Bacillus phage phi4B1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 56 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0M4R397|A0A0M4R397_9CAUD ArpU family transcriptional regulator OS=Bacillus phage phi4B1 OX=1643324 GN=XO26_0055 PE=4 SV=1
MM1 pKa = 7.26NVYY4 pKa = 10.12ILIRR8 pKa = 11.84EE9 pKa = 4.33TYY11 pKa = 9.27SYY13 pKa = 11.26CGDD16 pKa = 3.58FEE18 pKa = 5.05VISEE22 pKa = 4.16VNIEE26 pKa = 4.16GVFSKK31 pKa = 10.85EE32 pKa = 3.52LDD34 pKa = 3.4AKK36 pKa = 10.87LALLNSKK43 pKa = 8.4GTGYY47 pKa = 10.71DD48 pKa = 2.71AFYY51 pKa = 10.43IEE53 pKa = 4.73EE54 pKa = 4.22MEE56 pKa = 5.41LIQQ59 pKa = 5.63

Molecular weight: 6.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0M4R382|A0A0M4R382_9CAUD Uncharacterized protein OS=Bacillus phage phi4B1 OX=1643324 GN=XO26_0026 PE=4 SV=1
MM1 pKa = 7.61KK2 pKa = 10.35SPYY5 pKa = 10.31DD6 pKa = 3.59FYY8 pKa = 10.64ITPEE12 pKa = 4.03EE13 pKa = 4.16YY14 pKa = 10.41EE15 pKa = 3.67IAARR19 pKa = 11.84NGINRR24 pKa = 11.84RR25 pKa = 11.84TLNVRR30 pKa = 11.84VRR32 pKa = 11.84NLGWDD37 pKa = 3.39KK38 pKa = 10.97DD39 pKa = 3.57IAMTKK44 pKa = 10.19PLGSKK49 pKa = 10.49NSTGWRR55 pKa = 11.84KK56 pKa = 9.76VKK58 pKa = 10.53EE59 pKa = 3.82IALKK63 pKa = 10.87NGISRR68 pKa = 11.84QTYY71 pKa = 6.93YY72 pKa = 11.48ARR74 pKa = 11.84LKK76 pKa = 10.75KK77 pKa = 9.91GWKK80 pKa = 10.06LIDD83 pKa = 5.29AISKK87 pKa = 9.98PPITRR92 pKa = 11.84DD93 pKa = 3.17QALEE97 pKa = 3.87LAKK100 pKa = 9.55KK101 pKa = 7.82TNYY104 pKa = 7.79WRR106 pKa = 11.84KK107 pKa = 9.24NRR109 pKa = 11.84LLSDD113 pKa = 3.65EE114 pKa = 4.31QIEE117 pKa = 4.3IALSNGISYY126 pKa = 10.13RR127 pKa = 11.84VAYY130 pKa = 10.11GRR132 pKa = 11.84IKK134 pKa = 10.15RR135 pKa = 11.84LKK137 pKa = 9.62WPIDD141 pKa = 3.63DD142 pKa = 6.3AITTPVLTPSEE153 pKa = 4.17CAKK156 pKa = 10.27RR157 pKa = 11.84ANEE160 pKa = 3.55ASYY163 pKa = 9.69WSRR166 pKa = 11.84MRR168 pKa = 11.84ANN170 pKa = 3.31

Molecular weight: 19.74 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

56

0

56

10961

52

1551

195.7

22.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.049 ± 0.377

0.839 ± 0.173

5.894 ± 0.239

8.512 ± 0.353

4.489 ± 0.278

5.957 ± 0.379

1.624 ± 0.198

6.769 ± 0.243

9.424 ± 0.329

8.092 ± 0.333

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.029 ± 0.204

5.474 ± 0.302

2.728 ± 0.248

3.649 ± 0.216

4.744 ± 0.37

5.109 ± 0.28

6.067 ± 0.458

6.094 ± 0.288

1.761 ± 0.392

3.695 ± 0.33
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski