Methanosphaera sp. rholeuAM6

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Methanomada group; Methanobacteria; Methanobacteriales; Methanobacteriaceae; Methanosphaera; unclassified Methanosphaera

Average proteome isoelectric point is 5.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1750 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A328RXT7|A0A328RXT7_9EURY Uncharacterized protein (Fragment) OS=Methanosphaera sp. rholeuAM6 OX=1945580 GN=BZ135_08995 PE=4 SV=1
MM1 pKa = 7.63LLLIVFTITITLSSVNAGLFDD22 pKa = 5.68LDD24 pKa = 4.42STNDD28 pKa = 4.2DD29 pKa = 3.57INVTDD34 pKa = 5.19LEE36 pKa = 4.46INYY39 pKa = 9.27EE40 pKa = 4.25GYY42 pKa = 8.12STYY45 pKa = 10.81EE46 pKa = 4.25VNCKK50 pKa = 8.27LTPKK54 pKa = 10.41KK55 pKa = 10.81DD56 pKa = 3.44FDD58 pKa = 4.04YY59 pKa = 11.82LEE61 pKa = 4.36MFVIFYY67 pKa = 10.77DD68 pKa = 3.74SDD70 pKa = 3.96DD71 pKa = 3.83AVLDD75 pKa = 3.63KK76 pKa = 10.95STLVWNTNQPTKK88 pKa = 10.85DD89 pKa = 3.32QVIKK93 pKa = 11.07VSGTAYY99 pKa = 9.78VQNDD103 pKa = 3.11NEE105 pKa = 4.34KK106 pKa = 9.12PVRR109 pKa = 11.84AEE111 pKa = 3.96VYY113 pKa = 8.82FTDD116 pKa = 4.62GLDD119 pKa = 3.97AEE121 pKa = 4.85PEE123 pKa = 4.08SAIYY127 pKa = 10.15SEE129 pKa = 4.28NVTMGG134 pKa = 3.03

Molecular weight:
15.11 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A328RY80|A0A328RY80_9EURY Uncharacterized protein (Fragment) OS=Methanosphaera sp. rholeuAM6 OX=1945580 GN=BZ135_03755 PE=4 SV=1
MM1 pKa = 7.43SRR3 pKa = 11.84NKK5 pKa = 10.09YY6 pKa = 9.7LPKK9 pKa = 10.37KK10 pKa = 10.31LRR12 pKa = 11.84LAKK15 pKa = 10.19KK16 pKa = 8.58NKK18 pKa = 7.27QNRR21 pKa = 11.84RR22 pKa = 11.84VPIFAMMKK30 pKa = 8.74TSRR33 pKa = 11.84KK34 pKa = 9.59LRR36 pKa = 11.84THH38 pKa = 6.39PKK40 pKa = 7.85ARR42 pKa = 11.84QWRR45 pKa = 11.84RR46 pKa = 11.84SKK48 pKa = 10.85IKK50 pKa = 10.31VV51 pKa = 3.11

Molecular weight:
6.3 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1750

0

1750

513664

25

3340

293.5

33.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.383 ± 0.072

1.156 ± 0.026

6.311 ± 0.051

7.302 ± 0.092

3.797 ± 0.045

5.96 ± 0.072

1.653 ± 0.028

9.491 ± 0.067

7.65 ± 0.085

8.484 ± 0.078

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.325 ± 0.033

6.989 ± 0.115

3.033 ± 0.036

2.698 ± 0.041

3.199 ± 0.046

6.293 ± 0.058

6.6 ± 0.125

6.704 ± 0.067

0.583 ± 0.018

4.371 ± 0.059

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski