Virgibacillus sp. SK37

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Virgibacillus; unclassified Virgibacillus

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3815 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A075JKX8|A0A075JKX8_9BACI Uncharacterized protein OS=Virgibacillus sp. SK37 OX=403957 GN=X953_04705 PE=4 SV=1
MM1 pKa = 7.07ITNEE5 pKa = 4.65QLVQCKK11 pKa = 9.64KK12 pKa = 10.89ALVDD16 pKa = 3.87RR17 pKa = 11.84QNEE20 pKa = 4.54LINQVQDD27 pKa = 3.45HH28 pKa = 5.91FEE30 pKa = 4.09KK31 pKa = 10.41TLEE34 pKa = 4.07LTKK37 pKa = 10.8EE38 pKa = 4.41SVGEE42 pKa = 3.58LSNYY46 pKa = 9.81DD47 pKa = 3.47NHH49 pKa = 6.62PADD52 pKa = 4.29MGTEE56 pKa = 4.08LYY58 pKa = 10.48EE59 pKa = 4.19RR60 pKa = 11.84EE61 pKa = 4.33KK62 pKa = 10.83DD63 pKa = 3.34TALNEE68 pKa = 4.11HH69 pKa = 7.03AEE71 pKa = 4.36TEE73 pKa = 4.19LEE75 pKa = 4.26EE76 pKa = 4.65INAALHH82 pKa = 6.34AIEE85 pKa = 5.02DD86 pKa = 3.77ATYY89 pKa = 10.06GICATCGADD98 pKa = 3.4IPFEE102 pKa = 4.02RR103 pKa = 11.84LQAVPTADD111 pKa = 3.02KK112 pKa = 10.9CIEE115 pKa = 4.07HH116 pKa = 6.99ASNNIFEE123 pKa = 4.63SPRR126 pKa = 11.84TVEE129 pKa = 4.24EE130 pKa = 4.25EE131 pKa = 4.21VFSPNINPNEE141 pKa = 4.1VTNEE145 pKa = 3.87EE146 pKa = 4.16QVGYY150 pKa = 10.05DD151 pKa = 5.88AEE153 pKa = 5.21DD154 pKa = 2.99SWQEE158 pKa = 3.56VSQYY162 pKa = 8.6GTSEE166 pKa = 4.32TPSDD170 pKa = 4.05FYY172 pKa = 11.75GNQDD176 pKa = 4.8DD177 pKa = 4.58YY178 pKa = 12.1DD179 pKa = 4.61DD180 pKa = 4.48MYY182 pKa = 11.36PNNEE186 pKa = 4.09EE187 pKa = 3.66NTGAVEE193 pKa = 3.97DD194 pKa = 4.64LEE196 pKa = 5.08NFLSSDD202 pKa = 2.85IDD204 pKa = 3.79GKK206 pKa = 9.92FTGVTPNHH214 pKa = 6.13NKK216 pKa = 10.1YY217 pKa = 9.28EE218 pKa = 4.1DD219 pKa = 4.08EE220 pKa = 4.48YY221 pKa = 10.47EE222 pKa = 4.1SEE224 pKa = 4.52YY225 pKa = 11.65

Molecular weight: 25.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A075JJ29|A0A075JJ29_9BACI Lipoprotein OS=Virgibacillus sp. SK37 OX=403957 GN=X953_00090 PE=3 SV=1
MM1 pKa = 7.59AKK3 pKa = 10.28KK4 pKa = 10.89SMIAKK9 pKa = 9.7QKK11 pKa = 10.44RR12 pKa = 11.84PQKK15 pKa = 8.76YY16 pKa = 6.84QVRR19 pKa = 11.84EE20 pKa = 3.73YY21 pKa = 10.01TRR23 pKa = 11.84CEE25 pKa = 3.77RR26 pKa = 11.84CGRR29 pKa = 11.84PHH31 pKa = 7.11SVIRR35 pKa = 11.84KK36 pKa = 8.62FKK38 pKa = 10.19LCRR41 pKa = 11.84ICFRR45 pKa = 11.84EE46 pKa = 3.99LAYY49 pKa = 9.84KK50 pKa = 10.19GQIPGVKK57 pKa = 9.25KK58 pKa = 10.9ASWW61 pKa = 3.03

Molecular weight: 7.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3815

0

3815

1046293

26

2585

274.3

30.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.792 ± 0.038

0.652 ± 0.012

5.232 ± 0.035

7.732 ± 0.054

4.497 ± 0.037

6.758 ± 0.037

2.062 ± 0.022

8.076 ± 0.042

7.201 ± 0.042

9.554 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.845 ± 0.02

4.706 ± 0.03

3.501 ± 0.024

3.781 ± 0.029

3.829 ± 0.031

5.882 ± 0.028

5.477 ± 0.025

6.796 ± 0.03

1.016 ± 0.016

3.611 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski