Streptomyces phage Scap1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Scapunavirus; Streptomyces virus Scap1

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 56 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2D1GP11|A0A2D1GP11_9CAUD Uncharacterized protein OS=Streptomyces phage Scap1 OX=2041354 GN=SEA_SCAP1_49 PE=4 SV=1
MM1 pKa = 7.74SVLQWDD7 pKa = 3.67QTGEE11 pKa = 3.93KK12 pKa = 9.99VYY14 pKa = 11.03EE15 pKa = 4.09NGVEE19 pKa = 3.85KK20 pKa = 10.84GVFYY24 pKa = 10.08TVSGAGVYY32 pKa = 10.77DD33 pKa = 5.27NGVAWNGLVSVTEE46 pKa = 4.32SPSGAEE52 pKa = 3.98VNKK55 pKa = 10.37QYY57 pKa = 11.51ADD59 pKa = 3.8NIVYY63 pKa = 10.62ASLRR67 pKa = 11.84SAEE70 pKa = 4.23EE71 pKa = 3.62FGATIEE77 pKa = 4.21AFTYY81 pKa = 9.9PLEE84 pKa = 4.94AIPALDD90 pKa = 4.23GSASPTPGLALGQQGRR106 pKa = 11.84PTFGFSYY113 pKa = 8.46VTKK116 pKa = 10.57VGNDD120 pKa = 3.59LNPDD124 pKa = 3.49AGEE127 pKa = 4.71KK128 pKa = 9.53IHH130 pKa = 6.59LVYY133 pKa = 10.5GATANPSEE141 pKa = 4.31KK142 pKa = 10.49AYY144 pKa = 7.99TTVNDD149 pKa = 3.83SPEE152 pKa = 3.78AATFSWEE159 pKa = 4.15LTTSPVQVGTIGGTAYY175 pKa = 9.89KK176 pKa = 9.49PLSTITVDD184 pKa = 3.5TTKK187 pKa = 11.06EE188 pKa = 3.58DD189 pKa = 3.55ADD191 pKa = 3.71AVNTLRR197 pKa = 11.84EE198 pKa = 4.18FLYY201 pKa = 8.99GTEE204 pKa = 4.27GTDD207 pKa = 3.5PSLPSPAAVVALFSGAVLTATPTEE231 pKa = 4.1PTYY234 pKa = 10.77DD235 pKa = 3.04TTTNVMTVPTITGVQYY251 pKa = 11.63YY252 pKa = 9.44MDD254 pKa = 5.27GEE256 pKa = 4.33LLAPGPQTAFTEE268 pKa = 4.22NHH270 pKa = 4.98VVEE273 pKa = 4.92ARR275 pKa = 11.84PAMGYY280 pKa = 10.67KK281 pKa = 8.97FTQPTDD287 pKa = 3.78SDD289 pKa = 3.62WLIGDD294 pKa = 4.35FF295 pKa = 4.98

Molecular weight: 31.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2D1GNV0|A0A2D1GNV0_9CAUD Uncharacterized protein OS=Streptomyces phage Scap1 OX=2041354 GN=SEA_SCAP1_48 PE=4 SV=1
MM1 pKa = 7.43RR2 pKa = 11.84TRR4 pKa = 11.84TRR6 pKa = 11.84RR7 pKa = 11.84PKK9 pKa = 10.46TEE11 pKa = 3.76FTDD14 pKa = 4.01LPRR17 pKa = 11.84SLRR20 pKa = 11.84SPSHH24 pKa = 5.49PTQVLWVFLFRR35 pKa = 11.84IDD37 pKa = 3.43TRR39 pKa = 11.84TSLMGNSLKK48 pKa = 10.68FAGFLGRR55 pKa = 11.84VFLVNTAAGVGAATGIIGTAVLAAKK80 pKa = 9.48FAKK83 pKa = 9.83PQLKK87 pKa = 9.89KK88 pKa = 10.53VWDD91 pKa = 5.16AIPVPTFEE99 pKa = 6.02DD100 pKa = 3.32EE101 pKa = 4.68KK102 pKa = 11.32EE103 pKa = 4.24NNTKK107 pKa = 10.64

Molecular weight: 11.9 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

56

0

56

13713

37

1748

244.9

27.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.582 ± 0.739

0.423 ± 0.085

6.359 ± 0.353

6.592 ± 0.43

3.77 ± 0.26

7.744 ± 0.494

1.626 ± 0.212

5.389 ± 0.245

6.118 ± 0.359

8.153 ± 0.525

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.625 ± 0.144

4.113 ± 0.179

4.193 ± 0.321

3.26 ± 0.177

5.214 ± 0.462

5.761 ± 0.293

6.782 ± 0.321

7.285 ± 0.225

1.51 ± 0.188

3.5 ± 0.356
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski