Bosea sp. Leaf344

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Boseaceae; Bosea; unclassified Bosea

Average proteome isoelectric point is 6.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3876 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0Q6KMY9|A0A0Q6KMY9_9BRAD 1-deoxy-D-xylulose-5-phosphate synthase OS=Bosea sp. Leaf344 OX=1736346 GN=dxs PE=3 SV=1
MM1 pKa = 7.18NVGGFSEE8 pKa = 5.17SIWHH12 pKa = 5.33FAGYY16 pKa = 10.15LRR18 pKa = 11.84LIEE21 pKa = 4.14PAARR25 pKa = 11.84PSDD28 pKa = 4.41LFDD31 pKa = 5.24GDD33 pKa = 4.49PLPPPGDD40 pKa = 3.77EE41 pKa = 3.78IYY43 pKa = 11.25GRR45 pKa = 11.84LSEE48 pKa = 4.6TLPPAQFEE56 pKa = 4.41EE57 pKa = 4.75TASQRR62 pKa = 11.84VLFVEE67 pKa = 5.27APAASPALPGLKK79 pKa = 9.8LASPDD84 pKa = 3.79FKK86 pKa = 10.85PISLNPSSFPALPRR100 pKa = 11.84RR101 pKa = 11.84DD102 pKa = 4.13APLQEE107 pKa = 4.38PDD109 pKa = 2.98IALEE113 pKa = 3.98GGIQFRR119 pKa = 11.84FLSQSRR125 pKa = 11.84SISVDD130 pKa = 3.34YY131 pKa = 11.04QPGGTEE137 pKa = 3.6TLLQLRR143 pKa = 11.84QINTMDD149 pKa = 3.95DD150 pKa = 3.5RR151 pKa = 11.84DD152 pKa = 4.34VILSDD157 pKa = 4.0ALPGVLPPEE166 pKa = 4.61IEE168 pKa = 3.89TGAAVAEE175 pKa = 4.33MLQQAQEE182 pKa = 4.13ATPDD186 pKa = 3.67SLPFEE191 pKa = 5.57AIGSTQALIAAITARR206 pKa = 11.84DD207 pKa = 3.7AAWAQSGQSPHH218 pKa = 7.19ADD220 pKa = 3.15PDD222 pKa = 3.7YY223 pKa = 10.21TPPSGLIVDD232 pKa = 4.6GVVVAAAPTGPTIEE246 pKa = 4.99QIAPWRR252 pKa = 11.84EE253 pKa = 3.61AASAPTQFVSQTVSEE268 pKa = 4.26AASTLGIGAVAEE280 pKa = 4.35TGLNQQINQAVILDD294 pKa = 3.94LNEE297 pKa = 4.45AVGSMIVGGDD307 pKa = 3.15AFFSRR312 pKa = 11.84GIVQVNILTDD322 pKa = 3.55SDD324 pKa = 4.05HH325 pKa = 8.05VDD327 pKa = 3.07IAIEE331 pKa = 4.18GAGSVGVFTTDD342 pKa = 3.13NQLHH346 pKa = 6.54NIAEE350 pKa = 4.49FVTHH354 pKa = 7.46DD355 pKa = 3.08MTARR359 pKa = 11.84YY360 pKa = 9.24SGAAATPYY368 pKa = 9.49WHH370 pKa = 7.27VDD372 pKa = 3.11TLAGNFYY379 pKa = 10.6DD380 pKa = 4.91VKK382 pKa = 10.54TLVQFNGLEE391 pKa = 4.35DD392 pKa = 3.86GDD394 pKa = 3.87RR395 pKa = 11.84TVQASEE401 pKa = 3.84GAYY404 pKa = 10.36FEE406 pKa = 5.6AKK408 pKa = 9.51TGLNGQVNYY417 pKa = 11.45AMVTSLDD424 pKa = 3.4QYY426 pKa = 11.33DD427 pKa = 4.05IIIIAGDD434 pKa = 3.3YY435 pKa = 10.6HH436 pKa = 6.17RR437 pKa = 11.84ADD439 pKa = 3.93WIFQYY444 pKa = 11.34NIMLDD449 pKa = 3.39SDD451 pKa = 4.07VAKK454 pKa = 10.58LYY456 pKa = 10.78AAGGTAEE463 pKa = 5.39DD464 pKa = 4.59DD465 pKa = 3.99VAVTTGFNSLTNSASITTYY484 pKa = 11.21DD485 pKa = 3.26SAAFAALNAAQHH497 pKa = 6.65DD498 pKa = 4.75LLSALAQGATVLTPHH513 pKa = 7.29ADD515 pKa = 2.69WGLNGNISGEE525 pKa = 4.09LKK527 pKa = 10.15ILYY530 pKa = 10.18VSGDD534 pKa = 3.8YY535 pKa = 11.27YY536 pKa = 11.28DD537 pKa = 4.3VNVITQINLMVDD549 pKa = 3.52ADD551 pKa = 3.82QAIQASAIGGEE562 pKa = 4.26LGVAAGGNSAANYY575 pKa = 10.66AEE577 pKa = 4.81IIDD580 pKa = 4.79PGTLSTSRR588 pKa = 11.84YY589 pKa = 8.96IGGEE593 pKa = 4.0AYY595 pKa = 10.44DD596 pKa = 4.44DD597 pKa = 5.19AILVQTNLVTDD608 pKa = 4.54DD609 pKa = 4.06DD610 pKa = 4.68TVTIHH615 pKa = 7.14DD616 pKa = 4.08TTTLVPEE623 pKa = 4.75FVAFAEE629 pKa = 4.27QTEE632 pKa = 4.72MPTEE636 pKa = 4.27DD637 pKa = 4.96APPLCKK643 pKa = 9.83PIEE646 pKa = 4.28ATPQDD651 pKa = 3.49ILII654 pKa = 4.57

Molecular weight: 69.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0Q6KBN6|A0A0Q6KBN6_9BRAD GCN5 family acetyltransferase OS=Bosea sp. Leaf344 OX=1736346 GN=ASG72_07820 PE=4 SV=1
MM1 pKa = 7.55SISGSLVPVAVGAVAVVLLLGLINMLRR28 pKa = 11.84GGSPGTSQRR37 pKa = 11.84LMRR40 pKa = 11.84LRR42 pKa = 11.84VILQFIAILVILGVLWWRR60 pKa = 11.84SGG62 pKa = 3.16

Molecular weight: 6.57 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3876

0

3876

1240282

41

3073

320.0

34.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.91 ± 0.054

0.82 ± 0.011

5.252 ± 0.035

5.577 ± 0.036

3.586 ± 0.023

9.018 ± 0.041

1.907 ± 0.018

5.057 ± 0.031

2.854 ± 0.031

10.777 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.355 ± 0.018

2.135 ± 0.024

5.486 ± 0.034

3.159 ± 0.019

7.581 ± 0.041

5.111 ± 0.029

4.932 ± 0.027

7.214 ± 0.035

1.273 ± 0.018

1.996 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski