Methylobacterium symbioticum

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Methylobacteriaceae; Methylobacterium

Average proteome isoelectric point is 6.96

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5664 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A509E7Z2|A0A509E7Z2_9RHIZ Putative glucarate transporter OS=Methylobacterium symbioticum OX=2584084 GN=gudP_1 PE=4 SV=1
MM1 pKa = 7.6VDD3 pKa = 3.31LTGYY7 pKa = 10.93RR8 pKa = 11.84LTFDD12 pKa = 5.35DD13 pKa = 5.19EE14 pKa = 4.8FNQLSVSATGSGTVWASTRR33 pKa = 11.84PGSILRR39 pKa = 11.84PGVDD43 pKa = 2.43IGFGQSAFVDD53 pKa = 4.47PSTGVQPFQIVNGALQIQAAPATGAVADD81 pKa = 4.29LVYY84 pKa = 10.61PGLWTSGLLQASNSFSQTYY103 pKa = 10.29GYY105 pKa = 11.13FEE107 pKa = 4.19MRR109 pKa = 11.84AEE111 pKa = 4.33LPAEE115 pKa = 4.16TGAWPGFWLLNADD128 pKa = 3.95GVWPPEE134 pKa = 3.59IDD136 pKa = 3.08IVEE139 pKa = 4.56AYY141 pKa = 10.24GADD144 pKa = 3.47PTALTNTIHH153 pKa = 6.19TGEE156 pKa = 4.34TGQPTHH162 pKa = 4.83QTIWSNQPTLSAGYY176 pKa = 8.44HH177 pKa = 4.96TFGALWTASTITFFYY192 pKa = 10.62DD193 pKa = 3.64GNPVGQVATPSDD205 pKa = 3.91MHH207 pKa = 8.0GPMYY211 pKa = 10.39PLVNLAITRR220 pKa = 11.84GIEE223 pKa = 4.38GITDD227 pKa = 3.62SVKK230 pKa = 10.13TMSVDD235 pKa = 3.47YY236 pKa = 10.71IRR238 pKa = 11.84VYY240 pKa = 10.87SADD243 pKa = 3.53PSATAVALQTVSSPDD258 pKa = 3.43GADD261 pKa = 2.83TGGLYY266 pKa = 10.37GASASNVTPPPPTAKK281 pKa = 10.06PDD283 pKa = 3.48TLTIRR288 pKa = 11.84VSGDD292 pKa = 2.65SWNGAPKK299 pKa = 10.2FVVSVDD305 pKa = 3.59GTPLGQVFSVDD316 pKa = 3.2ADD318 pKa = 3.65HH319 pKa = 7.62AAGQWQDD326 pKa = 3.07VTVQGNFGAGPHH338 pKa = 5.05QVAVTYY344 pKa = 10.82INDD347 pKa = 3.7GNEE350 pKa = 3.74NPYY353 pKa = 10.79YY354 pKa = 10.53DD355 pKa = 3.62AAANKK360 pKa = 9.33VFNDD364 pKa = 3.05RR365 pKa = 11.84NLFVTGIALNGTSYY379 pKa = 11.83GLGSVVSNSASVGWDD394 pKa = 3.22LLDD397 pKa = 4.19PNAAVMAANGTLIFGTAGGTVTPIDD422 pKa = 4.43PPTTPALPTTPATPTTPTAPAAGQDD447 pKa = 3.39TLTIRR452 pKa = 11.84ISGDD456 pKa = 2.77SWNGAPQFVVSVDD469 pKa = 3.46GTQVAGPFDD478 pKa = 3.79VTADD482 pKa = 3.47HH483 pKa = 7.46AAGQWQDD490 pKa = 3.09VSVQGDD496 pKa = 4.34FGAGPHH502 pKa = 5.35QVTITYY508 pKa = 10.67LNDD511 pKa = 3.41GNEE514 pKa = 4.1NPYY517 pKa = 10.71YY518 pKa = 10.53DD519 pKa = 3.94AVANKK524 pKa = 9.45VFNDD528 pKa = 3.13RR529 pKa = 11.84NLFVTGIALNGTAYY543 pKa = 10.55GLDD546 pKa = 3.03AVVSNAAAAGWDD558 pKa = 3.5RR559 pKa = 11.84LDD561 pKa = 3.85PNAAVMAASGTLVFGTGAATPGTSSPTTPLPTTPTAPVTAPGQDD605 pKa = 3.04TLTIHH610 pKa = 7.33ISGDD614 pKa = 3.15SWNGAPQFVVSVDD627 pKa = 3.44GTSVGGPFDD636 pKa = 3.46VTADD640 pKa = 3.47HH641 pKa = 7.46AAGQWQDD648 pKa = 3.09VSVQGDD654 pKa = 4.34FGAGPHH660 pKa = 5.35QVTITYY666 pKa = 10.67LNDD669 pKa = 3.41GNEE672 pKa = 4.1NPYY675 pKa = 10.71YY676 pKa = 10.53DD677 pKa = 3.94AVANKK682 pKa = 9.45VFNDD686 pKa = 3.13RR687 pKa = 11.84NLFVTGIDD695 pKa = 3.99LNGTAYY701 pKa = 10.53GLDD704 pKa = 2.97AVVSNMAAAGWDD716 pKa = 3.44RR717 pKa = 11.84LDD719 pKa = 3.85PNAAVMAASGTLVFGTGAA737 pKa = 3.44

Molecular weight: 76.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A509EDV8|A0A509EDV8_9RHIZ Lead cadmium zinc and mercury-transporting ATPase OS=Methylobacterium symbioticum OX=2584084 GN=zntA_3 PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 9.15RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATAGGRR28 pKa = 11.84KK29 pKa = 9.12VIARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AHH37 pKa = 4.95GRR39 pKa = 11.84KK40 pKa = 9.25RR41 pKa = 11.84LSAA44 pKa = 4.01

Molecular weight: 5.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5664

0

5664

1690294

29

3678

298.4

32.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.447 ± 0.059

0.845 ± 0.011

5.403 ± 0.024

5.516 ± 0.03

3.329 ± 0.021

9.209 ± 0.043

1.944 ± 0.014

4.349 ± 0.025

2.415 ± 0.03

10.621 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.044 ± 0.016

2.051 ± 0.019

5.847 ± 0.029

2.852 ± 0.021

8.285 ± 0.042

4.81 ± 0.025

5.213 ± 0.032

7.584 ± 0.027

1.219 ± 0.014

2.018 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski