Enterococcus phage vB_EfaS_Ef6.4

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Efquatrovirus; unclassified Efquatrovirus

Average proteome isoelectric point is 5.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 69 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6DSJ5|A0A4D6DSJ5_9CAUD Uncharacterized protein OS=Enterococcus phage vB_EfaS_Ef6.4 OX=2546632 PE=4 SV=1
MM1 pKa = 6.95KK2 pKa = 10.48QEE4 pKa = 4.29AKK6 pKa = 10.5FSRR9 pKa = 11.84KK10 pKa = 9.13VVDD13 pKa = 3.37YY14 pKa = 11.16LEE16 pKa = 4.87AKK18 pKa = 9.66GAVVNVNTATIYY30 pKa = 10.95DD31 pKa = 3.67RR32 pKa = 11.84VGRR35 pKa = 11.84ADD37 pKa = 4.48IEE39 pKa = 4.17ACYY42 pKa = 9.91LGYY45 pKa = 10.9YY46 pKa = 9.05IALEE50 pKa = 4.33LKK52 pKa = 9.0TGNYY56 pKa = 9.45QPDD59 pKa = 4.1PLQITYY65 pKa = 9.41LQQVRR70 pKa = 11.84CAGGYY75 pKa = 10.4GLLLRR80 pKa = 11.84DD81 pKa = 4.03TLDD84 pKa = 3.91DD85 pKa = 5.3LEE87 pKa = 6.23DD88 pKa = 4.2LLLHH92 pKa = 7.0LDD94 pKa = 3.47QMDD97 pKa = 3.85NGVEE101 pKa = 4.1YY102 pKa = 9.84PFTYY106 pKa = 9.69EE107 pKa = 5.08QPDD110 pKa = 3.86LPDD113 pKa = 3.59IADD116 pKa = 4.24DD117 pKa = 4.8KK118 pKa = 11.82LEE120 pKa = 3.88VWYY123 pKa = 9.78DD124 pKa = 3.27

Molecular weight: 14.22 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6DSG5|A0A4D6DSG5_9CAUD Glutaredoxin-like protein OS=Enterococcus phage vB_EfaS_Ef6.4 OX=2546632 PE=4 SV=1
MM1 pKa = 7.84PAPKK5 pKa = 9.78PIMVKK10 pKa = 10.03DD11 pKa = 3.87LRR13 pKa = 11.84TQQTRR18 pKa = 11.84AFKK21 pKa = 10.84SQTEE25 pKa = 3.63ADD27 pKa = 3.22KK28 pKa = 11.06FYY30 pKa = 10.82GKK32 pKa = 10.46KK33 pKa = 9.78SGYY36 pKa = 10.29FKK38 pKa = 10.64DD39 pKa = 3.37IKK41 pKa = 10.07TKK43 pKa = 10.48LGGKK47 pKa = 8.05NRR49 pKa = 11.84HH50 pKa = 4.89YY51 pKa = 10.72EE52 pKa = 3.99IIEE55 pKa = 4.19VVV57 pKa = 3.03

Molecular weight: 6.62 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

69

0

69

12559

51

1409

182.0

20.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.765 ± 0.476

0.605 ± 0.114

6.219 ± 0.246

8.177 ± 0.498

4.101 ± 0.231

6.465 ± 0.563

1.433 ± 0.182

6.704 ± 0.225

9.149 ± 0.383

8.241 ± 0.313

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.787 ± 0.172

6.37 ± 0.261

2.882 ± 0.213

3.615 ± 0.199

3.273 ± 0.196

5.12 ± 0.283

6.665 ± 0.402

6.848 ± 0.243

1.298 ± 0.115

4.284 ± 0.361
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski