Gordonia phage Bowser

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Bowservirus; Gordonia virus Bowser

Average proteome isoelectric point is 6.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 67 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A160DF60|A0A160DF60_9CAUD Tyrosine integrase OS=Gordonia phage Bowser OX=1838063 GN=39 PE=3 SV=1
MM1 pKa = 7.88AILFEE6 pKa = 4.66DD7 pKa = 3.68WCTADD12 pKa = 4.86GGTDD16 pKa = 3.34LAGVVTLTAIGQQAAGALTLATVPQPIAVTAGAFSEE52 pKa = 4.89TVAPGDD58 pKa = 3.54YY59 pKa = 9.66MVTVEE64 pKa = 4.46LTAGADD70 pKa = 3.46EE71 pKa = 4.71VLASTDD77 pKa = 3.05TAAQRR82 pKa = 11.84IAVADD87 pKa = 4.01LDD89 pKa = 4.1TPQRR93 pKa = 11.84LRR95 pKa = 11.84DD96 pKa = 3.99LIGLGGPGQSPLAKK110 pKa = 10.32LNEE113 pKa = 4.46LVTAWIEE120 pKa = 4.15GVADD124 pKa = 3.38ATYY127 pKa = 11.12APVFPDD133 pKa = 3.23TGITYY138 pKa = 10.24DD139 pKa = 3.47GSGNVQTVTEE149 pKa = 4.22NGVTTTYY156 pKa = 10.29TYY158 pKa = 11.45NPDD161 pKa = 2.94GTVATDD167 pKa = 3.13TRR169 pKa = 11.84DD170 pKa = 3.37GVTRR174 pKa = 11.84TYY176 pKa = 11.03GYY178 pKa = 10.7DD179 pKa = 3.3GNGNLTSITVEE190 pKa = 4.16DD191 pKa = 3.72

Molecular weight: 19.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A160DCS8|A0A160DCS8_9CAUD Uncharacterized protein OS=Gordonia phage Bowser OX=1838063 GN=54 PE=4 SV=1
MM1 pKa = 7.35LTTIRR6 pKa = 11.84ARR8 pKa = 11.84LTALLIAAVAATTLSACDD26 pKa = 3.22SDD28 pKa = 5.98GYY30 pKa = 9.22TEE32 pKa = 4.1PVSNEE37 pKa = 3.72VCVDD41 pKa = 3.2RR42 pKa = 11.84SDD44 pKa = 5.57RR45 pKa = 11.84RR46 pKa = 11.84AQDD49 pKa = 3.57HH50 pKa = 5.83YY51 pKa = 11.34CEE53 pKa = 4.72SGLPNYY59 pKa = 9.08HH60 pKa = 5.15WWYY63 pKa = 10.29LPAGTYY69 pKa = 10.33VGIGQHH75 pKa = 4.81VTGGTNLRR83 pKa = 11.84PNRR86 pKa = 11.84VRR88 pKa = 11.84RR89 pKa = 11.84APTVTVTRR97 pKa = 11.84NATPRR102 pKa = 11.84TTATTPRR109 pKa = 11.84KK110 pKa = 7.57PTTKK114 pKa = 9.57ATPNKK119 pKa = 8.37TRR121 pKa = 11.84SRR123 pKa = 11.84STRR126 pKa = 3.13

Molecular weight: 13.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

67

0

67

14596

50

1704

217.9

23.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.579 ± 0.589

0.829 ± 0.162

6.714 ± 0.367

5.721 ± 0.372

2.693 ± 0.214

8.687 ± 0.449

1.939 ± 0.141

4.405 ± 0.202

2.795 ± 0.198

8.235 ± 0.285

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.179 ± 0.14

2.754 ± 0.185

6.084 ± 0.371

3.323 ± 0.175

7.434 ± 0.379

5.474 ± 0.208

6.666 ± 0.307

7.303 ± 0.266

2.124 ± 0.132

2.062 ± 0.181
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski