Shigella phage SfII (Shigella flexneri bacteriophage II) (Bacteriophage SfII)

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 7.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 58 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S5FM63|S5FM63_BPSF2 Probable tape measure protein OS=Shigella phage SfII OX=66284 GN=SfII_16 PE=3 SV=1
MM1 pKa = 7.69KK2 pKa = 8.86LTPVIAALRR11 pKa = 11.84ARR13 pKa = 11.84CPYY16 pKa = 10.06FEE18 pKa = 4.39NRR20 pKa = 11.84VAGAAQFKK28 pKa = 10.19NLPEE32 pKa = 4.11VGKK35 pKa = 10.51LRR37 pKa = 11.84LPAAYY42 pKa = 9.22VVPGDD47 pKa = 4.84DD48 pKa = 4.34SPGEE52 pKa = 4.13NKK54 pKa = 10.35SQTDD58 pKa = 3.3YY59 pKa = 10.55WQEE62 pKa = 3.75LKK64 pKa = 10.91EE65 pKa = 4.23GFSVVVILSNGRR77 pKa = 11.84DD78 pKa = 3.43EE79 pKa = 5.21RR80 pKa = 11.84GQFASYY86 pKa = 11.14DD87 pKa = 3.57VVDD90 pKa = 5.36DD91 pKa = 3.79VRR93 pKa = 11.84QMLFKK98 pKa = 10.84ALLGWNPEE106 pKa = 3.8ACGNPITYY114 pKa = 10.35DD115 pKa = 3.18GGTLLDD121 pKa = 4.22LNRR124 pKa = 11.84HH125 pKa = 4.61EE126 pKa = 6.12LIYY129 pKa = 10.88QFDD132 pKa = 3.96FSVISEE138 pKa = 4.21LTEE141 pKa = 4.82DD142 pKa = 4.41DD143 pKa = 3.88TRR145 pKa = 11.84QQDD148 pKa = 3.89DD149 pKa = 4.49LNSLDD154 pKa = 4.01EE155 pKa = 4.63LRR157 pKa = 11.84TLAIDD162 pKa = 4.15VDD164 pKa = 4.29YY165 pKa = 10.82LDD167 pKa = 5.29PGNGPDD173 pKa = 4.82GDD175 pKa = 4.3IEE177 pKa = 4.36HH178 pKa = 6.63HH179 pKa = 5.92TEE181 pKa = 3.68IPLPSS186 pKa = 3.38

Molecular weight: 20.79 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S5FM74|S5FM74_BPSF2 Excisionase OS=Shigella phage SfII OX=66284 GN=xis PE=4 SV=1
MM1 pKa = 7.9PARR4 pKa = 11.84KK5 pKa = 9.27VCQTFFRR12 pKa = 11.84NALASTHH19 pKa = 5.94QYY21 pKa = 8.87RR22 pKa = 11.84QNAIIDD28 pKa = 3.71SAAALVGGASLSLTSIGRR46 pKa = 11.84HH47 pKa = 4.94LPGPARR53 pKa = 11.84VKK55 pKa = 10.94DD56 pKa = 3.56KK57 pKa = 10.77IKK59 pKa = 10.81RR60 pKa = 11.84VDD62 pKa = 3.25RR63 pKa = 11.84LLGNKK68 pKa = 9.15RR69 pKa = 11.84LHH71 pKa = 6.51NDD73 pKa = 2.37IPLIFKK79 pKa = 10.59NITSMMTNKK88 pKa = 10.3LSWCVIAVDD97 pKa = 3.42WSGYY101 pKa = 9.02PFQEE105 pKa = 4.02YY106 pKa = 9.92HH107 pKa = 5.94VLRR110 pKa = 11.84ASLLCDD116 pKa = 3.31GRR118 pKa = 11.84SIPLMSQVFPSKK130 pKa = 10.69KK131 pKa = 9.45NNEE134 pKa = 4.0AVEE137 pKa = 4.15IAFLDD142 pKa = 4.01ALSGAISPRR151 pKa = 11.84TRR153 pKa = 11.84VVIVTDD159 pKa = 4.57AGFQSAWFRR168 pKa = 11.84HH169 pKa = 5.7IKK171 pKa = 8.66SQGWDD176 pKa = 3.72FIGRR180 pKa = 11.84IRR182 pKa = 11.84GVVKK186 pKa = 10.52FRR188 pKa = 11.84LDD190 pKa = 3.24SDD192 pKa = 3.48KK193 pKa = 11.59DD194 pKa = 3.04KK195 pKa = 11.08WLDD198 pKa = 3.08IKK200 pKa = 10.03MCRR203 pKa = 11.84GSSEE207 pKa = 4.03AKK209 pKa = 9.91YY210 pKa = 11.05LEE212 pKa = 4.52TGTLARR218 pKa = 11.84KK219 pKa = 9.34KK220 pKa = 9.96RR221 pKa = 11.84SQCEE225 pKa = 3.68GHH227 pKa = 6.37FYY229 pKa = 10.7LYY231 pKa = 10.48KK232 pKa = 10.52HH233 pKa = 5.87SPKK236 pKa = 10.08GRR238 pKa = 11.84KK239 pKa = 7.75SRR241 pKa = 11.84RR242 pKa = 11.84ARR244 pKa = 11.84GRR246 pKa = 11.84PGLPTTEE253 pKa = 4.73KK254 pKa = 9.27EE255 pKa = 3.8QKK257 pKa = 10.11AAGRR261 pKa = 11.84EE262 pKa = 3.69PWLIFSNTAEE272 pKa = 4.16FNAKK276 pKa = 10.04KK277 pKa = 10.2IMKK280 pKa = 9.46LYY282 pKa = 9.4SRR284 pKa = 11.84RR285 pKa = 11.84MQIEE289 pKa = 3.85QNFRR293 pKa = 11.84DD294 pKa = 3.9EE295 pKa = 3.93KK296 pKa = 10.64SEE298 pKa = 3.97RR299 pKa = 11.84FGFGLRR305 pKa = 11.84ASRR308 pKa = 11.84SRR310 pKa = 11.84RR311 pKa = 11.84GEE313 pKa = 3.82RR314 pKa = 11.84FLVLSLLVTLASIVLWLLGYY334 pKa = 10.4HH335 pKa = 6.12FEE337 pKa = 4.76NKK339 pKa = 9.56GFHH342 pKa = 6.7LKK344 pKa = 10.04YY345 pKa = 9.97QANSLKK351 pKa = 10.15KK352 pKa = 9.98RR353 pKa = 11.84RR354 pKa = 11.84VLSFLTLAEE363 pKa = 3.95NVLRR367 pKa = 11.84FNPEE371 pKa = 3.79LLRR374 pKa = 11.84RR375 pKa = 11.84AQPEE379 pKa = 4.21KK380 pKa = 10.34ILCRR384 pKa = 11.84LASTYY389 pKa = 10.96RR390 pKa = 11.84SMVLAYY396 pKa = 10.23

Molecular weight: 45.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

58

0

58

12616

56

611

217.5

24.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.743 ± 0.354

1.3 ± 0.167

5.485 ± 0.273

5.588 ± 0.296

4.011 ± 0.294

6.983 ± 0.331

1.839 ± 0.149

5.929 ± 0.538

5.652 ± 0.325

9.028 ± 0.273

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.663 ± 0.172

4.328 ± 0.248

4.574 ± 0.205

3.686 ± 0.199

6.294 ± 0.43

6.476 ± 0.256

5.77 ± 0.305

6.714 ± 0.262

1.807 ± 0.138

3.131 ± 0.219
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski