Hafnia alvei FB1

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Hafniaceae; Hafnia; Hafnia alvei

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4059 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A097QZ12|A0A097QZ12_HAFAL Thiol:disulfide interchange protein OS=Hafnia alvei FB1 OX=1453496 GN=AT03_04455 PE=3 SV=1
MM1 pKa = 7.42SNVPTEE7 pKa = 4.39LKK9 pKa = 9.44YY10 pKa = 9.08TASHH14 pKa = 5.27EE15 pKa = 4.27WVRR18 pKa = 11.84AEE20 pKa = 4.06GEE22 pKa = 4.31GVYY25 pKa = 10.56SVGITEE31 pKa = 4.64HH32 pKa = 6.12AQEE35 pKa = 4.67LLGDD39 pKa = 3.92MVFIDD44 pKa = 4.45LPEE47 pKa = 4.45VGTSFDD53 pKa = 4.38AGDD56 pKa = 3.95DD57 pKa = 3.85CAVAEE62 pKa = 4.4SVKK65 pKa = 10.54AASDD69 pKa = 3.51IYY71 pKa = 11.37APISGEE77 pKa = 3.42IVAINDD83 pKa = 3.61EE84 pKa = 4.58LEE86 pKa = 4.39GAPEE90 pKa = 4.35LVNSAPYY97 pKa = 10.13SEE99 pKa = 4.54GWLFRR104 pKa = 11.84IKK106 pKa = 10.72ASDD109 pKa = 3.4EE110 pKa = 3.92AQIAGLLDD118 pKa = 3.46AAAYY122 pKa = 8.16EE123 pKa = 4.52SSIEE127 pKa = 4.11DD128 pKa = 3.19

Molecular weight: 13.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A097QXK7|A0A097QXK7_HAFAL HTH-type transcriptional repressor FabR OS=Hafnia alvei FB1 OX=1453496 GN=fabR PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.6RR12 pKa = 11.84NRR14 pKa = 11.84SHH16 pKa = 7.16GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.35NGRR28 pKa = 11.84LVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.36GRR39 pKa = 11.84TRR41 pKa = 11.84LTVSKK46 pKa = 10.95

Molecular weight: 5.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4059

0

4059

1290908

29

3857

318.0

35.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.421 ± 0.046

1.113 ± 0.013

5.182 ± 0.032

5.626 ± 0.04

3.885 ± 0.026

7.326 ± 0.038

2.298 ± 0.02

6.074 ± 0.039

4.418 ± 0.033

10.798 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.857 ± 0.02

3.913 ± 0.03

4.353 ± 0.027

4.66 ± 0.031

5.284 ± 0.034

6.216 ± 0.032

5.282 ± 0.034

7.023 ± 0.034

1.379 ± 0.018

2.892 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski