Proteome-pI 2.0 - Proteome Isoelectric Point Database

Clostridium botulinum (strain Hall / ATCC 3502 / NCTC 13319 / Type A)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; Clostridium botulinum; Clostridium botulinum A

Average proteome isoelectric point is 6.89

Get precalculated fractions of proteins

Acidic

pI < 6.8

6.8-7.4

pI > 7.4

Basic

All

Virtual 2D-PAGE plot for 3590 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions

Protein with the lowest isoelectric point:

>sp|A5I634|RS21_CLOBH 30S ribosomal protein S21 OS=Clostridium botulinum (strain Hall / ATCC 3502 / NCTC 13319 / Type A) OX=441771 GN=rpsU PE=3 SV=1

MM1 pKa = 7.56FIDD4 pKa = 5.06DD5 pKa = 4.43YY6 pKa = 11.44PYY8 pKa = 11.31LEE10 pKa = 4.82NNQNDD15 pKa = 3.9EE16 pKa = 4.36NMNMRR21 pKa = 11.84PMPYY25 pKa = 8.61MAWGGSPMYY34 pKa = 10.06NPYY37 pKa = 9.11MPNVDD42 pKa = 3.86CSMDD46 pKa = 3.98NINYY50 pKa = 10.43DD51 pKa = 4.25NMDD54 pKa = 3.53RR55 pKa = 11.84CNNNITNEE63 pKa = 4.06NEE65 pKa = 3.62DD66 pKa = 3.85YY67 pKa = 10.78IYY69 pKa = 10.4IDD71 pKa = 3.32NRR73 pKa = 11.84LPMTSDD79 pKa = 3.67PPQTTPTPTPTPTPTPAPAPTPTPAPAPTTQFLPSVQDD117 pKa = 4.3DD118 pKa = 3.2ILYY121 pKa = 9.36NQGYY125 pKa = 9.93LKK127 pKa = 9.12TQIGKK132 pKa = 9.16RR133 pKa = 11.84VKK135 pKa = 10.04IEE137 pKa = 3.8FLIGTNMLIDD147 pKa = 3.7RR148 pKa = 11.84EE149 pKa = 4.44GTLVGVGISYY159 pKa = 10.38VLINEE164 pKa = 3.9VDD166 pKa = 3.72TNDD169 pKa = 5.39LIMADD174 pKa = 3.5MYY176 pKa = 10.91SIKK179 pKa = 10.14FVRR182 pKa = 11.84IFDD185 pKa = 3.65

Molecular weight: 21.05 kDa
Isoelectric point according different methods:

Protein with the highest isoelectric point:

>tr|A5HYU9|A5HYU9_CLOBH Putative transcriptional regulator OS=Clostridium botulinum (strain Hall / ATCC 3502 / NCTC 13319 / Type A) OX=441771 GN=CBO0405 PE=4 SV=1

MM1 pKa = 7.59NNRR4 pKa = 11.84FLSGIAAGAILGATAGMLIVPQLDD28 pKa = 2.92RR29 pKa = 11.84GTRR32 pKa = 11.84RR33 pKa = 11.84RR34 pKa = 11.84IKK36 pKa = 9.09RR37 pKa = 11.84TSRR40 pKa = 11.84TMMGMAGNMMGNMRR54 pKa = 11.84GIMRR58 pKa = 4.92

Molecular weight: 6.31 kDa
Isoelectric point according different methods:

Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try ESI

ChTry ESI

ArgC ESI

LysN ESI

TryLysC ESI

Try MALDI

ChTry MALDI

ArgC MALDI

LysN MALDI

TryLysC MALDI

Try LTQ

ChTry LTQ

ArgC LTQ

LysN LTQ

TryLysC LTQ

Try MSlow

ChTry MSlow

ArgC MSlow

LysN MSlow

TryLysC MSlow

Try MShigh

ChTry MShigh

ArgC MShigh

LysN MShigh

TryLysC MShigh

General Statistics

Number of major isoforms	Number of additional isoforms	Number of all proteins	Number of amino acids	Min. Seq. Length	Max. Seq. Length	Avg. Seq. Length	Avg. Mol. Weight
3589	1	3590	1052452	23	2882	293.2	33.19

Amino acid frequency

Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu
5.424 ± 0.045	1.239 ± 0.016	5.338 ± 0.034	7.473 ± 0.052	4.375 ± 0.029	6.278 ± 0.038	1.359 ± 0.015	10.398 ± 0.053	9.905 ± 0.041	8.987 ± 0.041

Met	Asn	Gln	Pro	Arg	Ser	Thr	Val	Trp	Tyr
2.661 ± 0.021	6.565 ± 0.048	2.797 ± 0.022	2.189 ± 0.021	3.158 ± 0.03	6.134 ± 0.037	4.679 ± 0.025	6.193 ± 0.036	0.684 ± 0.013	4.162 ± 0.032

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here

See this proteome in:

Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski