4 SV=1
MM1 pKa = 7.69KK2 pKa = 10.59SFLLFVLFLTTSVHH16 pKa = 5.58AAVFIKK22 pKa = 10.67DD23 pKa = 3.28IVRR26 pKa = 11.84QLTATRR32 pKa = 11.84EE33 pKa = 3.84KK34 pKa = 10.62HH35 pKa = 5.96LLYY38 pKa = 10.86SKK40 pKa = 10.82LQMRR44 pKa = 11.84LHH46 pKa = 6.32FVGEE50 pKa = 4.31FDD52 pKa = 3.05ISPYY56 pKa = 10.82KK57 pKa = 10.2FDD59 pKa = 4.33PEE61 pKa = 4.28CLSVYY66 pKa = 8.85RR67 pKa = 11.84TNDD70 pKa = 2.56WFFSKK75 pKa = 11.0CEE77 pKa = 4.01LPSNCSKK84 pKa = 10.69PLVDD88 pKa = 4.86VIDD91 pKa = 4.5RR92 pKa = 11.84NWLGQEE98 pKa = 3.97TVLCHH103 pKa = 5.85GVHH106 pKa = 6.84PSEE109 pKa = 4.47TSDD112 pKa = 2.96RR113 pKa = 11.84MNFFSMEE120 pKa = 4.1FVPSNFTFGRR130 pKa = 11.84PITIDD135 pKa = 2.84GRR137 pKa = 11.84EE138 pKa = 3.94FTRR141 pKa = 11.84FEE143 pKa = 4.4DD144 pKa = 4.38FVPVSFFEE152 pKa = 3.8WFYY155 pKa = 10.39MVKK158 pKa = 10.38SPGDD162 pKa = 3.4SYY164 pKa = 11.85GIVPKK169 pKa = 10.43FCSEE173 pKa = 4.35TYY175 pKa = 10.12RR176 pKa = 11.84DD177 pKa = 4.11STTLPPNVNVKK188 pKa = 10.45SDD190 pKa = 3.47GNTLCLTEE198 pKa = 3.93IQYY201 pKa = 10.4DD202 pKa = 3.81QSDD205 pKa = 4.86CRR207 pKa = 11.84PKK209 pKa = 10.08WYY211 pKa = 9.25PSYY214 pKa = 11.01HH215 pKa = 6.83SIFADD220 pKa = 2.6IDD222 pKa = 4.01FPVEE226 pKa = 4.02FSGVPYY232 pKa = 10.86SPGSYY237 pKa = 9.8SATNNKK243 pKa = 9.41VIGADD248 pKa = 3.2VYY250 pKa = 11.21MKK252 pKa = 10.81DD253 pKa = 3.52NLGNTPTHH261 pKa = 6.51VIDD264 pKa = 4.17KK265 pKa = 10.93VGGAYY270 pKa = 8.72MVTYY274 pKa = 10.37SPYY277 pKa = 10.37NGLGPIYY284 pKa = 9.68IVKK287 pKa = 8.59ATSVIPKK294 pKa = 9.74GDD296 pKa = 3.48SRR298 pKa = 11.84CFEE301 pKa = 4.11LQSRR305 pKa = 11.84YY306 pKa = 9.69HH307 pKa = 7.19SPFEE311 pKa = 3.63ILIAKK316 pKa = 9.47ISKK319 pKa = 9.67FFRR322 pKa = 11.84QEE324 pKa = 3.48LEE326 pKa = 3.94YY327 pKa = 11.21LLEE330 pKa = 3.9FLIAFAEE337 pKa = 4.71KK338 pKa = 9.99IASILIAILGEE349 pKa = 3.78IMNVVLSLVPYY360 pKa = 10.6GDD362 pKa = 4.29LFYY365 pKa = 10.91TSAFLAAVTYY375 pKa = 10.53LFTRR379 pKa = 11.84DD380 pKa = 3.35VILALVPNIAIYY392 pKa = 10.03LVRR395 pKa = 11.84IYY397 pKa = 10.62LKK399 pKa = 10.81SAII402 pKa = 4.18

Molecular weight: 45.89 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0S2RRD3|A0A0S2RRD3_9VIRU Non-structural protein 3 OS=Daeseongdong virus 1 OX=1758881 PE=4 SV=1
MM1 pKa = 7.14SANRR5 pKa = 11.84ASTKK9 pKa = 10.06SVSRR13 pKa = 11.84RR14 pKa = 11.84PVAPRR19 pKa = 11.84QVVITTRR26 pKa = 11.84NAPRR30 pKa = 11.84AQKK33 pKa = 10.34SPKK36 pKa = 9.74KK37 pKa = 8.56PTPAKK42 pKa = 10.26EE43 pKa = 3.96AGFDD47 pKa = 3.85LNKK50 pKa = 9.98VVSDD54 pKa = 3.7ISSTFLAGLNKK65 pKa = 9.96PLVLLTLVLVVALVFTHH82 pKa = 6.6VNDD85 pKa = 4.82FSSGVLGKK93 pKa = 9.21WVAEE97 pKa = 4.01KK98 pKa = 10.69SANNSLALWVHH109 pKa = 4.98QNQMKK114 pKa = 10.3FLGLAIFAPAVLDD127 pKa = 3.86SPDD130 pKa = 4.18KK131 pKa = 10.87IRR133 pKa = 11.84LALALACVFWVMLVPQASVYY153 pKa = 10.23EE154 pKa = 4.09YY155 pKa = 10.46AIQAIALHH163 pKa = 6.07TYY165 pKa = 9.98FRR167 pKa = 11.84VRR169 pKa = 11.84LQNSRR174 pKa = 11.84VFILLLVAALYY185 pKa = 9.91FFGHH189 pKa = 6.19ITLSGTPGGTPVTNSSIGGG208 pKa = 3.59

Molecular weight: 22.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

2971

208

2361

990.3

112.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.89 ± 1.347

2.02 ± 0.406

6.193 ± 1.121

5.419 ± 1.113

6.429 ± 0.569

4.645 ± 0.182

2.423 ± 0.24

5.453 ± 0.563

5.587 ± 0.26

8.852 ± 0.967

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.221 ± 0.25

4.948 ± 0.448

4.746 ± 0.599

2.726 ± 0.315

5.756 ± 0.768

8.078 ± 0.317

6.16 ± 0.219

8.112 ± 0.908

0.606 ± 0.304

3.736 ± 0.657
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski